[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 26, Issue 1 (5-2018) ::
sjimu 2018, 26(1): 73-84 Back to browse issues page
Effects of Ginger (Zingiber Officinale) Aqueous Extract on the Levels of Hepatic Enzymes, Biochemical Parameters, and Histological Changes in Male Wistar Strain Rats Following Treatment with Streptozotocin
Tahmoores Shahrivar 1 , Mokhtar Mokhtari 2, Vally Alipour 3
1- Dept of Biology , Faculty of Sciences , Islamic azad University , Kazerun Branch , Kazerun , Iran
2- Dept of Biology , Faculty of Sciences , Islamic azad University , Kazerun Branch , Kazerun , Iran , M.mokhtari246@yahoo.com
3- Dept of Environmental Health, Faculty of Environmental Health, Hormozgan University of Medical Sciences, Hormozgan, Iran
Abstract:   (598 Views)
Introduction: Diabetes induces leakage of hepatic enzymes from cytosol into the circulation and increases the plasma levels of these enzymes. In this study, the impacts of ginger (Zingiber Officinale) aqueous extract on the levels of liver enzymes, biochemical parameters, and histological changes in male rats were assessed following induction of diabetes by Streptozotocin.
 
Materials & Methods: 42 male Wistar rats were divided into 6 groups: the control (left untreated), diabetic control (received 70 mg/kg Streptozotocin once at the beginning of the experiment), the experimental groups 1 and 2 (daily received 250 and 500 mg/kg ginger aqueous extract, respectively), the experimental groups 3 and 4 (first received streptozotocin once, and then a daily dose of 250 and 500 mg/kg extract). Oral administration of aqueous extract continued over a period of two months. 48 hours after the last administration, blood samples were prepared and used for measurement of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and serum levels of albumin and total protein. Also, hepatic histological study was carried out in hematoxylin-eosin staining method.
 
Findings: The serum levels of AST, ALT, and ALK enzymes in diabetic control group (recipient of 70mg/kg Streptozotocin) and the AST enzyme concentration in experimental group 2 (recipient of 500 mg/kg Ginger extract alone) increased significantly compared to the control group
 (p<0.05). In contrast, the levels of ALT and ALP enzymes declined significantly in experimental group 1 (recipient of 250mg/kg extract alone) relative to the control p (< 0.05). Serum concentrations of total protein and albumin reduced significantly in the experimental group 2 in comparison with the control group. Also, In experimental groups 3 and 4 (recipient of 250 and 500 mg/kg ginger extract and streptozotocin) relative to the diabetic control group, the levels of AST , ALT and ALP indicated a significant reduction, while the concentration of total proteins increased significantly in experimental group 3          (p < 0.05). Finally, histological study revealed cellular necrosis, cellular inflammation, and vacuolar fat accumulation, disintegration of portal spaces and formation of large intercellular spaces in diabetic control relative to the control group. Such disorders were less significant in groups receiving streptozotocin along with 500mg/kg doses of extract compared to the group receiving streptozotocin alone.
 
 Discussion & Conclusions: Due to the presence of compounds such as gingerol, shoagol, and anthocyanins, ginger aqueous extract can possibly reduce hepatic toxicity caused by streptozotocin and improve the serum levels of liver enzymes.
Keywords: ginger, streptozotocin, AST, ALT, ALP, biochemical parameters, rat
Full-Text [PDF 923 kb]   (284 Downloads)    
Type of Study: Research | Subject: animal physiology
Received: 2017/09/1 | Accepted: 2018/01/9 | Published: 2018/05/13
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shahrivar T, Mokhtari M, Alipour V. Effects of Ginger (Zingiber Officinale) Aqueous Extract on the Levels of Hepatic Enzymes, Biochemical Parameters, and Histological Changes in Male Wistar Strain Rats Following Treatment with Streptozotocin . sjimu. 2018; 26 (1) :73-84
URL: http://sjimu.medilam.ac.ir/article-1-4542-en.html


Volume 26, Issue 1 (5-2018) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
Persian site map - English site map - Created in 0.24 seconds with 31 queries by YEKTAWEB 3767