The effect of vitamin D supplementation and high-intensity interval training on BDNF-TrkB expression in rats fed a high-fat and high-carbohydrate diet

Zainulabden Ali Abdulrahim1, Zainulabden; Ghanbarpour Nosrati, Amir; Khalafi, Mousa

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Volume 33, Issue 2 (5-2025)

Journal of Ilam University of Medical Sciences 2025, 33(2): 86-99

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The effect of vitamin D supplementation and high-intensity interval training on BDNF-TrkB expression in rats fed a high-fat and high-carbohydrate diet

Zainulabden Zainulabden Ali Abdulrahim1¹

, Amir Ghanbarpour Nosrati ^*²

, Mousa Khalafi¹

1- Dept of Sport Sciences, Faculty of Humanities, University of Kashan, Kashan, Iran
2- Dept of Sport Sciences, Faculty of Humanities, University of Kashan, Kashan, Iran , ghanbarpour.amir@gmail.com

Abstract: (180 Views)

Introduction: Brain-derived neurotrophic factor (BDNF) has beneficial effects on non-neuronal tissues, particularly cardiovascular cells, but its effects on the BDNF-TrkB pathway in heart tissue have not been studied. Therefore, the aim of the study was to investigate the effect of high-intensity interval training (HIIT) and vitamin D supplementation (VitD) on the protein levels of cardiac BDNF-TrkB in rats fed with a high-fat and high-carbohydrate diet.
Materials & Methods: Sprague Dawley rats were divided into normal diet (ND), high fat and high carbohydrate (HFD+HC), high fat and high carbohydrate with HIIT (HFD+HC+HIIT), high fat and high carbohydrate with VitD (HFD+HC+VitD), and high fat and high carbohydrate with HIIT and VitD (HFD+HC+HII+VitD). Protein levels of BDNF and TrkB were assessed using Western blotting. Independent t-tests, one-way ANOVA, Tukey's test, and SPSS V.26 were used to analyze the data.
Results: Consumption of HFD+HC resulted in a decrease in protein levels of BDNF and TrkB in the heart compared to ND (p<0.05). On the contrary, HIIT and VitD only resulted in a non-significant increase in protein levels of BDNF and TrkB in the heart compared to the HFD+HC group. However, the combination of HIIT and VitD resulted in a significant and non-significant increase in the protein levels of TrkB (P=0.03) and BDNF (P=0.06) compared to the HFD+HC group.
Conclusion: HIIT+VD, by increasing cardiac BDNF and TrkB, may prevent the deleterious effects of high-fat and high-carbohydrate diets on this cardiac signaling, while each of these interventions alone has little effect on cardiac BDNF and TrkB.

Keywords: Brain-derived neurotrophic factor, BDNF, tropomyosin-related kinase receptor B, TrkB, Exercise, vitamin D

Full-Text [PDF 995 kb] (64 Downloads)

Type of Study: Research | Subject: Physical Education
Received: 2024/09/23 | Accepted: 2025/01/18 | Published: 2025/05/26

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Zainulabden Ali Abdulrahim1 Z, Ghanbarpour Nosrati A, Khalafi M. The effect of vitamin D supplementation and high-intensity interval training on BDNF-TrkB expression in rats fed a high-fat and high-carbohydrate diet. J. Ilam Uni. Med. Sci. 2025; 33 (2) :86-99
URL: http://sjimu.medilam.ac.ir/article-1-8411-en.html

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