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:: Volume 26, Issue 6 (3-2019) ::
sjimu 2019, 26(6): 133-142 Back to browse issues page
Investigating the Cytotoxic Effect and Antioxidant Properties of Green Synthesized Silver Nanoparticles from the Root of Persicaria Bistorta on Human Liver Cancer Cell Line (Hep G2)
Reyhaneh Shali1 , Ali Neamati Dr 2, Pouran Ardalan Dr3
1- Dept of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2- Dept of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran , neamati.ali@gmail.com
3- Dept of Chemistry, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Abstract:   (3219 Views)
Introduction: The use of nanoparticles to inhibit the growth and proliferation of cancer cells is one of the achievements of the nanotechnology science. Studies have shown that silver nanoparticles have cytotoxic effects on the growth of cancerous cells. On the other hand, the antioxidant properties of this nanoparticle have been proven in many studies. Therefore, in this investigation, antioxidant capacity and cytotoxicity effects of silver nanoparticles synthesized from Root of Persicaria bistorta L (Bi/Ag-NPs) on liver cancer cells (HepG2) compared with normal skin cells (HDF) were studied. In addition, ABTS and DPPH free radical scavenging capacity of Bi/Ag-NPs was evaluated using colorimetric tests.
 
Materials & Methods: In order to evaluate the cytotoxicity effects, first the cancerous and normal cells were cultured and treated with various concentrations of Bi/Ag-NPs and finally, the cell survival rate was estimated using the MTT assay. The antioxidant activity of Bi/Ag-NPs was evaluated according to the amount of ABTS and DPPH free radicals inhibition.
 
Findings:  The results showed that Bi/Ag-NPs inhibited cancer cells with an IC50 value of about 3 μg/ml, while at this concentration they were ineffective on normal cells (IC50: 50 μg/ml). The scavenging of ABTS (IC50: 15 μg / ml) and DPPH (IC50: 20 μg/ml) free radicals confirmed the antioxidant properties of Bi/Ag-NPs.
 
Discussion & Conclusions: The cytotoxic and antioxidant results show that Bi/Ag-NPs can be used as auxiliary agents for treating many diseases that are caused by oxidative stress and other biomedical applications.    
Keywords: Antioxidant, Silver nanoparticles, Persicaria bistorta, Cancer
Full-Text [PDF 684 kb]   (1031 Downloads)    
Type of Study: Research |
Received: 2017/11/22 | Accepted: 2018/02/5
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Shali R, Neamati A, Ardalan P. Investigating the Cytotoxic Effect and Antioxidant Properties of Green Synthesized Silver Nanoparticles from the Root of Persicaria Bistorta on Human Liver Cancer Cell Line (Hep G2) . sjimu. 2019; 26 (6) :133-142
URL: http://sjimu.medilam.ac.ir/article-1-4671-en.html


Volume 26, Issue 6 (3-2019) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
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