Comparison of Antimicrobial Properties and Toxicity of Natural S3 Peptide with Horseshoe Crab Amoebocyte Origin and its Mutants

Rezaei, Sadegh; Hadadian, Shahin; Khavari nejad, Ramazan ali; Norouzian, Dariush

doi:10.52547/sjimu.29.4.60

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Volume 29, Issue 4 (10-2021)

Journal of Ilam University of Medical Sciences 2021, 29(4): 60-73

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Comparison of Antimicrobial Properties and Toxicity of Natural S3 Peptide with Horseshoe Crab Amoebocyte Origin and its Mutants

Sadegh Rezaei¹

, Shahin Hadadian ^*

², Ramazan ali Khavari nejad¹

, Dariush Norouzian³

1- Dept of Biology, Islamic Azad University, Science and Research Branch, Tehran, Iran
2- Dept of Nanotechnology, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran , hadadian@yahoo.com
3- Dept of Nanotechnology, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran

Abstract: (1646 Views)

Introduction: Antimicrobial peptides (AMPs) are compounds with antimicrobial properties that are studied widely due to the development of resistance of pathogenic bacteria to antibiotics. In the present study, the toxicity and antimicrobial effects of two natural monomeric peptides (S3 and S∆3) were compared with S3-S∆3 hybrids and S3 tetramers.
Material & Methods: Protein hybrids (S∆3S3-2mer-GS) S3-S∆3 and tetramer protein S3 (S3-4mer-GS) were expressed in E. coli. BL21 (DE3). Following that, the presence of mutant peptides was confirmed, and their antimicrobial activity was compared and evaluated with S3 and S∆3 monomers. Finally, the toxicity of tetramer and hybrid made on the MDA-MB-231 cell line was evaluated and compared.
Findings: The toxicity of the hybrid was slightly increased, compared to the tetramer for eukaryotic cells; however, this increase was negligible at the active concentration of this protein. Cell survival for hybrids was lower for S3 and SΔ3; nonetheless, cell survival for each sequence decreased with increasing time. Furthermore, the inhibition of hybrid microbial growth was improved and compared with tetramer and S3-SΔ3. It was found that an increase in the positive charge of the hybrid protein did not have a toxic effect on the host bacteria.
Discussion & Conclusion: Due to the appropriate expression and increased antimicrobial activity and negligible cytotoxicity, the hybrid peptide S3-S∆3 and tetramer S3 can be considered an effective production strategy to obtain AMPs.

Keywords: Antimicrobial peptides, Factor c, Horseshoe crab, S3-S∆3 protein hybrid, Tetramer S3 peptide

Full-Text [PDF 771 kb] (706 Downloads)

Type of Study: Research | Subject: Medical microbiology
Received: 2021/05/4 | Accepted: 2021/09/1 | Published: 2021/11/1

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Rezaei S, Hadadian S, Khavari nejad R A, Norouzian D. Comparison of Antimicrobial Properties and Toxicity of Natural S3 Peptide with Horseshoe Crab Amoebocyte Origin and its Mutants. J. Ilam Uni. Med. Sci. 2021; 29 (4) :60-73
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