[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Editorial Board
Aims& Scopes
Journal News
Articles archive::
All Issues
Current Issue
Publication Ethics::
Peer Review Process::
Indexing Databases::
For Authors::
Authors Guideline
Call for Papers
Submission Instruction
Review Process
Research Ethics Statements
Ethical issues
Author Complaint Process
For Reviewers::
Reviewers Section
Subscription::
Registration Information
Subscription Form
Contact us::
Contact Information
Contact us
Site Facilities::
Site map
Search contents
FAQ
Top 10 contents
Inform to friends
::
Google Scholar Metrics

Citation Indices from GS

AllSince 2020
Citations69133468
h-index2819
i10-index20981
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Registered in

AWT IMAGE

AWT IMAGE
..
:: Volume 31, Issue 4 (10-2023) ::
Journal of Ilam University of Medical Sciences 2023, 31(4): 97-105 Back to browse issues page
A comparative study on the scolicidal effect of amino-bromophenylcoumarin and amino-hydroxyphenylcoumarin on hydatid cyst protoscolexes in vitro
Yousef Panahi * 1, Roghayeh Norouzi2 , Reza Teimuri Mofrad3 , Shabnam Soleimani4 , Vahid Mahmoodi4 , Shabnam Tahmasebi3
1- Dept of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran , y.panahi@tabrizu.ac.ir
2- Dept of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
3- Dept of Organic Chemistry and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran
4- Dept of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Abstract:   (720 Views)
Introduction: Echinococcus granulosus is the causative agent of hydatid cysts, which are distributed worldwide. Nowadays, much research is being done to inactivate hydatid cyst protoscoleces. This study was conducted with the aim to compare the scolicidal effect of two chemical compounds 12 and 2 on hydatid cyst protoscoleces in vitro.
Material & Methods: The scolicidal activity of two amini-hydrox-phenylcoumarin and amini-bromo-phenylcoumarin at concentrations of 1.2, 1.5, 1.10 and 1.100 after 15, 30 and 60 minutes of incubation was tested separately and each of the experiments was repeated three times. The viability of protoscoleces was checked with 0.1% eosin dye under a light microscope. Subsequently data were analyzed using GraphPad version 5 software and two-way repeated measures ANOVA and values  (P > 0.05) was considered a significant level.
Findings: The results of this study showed that the chemical compound amini-bromophenylcoumarin at a concentration of 1.10 destroyed 100% of protoscoleces after 60 minutes of exposure compared to the control group, but amini-hydroxophenyl-coumarin, at a concentration of 1.100 could destroy 98% of protoscolecia after 60 minutes of exposure.
Discussion & Conclusion: The results of the present study showed that amini-bromophenylcoumarin had stronger protoscolicidal effects than amini-hydroxophenyl-coumarin. However, further studies and in vivo studies are needed to evaluate the efficacy of these chemical compounds.

 
Keywords: Coumarin Derivatives, Hydatid cyst, In vitro, Protoscolicidal
Full-Text [PDF 507 kb]   (450 Downloads)    
Type of Study: Research | Subject: environmental healt
Received: 2023/04/19 | Accepted: 2023/10/22 | Published: 2023/10/7
References
1. Norouzi R, Ataei A, Hejazy M, Noreddin A, El Zowalaty ME. Scolicidal effects of nanoparticles against hydatid cyst protoscolices in vitro. Int J Nanomedicine 2020; 15:1095-100. doi: 10.2147/IJN.S228538. eCollection 2020.
2. Ahmadpour E, Godrati-Azar Z, Spotin A, Norouzi R, Hamishehkar H, Nami S, et al. Nanostructured lipid carriers of ivermectin as a novel drug delivery system in hydatidosis. Parasit Vectors 2019; 12:469. doi: 10.1186/s13071-019-3719-x.
3. Naseri M, Akbarzadeh A, Spotin A, Akbari NAR, Mahami-Oskouei M, Ahmadpour E. Scolicidal and apoptotic activities of albendazole sulfoxide and albendazole sulfoxide-loaded PLGA-PEG as a novel nanopolymeric particle against Echinococcus granulosus protoscoleces. Parasitol Res 2016; 115:4595-603. doi: 10.1007/s00436-016-5250-8.
4. Walker M, Rossignol JF, Torgerson P, Hemphill A. In vitro effects of nitazoxanide on Echinococcus granulosus protoscoleces and metacestodes. J Antimicrob Chemother 2004; 54:609-16. doi: 10.1093/jac/dkh386.
5. Maggiore MA, Albanese AA, Gende LB, Eguaras MJ, Denegri GM, Elissondo MC. Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes. Parasitol Res 2012; 110:1103-12. doi: 10.1007/s00436-011-2595-x.
6. Anthony J-P, Fyfe L, Smith H. Plant active components–a resource for antiparasitic agents? Trends Parasitol 2005; 21:462-8. doi: 10.1016/j.pt.2005.08.004.
7. Fylaktakidou KC, Hadjipavlou-Litina DJ, Litinas KE, Nicolaides DN. Natural and synthetic coumarin derivatives with anti-inflammatory/antioxidant activities. Curr Pharm Des 2004; 10:3813-33. doi: 10.2174/1381612043382710.
8. Bonsignore L, Loy G, Secci D, Calignano A. Synthesis and pharmacological activity of 2-oxo-(2H) 1-benzopyran-3-carboxamide derivatives. Eur J Med Chem 1993; 28:517-20. doi:10.1016/0223-5234(93)90020-F.
9. Cingolani G, Gualtieri F, Pigini M. Researches in the field of antiviral compounds. Mannich bases of 3-hydroxycoumarin. J Med Chem 1969; 12:531-2. doi:10.1021/jm00303a616.
10. Wu JY, Fong WF, Zhang JX, Leung CH, Kwong HL, Yang MS, et al. Reversal of multidrug resistance in cancer cells by pyranocoumarins isolated from Radix Peucedani. Eur J Pharmacol 2003; 473:9-17. doi: 10.1016/s0014-2999(03)01946-0.
11. Perrella FW, Chen SF, Behrens DL, Kaltenbach RF 3rd, Seitz SP. Phospholipase C inhibitors: a new class of cytotoxic agents. J Med Chem 1994; 37:2232-7. doi: 10.1021/jm00040a016.
12. Emmadi NR, Atmakur K, Chityal GK, Pombala S, Nanubolu JB. Synthesis and cytotoxicity evaluation of highly functionalized pyranochromenes and pyranopyrans. Bioorg Med Chem Lett 2012; 22:7261-4. doi: 10.1016/j.bmcl.2012.09.018.
13. Mungra DC, Patel MP, Rajani DP, Patel RG. Synthesis and identification of β-aryloxyquinolines and their pyrano [3, 2-c] chromene derivatives as a new class of antimicrobial and antituberculosis agents. Eur J Med Chem 2011; 46:4192-200. doi: 10.1016/j.ejmech.2011.06.022.
14. Teimuri-Mofrad R, Esmati S, Tahmasebi S, Gholamhosseini-Nazari M. Bisferrocene-containing ionic liquid supported on silica coated Fe3O4: A novel nanomagnetic catalyst for the synthesis of dihydropyrano [2, 3-c] coumarin derivatives. J Organomet Chem 2018; 870:38-50. doi: 10.1016/j.jorganchem.2018.06.007.
15. Rouhani S, Parvizi P, Spotin A. Using specific synthetic peptide (p176) derived AgB 8/1-kDa accompanied by modified patient’s sera: a novel hypothesis to follow-up of Cystic echinococcosis after surgery. Med Hypotheses 2013; 81:557-60. doi: 10.1016/j.mehy.2013.07.003.
16. Fakhar M, Chabra A, Rahimi-Esboei B, Rezaei F. In vitro protoscolicidal effects of fungal chitosan isolated from Penicillium waksmanii and Penicillium citrinum. J Parasit Dis 2015; 39:162-7. doi: 10.1007/s12639-013-0300-y.
17. Kohansal MH, Nourian A, Rahimi MT, Daryani A, Spotin A, Ahmadpour E. Natural products applied against hydatid cyst protoscolices: A review of past to present. Acta Trop 2017; 176:385-94. doi: 10.1016/j.actatropica.2017.09.013.
18. Williams RJ. Determination of the Value of Formalin and Boiling Water as Taeniid Ovicides. Res Vet Sci 1963; 4:550-5. doi:10.1016/S0034-5288(18)34841-0.
19. Besim H, Karayalcin K, Hamamci O, Güngör C, Korkmaz A. Scolicidal agents in hydatid cyst surgery. HPB Surg 1998; 10:347-51. doi: 10.1155/1998/78170.
20. Caglar R, Yuzbasioglu MF, Bulbuloglu E, Gul M, Ezberci F, Kale IT. In vitro effectiveness of different chemical agents on scolices of hydatid cyst. J Investig Surg 2008; 21:71-5. doi: 10.1080/08941930701883640.
21. Borazan E, Gökalp MA, Zer Y, Aksoy N, Aytekin A, Yılmaz L. Time-related comparison of scolicidal activity of the different substances used in hydatid cyst. East J Med 2019; 24:457-62. doi: 10.5505/ejm.2019.04706.
22. Puryan K, Karadayi K, Topcu O, Canbay E, Sumer Z, Turan M, et al. Chlorhexidine gluconate: an ideal scolicidal agent in the treatment of intraperitoneal hydatidosis? World J Surg 2005; 29:227-30. doi: 10.1007/s00268-004-7587-x.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Panahi Y, Norouzi R, Teimuri Mofrad R, Soleimani S, Mahmoodi V, Tahmasebi S. A comparative study on the scolicidal effect of amino-bromophenylcoumarin and amino-hydroxyphenylcoumarin on hydatid cyst protoscolexes in vitro. J. Ilam Uni. Med. Sci. 2023; 31 (4) :97-105
URL: http://sjimu.medilam.ac.ir/article-1-7946-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 31, Issue 4 (10-2023) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.16 seconds with 41 queries by YEKTAWEB 4671