Protective Effects of Simvastatin against Doxorubicin-Induced Changes in Mice
Testicular Tissue
|
Golamreza Najafi * 1, Ali Shalizar Jalali2 , Mahla Mohammadi3 |
1- urmia universityDept of Basic Sciences, Faculty of Veterinary Medicine, UrmiaUniveraity, Urmia, Iran , g.najafi2006@yahoo.com 2- urmia universityDept of Basic Sciences, Faculty of Veterinary Medicine, UrmiaUniveraity, Urmia, Iran 3- Dept of Basic Sciences, Faculty of Veterinary Medicine, UrmiaUniveraity, Urmia, Iran |
|
Abstract: (5798 Views) |
Introduction: Doxorubicin (DOX), a widely used antineoplastic compound, is associated with testicular damage and infertility. This study was carried out to disclose the possible preventive effects of simvastatin (SIM), a lipid lowering agent with antioxidant and anti-inflammatory activities, against testicular morphometric-stereological changes due to DOX treatment in mice.
Materials & methods: Male mice, five weeks old, were randomly divided into four groups of six mice each. The DOX was administered to two groups of mice in 5 equal intraperitoneal injections over a period of 4 weeks (4 mg/kg). One of these groups received 5 equal oral doses of SIM (12 mg/kg) along with DOX. A vehicle-treated control group and a SIM control group were also included. Morphometric measurement was determined using calibrated ocular micrometer.
Findings: Histomorphometrical examinations revealed significant reductions in the seminiferous tubules diameters and their epithelial heights along with an increase in testicular capsule thickness and interstitial space widening in DOX-treated mice. All above-mentioned parameters recovered significantly by SIM co-administration.
Discussion & conclusions: These results indicated that SIM may have a preventive role against morphometric-stereological changes caused by DOX in mouse testis.
|
|
Keywords: Doxorubicin, Simvastatin, Testis, Mouse |
|
Full-Text [PDF 823 kb]
(2362 Downloads)
|
Type of Study: Research |
Received: 2017/01/9 | Accepted: 2017/04/3 | Published: 2017/07/10
|
|
|
|