:: Volume 25, Issue 5 (1-2018) ::
Journal of Ilam University of Medical Sciences 2018, 25(5): 26-33 Back to browse issues page
Analyzing the Abundance of the Gene glmM in Subjects with a Positive Helicobacter pylori Stool Antigen Test (HPSA) and Correlating glmM Abundance with Serum Levels of Cytokines TNF-α and IL-1β
Ayat Moradipour1 , Afra Khosravi * 2, Mohammadreza Mehrabi3
1- Dept of Microbiology, Borujerd Branch, Islamic Azad University, Borujerd, Iran
2- Dept of Immunology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran , afrakhosravi@yahoo.co.uk
3- Dept of Laboratory Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran
Abstract:   (4574 Views)

Introduction: One of the most common genes in Helicobacter pylori is glmM which is usually used for identification of infection with the bacterium within gastric biopsy specimens by PCR, as a target gene. The aim of this study was to examine the prevalence rate of glmM gene in DNA samples of stool and evaluate its relationship with the fluctuations of serum levels of TNF-α and IL- 1β.
Materials & methods: In this study, blood and stool samples were collected from 82 subjects in two groups with positive and negative HPSA test referred to laboratories of Ilam, Iran. PCR was used to investigate the presence of glmM gene in DNA extracted from stool samples, and then serum levels of studied cytokines were measured using specific kits by ELISA, and the results were analyzed using statistical tests.
Findings: Analysis of data obtained from the study showed that the frequency of glmM gene in the sample of cases with positive HPSA was 23.8%, and there was a significant relationship between the presence of this gene in stool and fluctuations in serum levels of TNF-α and IL- 1β (P <0.05).
Disussion & conclusion: According to data, it can be said that the presence of glmM gene was associated with fluctuations in any of the variables of HPSA, TNF-α and IL- 1β and the possible presence of the gene in the stool raises with increase in each unit of HPSA and IL- 1β, which in turn raises the prognosis of diseases with a virulence form of bacteria by both serology and stool methods.

Keywords: Helicobacter pylori, glmM, TNF-α, IL- 1β, HPSA
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Type of Study: Research |
Received: 2016/01/25 | Accepted: 2016/06/6 | Published: 2018/01/6

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