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Breast Cancer And Glutathione s-transferaseGenetic Polymorphism
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Mitra Azizian *   , Bahram Yaghmaei |
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Abstract: (15043 Views) |
| Introduction: Breast cancer is one of the most common known cancers among women of all the racial groups. Thus, a study on both the genetic and environmental factors provides a new chance for us to comprehend the risks of breast cancer. Molecular studies were performed in order to elucidate the relationship between three polymorphic metabolic enzymes with breast cancer.
Materials & Methods: In this case-control study, the polymorphisms of glutathione s-transferase (GST) M1, T1 and P1 were analyzed by PCR in genomic DNA from blood samples of 31 female breast cancer patients against 46 women as the control group. To investigate any possible relationships between genotype in GSTT1 and GSTM 1 a Mult. PCR method was used, while for genotype in GSTP 1 a PCR-RFLP method was designed and followed. To determine the relationship between genotypes of GSTT1, GSTM1, and GSTP1 with breast-canser risks, the OR (odd ratio) was calculated with a 95 certainty distance.
Findings: GSTT1 null genotype was a risk factor (odd ratio (OR), 4.75, 95% confidence interval (CI), 1.66-13.51). Hetrozygoute polymorphism of GSTP1 at nucleotide 313 was associated (OR, 3.85 95% CI, 1.39- 10.61). GSTM1 null genotype was not associated with the breast cancer risk.
Discussion & Conclusion: According to the achievements of the study, we conclude that GSTP1 and GSTT1 genes could play a role in carcinogenesis in the breast cancer. |
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| Keywords: breast cancer, glutathione s-transferase, polymorphism |
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Full-Text [PDF 329 kb]
(3971 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2009/08/5 | Accepted: 2015/04/20 | Published: 2015/04/20
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