[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 21, Issue 2 (6-2013) ::
sjimu 2013, 21(2): 51-59 Back to browse issues page
In Vitro Studing of the Effect of Antibiotics on Planktonic Cells and Biofilm Clinical Isolates of Enterococcus
Mitra Salehi, Forough Abangah 1, Farzaneh Hosseini
1- , foroughabangah@yahoo.com
Abstract:   (12141 Views)

Abstract

IntroductionEnterococci are part of the normal flora intestinal tract of human and animal.In contrast to the beneficial role that Enterococci play in intestinal homeostasis,becoming important to human health as leading cause of nosocomial infections.The most important characteristics of Enterococci is the considerable amount of antibiotic resistance. Some strains of E.faecalis can form biofilm, which increase their ability to colonize patients and persist at infection sites.Bacteria in biofilms are generally showing more resistance to the antibiotics in planktonic condition. Material and method:In this study, strains Enterococci isolated from some patients following the determination of antibiotic resistance and measured amount MIC and MBC ,The most sensible strains were chose for the biofilm modeling invitro .The effect of antibiotic have been studied on biofilm. Finding:The most and the least antibiotic resistance of Enterococci strain was determined respectively to Penicillin (100%) and Nitrofurantoin (7%).Antibiotics Ampicilin,Gentamicin,Tetracycline even in densities 10times more than MIC of planktonic condition were disable in obliterating of the biofilm of strains.Different densities of Nitrofurantoin antibiotic collapsed bacterial biofilm absolutely.The least density 256 µg/ml was seen in one-day biofilm and the most density 1024 µg/ml,on the fifth day. Discussion and Conclusion: The results of bactericidal activities confirm the overt difference between planktonic cells and biofilm showing resistance of biofilm toward planktonic.According to the results obtained, it seems that Nitrofurantoin antibiotic is more effective for treatment of Enterococci in planktonic and biofilm condition.

Keywords: Key words: Enterococci, planktonic, biofilm, Nitrofurantoin
Full-Text [PDF 467 kb]   (2750 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2012/05/23 | Accepted: 2013/08/6 | Published: 2013/09/1
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

salehi M, abangah F, hosseini F. In Vitro Studing of the Effect of Antibiotics on Planktonic Cells and Biofilm Clinical Isolates of Enterococcus. sjimu. 2013; 21 (2) :51-59
URL: http://sjimu.medilam.ac.ir/article-1-616-en.html


Volume 21, Issue 2 (6-2013) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
Persian site map - English site map - Created in 0.19 seconds with 31 queries by YEKTAWEB 4241