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:: Volume 26, Issue 3 (9-2018) ::
sjimu 2018, 26(3): 187-199 Back to browse issues page
A study on separated and combined effects of stoechas extract(Lavandula stoechas) and silver nano-particles on Klebsiella pneumoniae biofilm formation
Reza Habibipour 1, Leila Moradi Haghgou2
1- Dept of Microbiology, Faculty of Basic Science, Hamedan Branch, Islamic Azad University, Hamedan, Iran , habiby.reza@gmail.com
2- Dept of Microbiology, Faculty of Basic Science, Hamedan Branch, Islamic Azad University, Hamedan, Iran
Abstract:   (2336 Views)

Introduction: So-called biofilm or biomass is interacting cells or microorganisms that accumulate together as a result of various factors on a surface. Biofilm formation increases resistance to unfavorable conditions and biocides. Of the strategies to deal with biofilms is using different plant compounds and nanoparticles. The aim of this study was to determine the effect of silver nanoparticles, lavender extract, and their combined effects in different concentrations on Klebsiella pneumoniae biofilm.
 
Materials & Methods: The anti-biofilm effect and reduced rate of biofilm formation of Klebsiella pneumoniae in separated and combined treatments of stoechas extract (Lavandula stoechas) and silver-nanoparticles at 24, 48 and 72 hours were measured by colorimetric microtiter method. Phenolic content was also measured by Folin-Ciocalteu method.
 
Findings: Phenolic content was about 113.3
mg/g. In the absence of treatments, Klebsiella was able to form strong biofilms. Three-way-analysis of variance of the main and interacting effects of silver nanoparticles, lavender extract and time on biofilm formation was significant. Silver nanoparticle and lavander extract at concentrations of 125 and 0.0625 ug/mL, respectively, and co-treatments at 62.5 and 0.03125 ug/mL concentrations, respectively, significantely reduced biofilm formation.
 
Discussion & Conclusions: By increasing time, biofilm formation reduced due to effects of treatments. Biofilm formation also reduced by increasing concentration of the treatments. The result of this survey showed that silver-nanoparticles and lavender extract have synergistic effects on reduction of biofilm formation.

Keywords: Biofilm, Silver nano-particle, Lavander extract, Klebsiella pneumoniae, ELISA
Full-Text [PDF 892 kb]   (655 Downloads)    
Type of Study: Research |
Received: 2017/02/4 | Accepted: 2017/07/19 | Published: 2018/09/15
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Habibipour R, Moradi Haghgou L. A study on separated and combined effects of stoechas extract(Lavandula stoechas) and silver nano-particles on Klebsiella pneumoniae biofilm formation . sjimu. 2018; 26 (3) :187-199
URL: http://sjimu.medilam.ac.ir/article-1-4129-en.html


Volume 26, Issue 3 (9-2018) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
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