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Study of Antibiotical Effects of Scrophularia Striata With Tetracyclin Aqueous Extract on E. Coli in Comparison With Tetracyclin
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M Zamaninan Azodi   , Ar Ardeshiry Lajimi , M, Rezaei Tavirani , M, Rezaei Tavirani * 1, R Khodarahmi |
| 1- , tavirani_m@yahoo.com |
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Abstract: (20846 Views) |
| Introduction: Since bacteria became resistant to some types of antibiotics, it is prominent to find more reliable sources against them. In view of the fact that plants have been shown verity of biological eff-ects, they could be promising substitutions. Inasmuch as Scrophularia has shown differ-rent biological properties such as antimic-robial, antitumor and anti-inflammatory activities, its effect on Escherichia coli has been evaluated.
Material & Methods: Antibiotic effects of both filtered and nonfiltered extract of Scro-phularia striata on Escherichia coli as gram-negative bacterium were evaluated by spectrophotometery method. Simultaneou-sly tetracycline was used as a positive control at different concentrations. In addi-tion, MTT assay was applied for cell surv-ival determination.
Findings: Our findings indicate that conc-entrations between 1 to 20 µg/ml of the extract have significant antibiotic activities there is no noticeable difference between filtered and nonfiltered antibiotic activities of this extract. Moreover, this effect is totally different from inducing properties of the extract.
Discussion & Conclusion: In conclusion, 5 µg/ml of the extract has a significant antibiotic activity whereas there is not such an effect on fibroblast cells. In view of the fact that this concentration of the extract has a potential antibiotic activity accomp-anied with considerable low side effects on human cells, Scrophularia striata extract can be regard as a potential antibiotic agent in the future. |
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| Keywords: scrophularia striata; antim-icrobial effects; escherichia coli; human fibroblast cell line |
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Full-Text [PDF 361 kb]
(3529 Downloads)
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Type of Study: case report |
Subject:
Microbiology
Received: 2013/02/24 | Accepted: 2013/03/17 | Published: 2013/03/17
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