Effect of High-Intensity Interval Training following Ischemia-Reperfusion Injury in Heart on Hand 2 and
 Phosphatidylinositol-3-Kinase
 Genes in Male Rats

Heidarianpour, Ali; Mehrialvar, Yaghoub; Mehrialvar, Shiva

doi:10.29252/sjimu.28.4.79

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Volume 28, Issue 4 (10-2020)

Journal of Ilam University of Medical Sciences 2020, 28(4): 79-89

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Effect of High-Intensity Interval Training following Ischemia-Reperfusion Injury in Heart on Hand 2 and Phosphatidylinositol-3-Kinase Genes in Male Rats

Ali Heidarianpour ^*

¹, Yaghoub Mehrialvar²

, Shiva Mehrialvar²

1- Dept of Exercise Physiology, Faculty of Physical Education and Sport Science, Bu-Ali Sina University, Hamadan, Iran , Heidarian317@gmail.com
2- Dept of Exercise Physiology, Faculty of Physical Education and Sport Science, Bu-Ali Sina University, Hamadan, Iran

Abstract: (2842 Views)

Introduction: Experimental and clinical studies have shown that exercise training is one of the most effective strategies for reducing the progression of cardiomyopathy and decreasing the incidence of cardiovascular complications and mortality due to myocardial ischemia. This study aimed to investigate the role of high-intensity interval training (HIIT) in the expression of Hand2 and Phosphatidylinositol-3-kinase (PI3K) genes in cardiac ischemia rats.

Materials & Methods: In this study, 28 male Wistar rats (200-250 g) were randomly divided into four groups of sham, ischemia, exercise, and exercise-ischemia. Myocardial infarction was performed by the closure of the left anterior descending artery for 30 min. In total, 40-min HIIT (each interval consisted of 4-min very high intensity running with approximately 85% to 90% maximum rate of oxygen consumption [VO2_max] followed by 2-min active recovery with approximately 50% to 60% VO2_max) were performed three days a week for eight weeks.
Ethics code: IR.SSRI.REC.1396.134

Findings: The results showed that the expression level of the Hand2 gene in the ischemia-training group was significantly increased, compared to the sham (P=0.001) and ischemia (P=0.001) groups. Furthermore, the concentration of PI3K in the exercise and exercise-ischemia groups was significantly increased, compared to the sham (P=0.001) and ischemia (P=0.001) groups. However, there was a decrease in the ischemia group, compared to the other groups (P<0.05).

Discussions & Conclusions: The findings of the present study showed that HIIT leads to increased expression of the Hand2 gene and PI3K. Furthermore, the HIIT results in physiological hypertrophy. On the other hand, the pathological hypertrophy of these variables is reduced in this study.

Keywords: Exercise training, Myocardial ischemia, Pathological hypertrophy, Physiological hypertrophy, Phosphatidylinositol-3-kinase

Full-Text [PDF 994 kb] (821 Downloads)

Type of Study: Research | Subject: Physical Education
Received: 2019/11/3 | Accepted: 2020/04/19 | Published: 2020/10/31

References

1. Tao L, Bei Y, Zhang H, Xiao J, Li X. Exercise for the heart signaling pathways. Oncotarget 2015; 6:20773. doi.10.18632/oncotarget.4770

2. Pluim B, Zwinderman AH, Laarse A, Wall EE. The athletes heart a meta-analysis of cardiac structure and function. Circulation 2000; 101:336-44. doi. 10.1161/ 01.cir.101.3.336

3. Palabiyik O, Tastekin E, Doganlar ZB, Tayfur P, Dogan A, Vardar SA. Alteration in cardiac PI3K/Akt/mTOR and ERK signaling pathways with the use of growth hormone and swimming and the roles of miR21 and miR133. Biomed Rep2019; 10:97-106. doi. 10.3892/br.2018.1179

4. Sanada S, Komuro I, Kitakaze M. Pathophysiology of myocardial reperfusion injury preconditioning, postconditioning and translational aspects of protective measures. Am J Heart Circul Physio l2011;301: 723-41. doi. 10.1152/ ajpheart. 00553.2011

5. Lloyd D, Adams RJ, Brown TM, Carn-ethon M, Dai S, Simone G, et al. Executive summary heart disease and stroke statistics 2010 update a report from the American heart association. Circulation 2010; 121: 948-54. doi.10.1161/CIR. 0000000000000 757

6. Whitcomb J, Gharibeh L, Nemer M. From embryogenesis to adulthood critical role for GATA factors in heart development and function. IUBMB Life2019; 2:81-6. doi.10.1002/iub.2163

7. George R, Firulli AB. Hand factors in cardiac development. Anatom Rec2019; 302:101-7. doi: 10.1002/ar.23910

8. Beisvag V, Kemi OJ, Arbo I, Loennechen JP, Wisloff U, Langaas M. Pathological and physiological hypertrophies are regulated by distinct gene programs. European J Car-diovasPreve Rehabil2009; 16:690-7. doi. 10.1097/HJR.0b013e32833158a2

9. Lu C, Gong HR, Liu XY, Wang J, Zhao CM, Huang RT. A novel HAND2 loss-of-function mutation responsible for tetralogy of Fallot. Int J Mole Med2016; 37:445-51. doi.10.3892/ijmm.2015.2436

10. Gharaat M, Kashef M, Jameie B, Rajabi H. [Effect of endurance a nd high intensity interval swimming training on cardiac structure and Hand2 expression of Rats]. SSUJ 2017; 25:748-58.

11. Lu K, Wang L, Wang C, Yang Y, Hu D, Ding R. Effects of high intensity interval versus continuous moderate intensity aerobic exercise on apoptosis, oxidative stress and metabolism of the infarcted myocardium in a Rat model. Mole Med Rep 2015; 12:2374-82. doi.10.4103/ jrms.JRMS_292_18

12. Fathi M, Gharakhanlou R. [Effect of Endurance training on Hand2 gene expression in left ventricle of male Rats]. Sport Physiol 2015; 7: 57-68. (Persian)

13. Shioi T, Kang PM, Douglas PS, Hampe J, Yballe CM, Lawitts J. The conserved phosphoinositide 3‐kinase pathway determ-ines heart size in Mice. EMBO J2000; 19:2537-48. doi.10.1093/emboj/ 19.11.2537

14. Huenges K, Pokorny S, Berndt R, Cremer J, Lutter G. Transesophageal echocardiography in swine establishment of a baseline. Ult Med Biol2017; 43:974-80. doi.10.1016/j.ultrasmedbio.2016.12.011

15. Hoydal M, Wisloff U, Kemi OJ, Ellin-gsen O. Running speed and maximal oxy-gen uptake in rats and Mice practical imp-lications for exercise training. European J Cardiovas Preve Rehabil2007; 4:753-60. doi.10.1097/HJR.0b013e3281eacef1

16. Wisloff U, Loennechen JP, Currie S, Smith GL, Ellingsen O. Aerobic exercise reduces cardiomyocyte hypertrophy and increases contractility Ca2+ sensitivity and SERCA-2 in rat after myocardial infarction. Cardiovas Res2002;54: 162-74.doi.10. 1016/s0008-6363(01)00565-x

17. Sun Y, Wang J, Qiu XB, Yuan F, Li RG, Xu YJ, et al. A HAND2 loss of function mutation causes familial ventricular septal defect and pulmonary stenosis. Gen Genom Genet 2016; 6:987-92. doi.10.1534/g3. 115.026518

18. O'Neill B, Kim J, Wende AR, Theobald HA, Tuinei J, Buchanan J, Guo A, Zaha VG, Davis DK, Schell JC, Boudina S. A conserved role for phosphatidylinositol 3-kinase but not Akt signaling in mitochon-drial adaptations that accompany physiol-ogical cardiac hypertrophy. Cell Metab 2007; 6:294-306. doi. 10.1016/ j.cmet. 2007.09.001

19. Owen K, Pretorius L, McMullen JR. The protective effects of exercise and phosphor-inositide 3-kinase p110α in the failing heart. Clin Sci2009; 116:365-75. doi.10. 1042/CS20080183

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Heidarianpour A, Mehrialvar Y, Mehrialvar S. Effect of High-Intensity Interval Training following Ischemia-Reperfusion Injury in Heart on Hand 2 and Phosphatidylinositol-3-Kinase Genes in Male Rats. J. Ilam Uni. Med. Sci. 2020; 28 (4) :79-89
URL: http://sjimu.medilam.ac.ir/article-1-6140-en.html

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