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Potential Capacity of Cord Blood-Derived Mesenchymal Stem Cells in the Regeneration of Bone Defects in Rabbits
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S Heidari keshel   , M Rezaei Tavirani * 1, M Ebrahimi , Gh r Behrozi , A a Ronaghi , J Dost mohammad , R Roozafzoon , A Moaieri , Sh Mohammadpor |
| 1- , rezaei.tavirani@ibb.ut.ac.ir |
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Abstract: (15063 Views) |
| Introduction: Periodontal disease in its advanced chronic forms, can involve all or parts of periodontium. While periodontal diseases are accompanied with extensive destruction of periodontal tissues (include-ing cementum, alveolar bone, gingival and periodontal ligament), the tissue has a limited ability to regenerate itself. Due to the limited regenerating capacity of the tissue, application of cells with high pote-ncy of regeneration may be for the treatm-ent of periodontal disease.
Materials & Methods: Cord blood was collected from the umbilical cord vein of 30 mothers who gave informed consent. The isolated mononuclear cell layer was washed in PBS, and then re-suspended in growth medium. The cells were analyzed with flowcytometry for superficial mar-kers. Critical-sized (8 mm) calvarial defe-cts were created in the parietal bone of ad-ult rabbit. Defects were either left empty, treated with a collagen alone, or a collagen with human cord blood-derived mesenc-hymal stem cells (USSCs). Histology and histomorphometry were performed.
Finding: USSCs were positive for the markers CD73 and CD105, negative for the marker CD34 and had the normal kary-otype 44XX. Quality of the regenerated bone and its connection to surrounding bone tissue were significantly improved in the treated rabbits.
Discussion & Conclusions: Results of the study demonstrated that from the viewpoint of histomorphometric, the rate of lesion regeneration was about 90% higher in the treatment group and the complete reconst-ruction was accomplished after 8 weeks |
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| Keywords: bone, USSCs, stem cell |
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Full-Text [PDF 680 kb]
(2311 Downloads)
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Type of Study: Research |
Subject:
pathology
Received: 2013/06/2 | Accepted: 2013/09/1 | Published: 2013/09/1
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