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Human IgG Class And Subclass Against Crude Hydatid
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E Baba Ahmadi   , M Shamsi , A Khosravi * , K Sayehmiri , K Saki |
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Abstract: (23474 Views) |
| Abstract
Introduction: Application of crude hydatid (HCF) cyst fluid antigen for the diagnosis of cystic hydatid (CH) is a method which along with immediate serological investigation can be helpful and effective in rapid treatment of the disease. However, there is no standard, highly sensitive, and specific serological test for antibody detection in cases of human CE yet. The current study aimed to evaluate the cross- reactivity of human IgG against human crude hydatid fluid antigens compared to B fraction of sheep cystic fluid antigen in order to find the target antigens with the highest IgG class, IgG subclass.
Materials & Methods: This is an analytical case-control study using human crude HCF as the source of antigen for performing ELISA and Western blotting. Sample sera used in present work were collected from patients who recently had hydatid surgery in hospitals of Tehran, Hamadan and Ilam cities as human case group together with some human or animal sera with no history of hydatidosis with negative HCF using ELISA and IFAT as control group. Briefly, the required antigen was extracted and prepared from human hydatid fluid cysts. 30 positive samples sera from human sources were used as the case together with 30 healthy sera as control group. Hydatid cyst fluid antigen preparation was carried out according to the procedure described by Mamuti, et al, with slight modifications. ELISA method was carried out as described by Verastegui.
Findings: The highest mean OD value in response to human HCF antigen was related to IgG4, while the lowest to IgG3. The sensitivity and specificity of ELISA test used for evaluating the responses of human total IgG to HCF antigen were 100 and 95.8% respectively. Cross-reaction of human IgG class and subclasses responses was found for both antigens with the best reaction against human HCF antigen compared to antigen B using a ratio of mean OD value to each antigen divided by the cut-off point value for the same antigen.
Discussion & Conclusion: Human sera showed a considerable cross-reactivity against human HCF antigens and antigen B by ELISA test. The best human IgG subclass response against all antigens was found to be IgG4. |
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| Keywords: hydatid cyst, crude antigen, antibodies, ELISA |
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Full-Text [PDF 238 kb]
(2424 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2011/12/11 | Published: 2011/04/15
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