[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Editorial Board
Aims& Scopes
Journal News
Articles archive::
All Issues
Current Issue
Publication Ethics::
Peer Review Process::
Indexing Databases::
For Authors::
Authors Guideline
Call for Papers
Submission Instruction
Review Process
Research Ethics Statements
Ethical issues
Author Complaint Process
For Reviewers::
Reviewers Section
Subscription::
Registration Information
Subscription Form
Contact us::
Contact Information
Contact us
Site Facilities::
Site map
Search contents
FAQ
Top 10 contents
Inform to friends
::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019
Citations63383644
h-index2719
i10-index18779
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Registered in

AWT IMAGE

AWT IMAGE
..
:: Volume 31, Issue 6 (2-2024) ::
Journal of Ilam University of Medical Sciences 2024, 31(6): 36-49 Back to browse issues page
Investigating the Cytotoxic Effect of Chitosan and Chitosan Nanoparticles in a Laboratory Environment: An In Vitro Model
Laya Ebrahimi1 , Enayat Berizi2 , Maryam Montaseri1 , Mansour Rahsepar3 , Saeid Hosseinzadeh * 4
1- Dept of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
2- Dept of Food Hygiene and Quality Control, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
3- Dept of Materials Science and Engineering, School of Engineering, Shiraz University, Shiraz, Iran
4- Dept of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran , hosseinzadeh@shirazu.ac.ir
Abstract:   (433 Views)
Introduction: Considering the broad spectrum of using nanoparticles in food coatings as a potent antimicrobial agent and their possible cytotoxic effects and accidental consumption of these toxic materials, this study was performed. The present study aimed to investigate the cytotoxicity of chitosan and nano-chitosan in vitro.
Material & Methods: This cross-sectional study was conducted in 2019 in the Laboratory of the Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. The XRD and FITR techniques were employed to study the characteristics of these nanoparticles. Moreover, two cell lines, HT-29 and Vero, were used to study the cytotoxic effects of chitosan and nano-chitosan by MTT assay, acridine orange, and ethidium bromide staining. One-way ANOVA and independent t-test were used to analyze the collected data with the help of SPSS software (version 19).
Results: Based on the obtained findings, the maximum values of XRD at the angle of θ2 were observed at 20°. The highest peak appeared at 1530 cm-1, which was associated with tensile vibration of N-P-N. The spectrum diagram of chitosan due to the tensile vibration of synthetic nanochitosan of N-H bound appeared at 1646 cm-1. The results showed a proportional increase in the cytotoxicity with time and concentration of nanoparticles in the cells.
Discussion & Conclusion: Since by increase in time and concentration of nanoparticles, toxicity was observed in cells; therefore, the time and concentration of chitosan nanoparticles are important in causing cytotoxicity. Considering the toxic effects of these nanocomposites on cancer cells, they can be used in cancer treatment, which requires further studies.
Keywords: Chitosan, Cytotoxicity, HT-29 cell, Vero cells
Full-Text [PDF 1166 kb]   (181 Downloads)    
Type of Study: Research | Subject: toxicology
Received: 2023/04/25 | Accepted: 2023/12/30 | Published: 2024/02/4
References
1. Ghytasi A, Hosseini Shekarabi SP, Rajabi Islami H, Shamsaie Mehrgan M. Dietary effects of lemon Citrus limon peel essential oil encapsulated in chitosan nanoparticles on hematological indices and antioxidant defense system of rainbow trout Oncorhynchus mykiss. Aqu Animals Nut 2021; 7: 27-41. doi: 10.22124/janb.2021.20348.1151.
2. Sabbaghi M, Maqsood Y, Khamari M, Ziaei A. Effect of mixture of chitosan coating and green tea extract on oxidative and fungal activity of walnut kernel. J Res Innovation Food Sci Ind 2013; 3: 361-74.
3. Hanifi E, Ahmadifard N, Atashbar B, Meshkini S. Effects of zinc oxide nanoparticles on photosynthetic pigments, zinc accumulation, and activity of antioxidant enzymes of Dunalilla salina. Aqua Animals Nut 2023; 8: 31-42. doi: 10.22124/janb.2023.24071.1189.
4. Gao W, Lai JC, Leung S. Functional enhancement of chitosan and nanoparticles in cell culture, tissue engineering, and pharmaceutical applications. Front Physiol 2012; 3:321. doi:10.3389/fphys.2012.00321.
5. Zarei M, Ramezani Z, Ein‐Tavasoly S, Chadorbaf M. Coating effects of orange and pegranate peel extracts combined with chitosan nanoparticles on the quality of refrigerated silver comarp fillets. J Food Proc Preserv 2015; 39: 2180-87. doi:10.1111/jfpp.12462.
6. Qi L, Xu Z, Jiang X, Li Y. Wang M. Cytotoxic activities of chitosan nanoparticles and copper-loaded nanoparticles. Bioorg Med Chem Lett 2005; 15:1397-99. doi: 10.1016/j.bmcl.2005.01.010.
7. Tyliszczak B, Drabczyk A, Kudłacik-Kramarczyk S, Bialik-Wąs K, Kijkowska R, Sobczak-Kupiec A. Preparation and cytotoxicity of chitosan-based hydrogels modified with silver nanoparticles. Colloids Surf B Biointerfaces 2017; 160: 325-30. doi: 10.1016/j.colsurfb.2017.09.044.
8. Nunes CS, Rufato KB, Souza PR, Almeida EA de, Silva MJ da, Scariot DB, et al. Chitosan/chondroitin sulfate hydrogels prepared in [Hmim][HSO4] ionic liquid. Carbohydr Polym 2017; 170: 99-106. doi.org/10.1016/j.carbpol.2017.04.073.
9. Azuma K, Osaki T, Minami S, Okamoto Y. Anticancer and anti-inflammatory properties of chitin and chitosan oligosaccharides. J Funct Biomater 2015; 6: 33-49. doi: 10.3390/jfb6010033.
10. Qi LF, Xu ZR, Li Y, Jiang X, Han XY. In vitro effects of chitosan nanoparticles on proliferation of human gastric carcinoma cell line MGC803 cells. World J Gastroenterol 2005; 11: 5136-41. doi: 10.3748/wjg. v11.i33.5136.
11. Loh JW, Yeoh G, Saunders M, Lim LY. Uptake and cytotoxicity of chitosan nanoparticles in human liver cells. Toxicol Appl Pharmacol 2010; 249:148-57. doi: 10.1016/j.taap.2010.08.029.
12. Soleimani N, Mohabati Mobarez A. Immunological effects of chitosan nanoparticles on breast cancer cells in animal model. Qom Uni Med Sci J 2017; 11: 1-9.
13. Loutfy SA, A El-Din HM, Elberry MH, Allam NG, Hasanin M, Abdellah AM. Synthesis, characterization and cytotoxic evaluation of chitosan nanoparticles: in vitro liver cancer model. Adv Nat Sci: Nanosci Nanotechnol 2016; 7:035008. doi: 10.1088/2043-6262/7/3/035008.
14. Gagnon M, Berner AZ, Chervet N, Chassard C, Lacroix C. Comparison of the Caco-2, HT-29 and the mucus-secreting HT29-MTX intestinal cell models to investigate Salmonella adhesion and invasion. J Microbiol Methods 2013; 94: 274-9. doi: 10.1016/j.mimet.2013.06.027.
15. Thirabunyanon M, Boonprasom P, Niamsup P. Probiotic potential of lactic acid bacteria isolated from fermented dairy milks on antiproliferation of colon cancer cells. Biotechnol Lett 2009; 31: 571-6. doi: 10.1007/s10529-008-9902-3.
16. Viet Quang D, Hoai Chau N. The effect of hydrothermal treatment on silver nanoparticles stabilized by chitosan and its possible application to produce mesoporous silver powder. J Powder Technol 2013; ID 281639. doi:10.1155/2013/281639.
17. Saudi A, Rafienia M, Zargar Kharazi A, Salehi H, Zarrabi A, Karevan M. Design and fabrication of poly (glycerol sebacate) ‐based fibers for neural tissue engineering: Synthesis, electrospinning, and characterization. Polym Adv Technol 2019; 30: 1427-40. doi:10.1002/pat.4575.
18. Nam KS, Kim MK, Shon YH. Chemopreventive effect of chitosan oligosaccharide against colon carcinogenesis. J Microbiol Biotechnol 2007; 17: 1546-49.
19. Lin YC, Chen GT, Wu SC. Carbohydrates-chitosan composite carrier for Vero cell culture. Cytotechnol 2016; 68: 2649-58. doi: 10.1007/s10616-016-9989-7.
20. Malathy S, Iyer P. Naringin loaded chitosan nanoparticle for bone regeneration: A preliminary in vitro study. J Nanomed Nanotechnol 2018; 9: 2. doi: 10.4172/2157-7439.1000507.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA

Ethics code: 2606026

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Ebrahimi L, Berizi E, Montaseri M, Rahsepar M, Hosseinzadeh S. Investigating the Cytotoxic Effect of Chitosan and Chitosan Nanoparticles in a Laboratory Environment: An In Vitro Model. J. Ilam Uni. Med. Sci. 2024; 31 (6) :36-49
URL: http://sjimu.medilam.ac.ir/article-1-7954-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 31, Issue 6 (2-2024) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.15 seconds with 41 queries by YEKTAWEB 4646