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:: Volume 26, Issue 4 (11-2018) ::
sjimu 2018, 26(4): 104-115 Back to browse issues page
Effects of Dexamethasone on Regeneration Process after Sciatic Nerve Transect and Acellular Scaffold Graft in a Rat Animal Model
Mohammad bagher Ghayour1 , Arash Abdolmaleki2, Morteza Behnamrasouli 3, Ali Moghimi1, Naser Mahdavishahri1
1- Dept of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
2- . Dept of Engineering Sciences, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran
3- Dept of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran , davidhay217@gmail.com
Abstract:   (902 Views)

Introduction: Peripheral nervous system has an innate regenerative ability, and in severe injuries leading to nerve destruction, repair does not occur spontaneously and medical intervention is required. In this regard, dexamethasone is one of useful drugs prescribed for neurological recovery after spinal cord injury. The objective of the present study was to assess the effect of dexamethasone on functional recovery and histological changes following sciatic nerve transect and regeneration with the acellular scaffolds in male Wistar rats.
 
Materials & Methods: Fifty male Wistar rats were randomly divided into five groups of 10. The groups included: negative control group(left sciatic nerve was transecte), sham-operated group) surgical procedure without the nerve transect ), and experimental groups 1 and 2 (sciatic nerve was transected and regenerated with acellular nerve scaffold) treated with 1 and 2 mg/kg of dexamethasone, respectively. Experimental group 3 (sciatic nerve was transected and regenerated with acellular nerve scaffold) was injected with vehicle. Then, 1 cm of left sciatic nerve was exposed and replaced with a piece of scaffold. Behavioral, electrophysiological, and morphological alterations were evaluated during the experiment.
 
Findings: with dexamethasone in comparison with the control group (P < 0.05). Analysis of gastrocnemius muscle mass in groups treated with dexamethasone in comparison with negative control group showed that muscle atrophy was reduced; also the dexamethasone-treated group presented significantly greater nerve fiber, axon diameter, and myelin sheath thickness (P < 0.05).
 
Discussion & Conclusions: The results showed that the administration of dexamethasone after sciatic nerve transects and grafting the acellular scaffold increased motor function in a rat animal model.

Keywords: Dexamethasone, Sciatic nerve, Regeneration, Acellular scaffold, Peripheral nerve injury
Full-Text [PDF 1098 kb]   (517 Downloads)    
Type of Study: Research | Subject: animal physiology
Received: 2017/04/18 | Accepted: 2017/06/12
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Ghayour M B, Abdolmaleki A, Behnamrasouli M, Moghimi A, Mahdavishahri N. Effects of Dexamethasone on Regeneration Process after Sciatic Nerve Transect and Acellular Scaffold Graft in a Rat Animal Model . sjimu. 2018; 26 (4) :104-115
URL: http://sjimu.medilam.ac.ir/article-1-4263-en.html


Volume 26, Issue 4 (11-2018) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
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