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:: Volume 26, Issue 6 (3-2019) ::
sjimu 2019, 26(6): 156-163 Back to browse issues page
Investigating the distribution of TCF7L2 (rs7903146) gene polymorphism in type 2 diabetic patients in West of Iran Mazandaran
Mohammad Shekarzadeh1 , Abas Mohammad poor1, Mehdi Abasi ROSHAN2, Mehran Rajabi sahneh saraie2, Mostafa Daryaie 3
1- Dept of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
2- Dept of Genetics, Faculty of Biology, Sana Institute of Higher Education, Sari, Iran
3- Dept of Genetics, Faculty of Biology, Sana Institute of Higher Education, Sari, Iran , mostafa.daryaei71@gmail.com
Abstract:   (499 Views)

Introduction: Type 2 diabetes mellitus is a complex heterogeneous group of metabolic conditions characterized by increased levels of blood glucose due to impairment in insulin action and/or insulin secretion. The TCF7L2 gene is considered as one of the major genes susceptible to diabetes.The single nucleotide polymorphism rs7903146 is located in intron 3 of this gene, which is significantly effective in type 2 diabetes.
Materials and methods: In this case-control study, 141 patients and 86 healthy subjects were present. 5cc peripheral blood of diabetics was collected in EDTA-containing tubes. Genomic DNA was extracted using salting-out method and polymorphism genotype was investigated using PCR-RFLP method.
Findings: Genotype and allele frequencies of the rs7903146 polymorphism differed significantly between type 2 diabetic patients and non-diabetic subjects (P = 0.0468 and P = 0.0474 , respectively). The frequency of T allele was 54.5% in type 2 diabetes group and 39.5% in non-diabetic subjects, and this allele was significantly associated with type 2 diabetes risk (OR = 1.8333, 95% CI 1.0456 – 3.2144).
Discussion & Conclusion: Our results confirmed the association between the rs7903146 polymorphism of the TCF7L2 gene and the increased risk of type 2 diabetes in the west-Mazandaran, Iran.
 

Keywords: Type 2 diabetes mellitus, rs7903146 polymorphism, TCF7L2 gene, PCR-RFLP
Full-Text [PDF 630 kb]   (179 Downloads)    
Type of Study: Research |
Received: 2017/07/9 | Accepted: 2017/08/16
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Shekarzadeh M, Mohammad poor A, Abasi ROSHAN M, Rajabi sahneh saraie M, Daryaie M. Investigating the distribution of TCF7L2 (rs7903146) gene polymorphism in type 2 diabetic patients in West of Iran Mazandaran. sjimu. 2019; 26 (6) :156-163
URL: http://sjimu.medilam.ac.ir/article-1-4427-en.html


Volume 26, Issue 6 (3-2019) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
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