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Determination of Antibacterial Activity of Pistacia lentiscus Methanolic Extract on Staphylococcus aureus, Streptococcus mutans, Streptococcus sanguis , Pseudomonas aeruginosa
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Noushin Jalayer Naderi *   1, Mohamad Niakan2 , Maryam Mohamadi Motlagh2 |
1- Shahed University , jalayer@shahed.ac.ir
2- Shahed University |
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Abstract: (9334 Views) |
| Introduction: There are different species of bacteria in different parts of oral c-avity that cause the tooth decay and pe-riodontal diseases. Pistacia lentiscus L. has been used for gingival problems, to-othache and aromatizing the mouth in Iranian traditional medicine. The aim of this study was evaluating the antibac-terial effect of Pistacia lentiscus L. on Staphylococcus aureus, Streptococcus mutans, Streptococcus sanguis and Ps-eudomonas aeruginosa. Materials & Methods: Methanolic ex-tract of Pistacia lentiscus L. from 100 to1000 mg/ml dilution were examined on strains of Staphylococcus aureus (ATCC25923), Streptococcus mutans( ATCC1601), Streptococcus sanguinis (ATCC1449) and Pseudomonas aerugi-nosa (ATCC25923 ). The well assay an-d disc diffusion methods were applied for testing the antibacterial activity of Pistacia lentiscus L. The Minimum Inh-ibitory Concentration (MIC) and Min-imum Bactericidal Concentration (MB-C) were detected. Findings: The Streptococcus mutans an-d Pseudomonas aeruginosa were not se-nsitive to Pistacia lentiscus L. Staphyl-ococcus aureus and Streptococcus sang-uinis were sensitive at 50, 100 and 1000 mg/ml concentrations. With disk diff-usion method, sensation to Pistacia lent-iscus L. was only showed at 100 mg/ml concentration for Streptococcus sangu-inis that produced an inhibition zone with 17 mm diameter. The MIC of Pist-acia lentiscus L. for Streptococcus sang-uinis was 1000 mg/ml. The other bacte-ria grew in all concentrations. Discussion & Conclusion: The meth-anolic extract of Pistacia lentiscus L. in some concentrations has antibacterial effect on Staphylococcus aureus and St-reptococcus sanguinis, but it doesn´t have any antibacterial effect on strept-ococcus mutans and Pseudomonas aeru-ginosa. |
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| Keywords: Antibacterial effect, oral cavity, Pistacia lentiscus L |
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Full-Text [PDF 487 kb]
(2742 Downloads)
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Type of Study: Research |
Subject:
Microbiology
Received: 2014/01/13 | Accepted: 2014/07/21 | Published: 2015/03/7
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