:: Volume 28, Issue 3 (8-2020) ::
sjimu 2020, 28(3): 53-62 Back to browse issues page
Anticancer Effect of Heracleum persicum Alcoholic Extract and Leishmania Major Promastigute in Comparison with Doxorubicin in MCF7 Breast Cancer Cell Line and Natural HU02 Fibroblast
Zahra Bagheri-Hosseinabadi1 , Fatemeh Javani Jouni2 , Seyedeh Nahid Sajjadi3, Hossein Vazini 4, Ameneh Elikaei5
1- Dept of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
2- Dept of Biomedical Engineering, Faculty of Health, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
3- Dept of Biology, Islamic Azad University, Ayatollah Amoli Branch, Amol, Iran
4- Dept of Nursing, Faculty of Basic Sciences, Hamadan Branch, Islamic Azad University, Hamadan, Iran , hossein_vazini@yahoo.com
5- Dept of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
Abstract:   (230 Views)
Introduction: Breast cancer is the most common cancer among women. Doxorubicin is one of the most important chemotherapy drugs in the treatment of tumors with many side effects. Today, herbal compounds make up about one-third of the total available drugs. Heracleum persicum has anti-cancer effects. Moreover, in recent years, there has been an interest in the study of new drug targets, such as the use of parasitic compounds in cancer treatment. Therefore, this study aimed to investigate the anticancer effects of Heracleum persicum alcoholic extract and Leishmania major promastigute in comparison with doxorubicin in MCF7 breast cancer cell line and HU02 fibroblast.
Materials & Methods: Breast and fibroblast cell lines were exposed to logarithmic concentrations of doxorubicin, Heracleum persicum extract, and Leishmania promastigute separately and in combination. The MTT was used to determine the inhibitory concentration of this herb extract, medicine, and the parasite. The data were analyzed in SPSS software. Ethics code: IR.SKUMS.0063-99
Findings: The results showed that an increase in the concentration of doxorubicin, Heracleum persicum extract, and Leishmania promastigute individually and in combination led to a decrease in the percentage of live fibroblast cells and MCF7. The highest percentage of the decreased live cells is observed in the combined application of Heracleum persicum extract, Leishmania promastigute, and doxorubicin.
Discussion & Conclusions: Heracleum persicum extract and Leishmania promastigute can have significant inhibitory effects on the percentage of live fibroblast cells and MCF7, which is more pronounced in living cells of MCF7.
Keywords: Breast Cancer|Doxorubicin|Heracleum persicum alcoholic Extract|Leishmania Major promastigute ,
Full-Text [PDF 677 kb]   (73 Downloads)    
Type of Study: Research | Subject: medical parasitology
Received: 2019/05/7 | Accepted: 2020/01/7 | Published: 2020/08/31

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Volume 28, Issue 3 (8-2020) Back to browse issues page