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:: Volume 25, Issue 4 (11-2017) ::
sjimu 2017, 25(4): 108-116 Back to browse issues page
Identification of Drug-Repellent Encoded Genes (Efflux pump) in Pseudomonas aeruginosa Isolated from Stool Samples, Kerman Province
Maryam Zarenia Mrs1, Mitra Salehi Dr 2, Babak Kheirkhah Dr3
1- Dept of Microbiology, Islamic Azad University, Sirjan Branch, Sirjan, Iran
2- Dept of Microbiology, Islamic Azad University, North Tehran Branch, Tehran, Iran , alm3370@yahoo.com
3- Dept of Microbiology, Islamic Azad University, Kerman Branch, Kerman, Iran
Abstract:   (1936 Views)

Introduction: Pseudomonas aeruginosa is one of the important bacteria for nosocomial infections; particularly in patients with immune deficiency. For the treatment of serious infections caused by these bacteria, antibiotics such as aminoglycosides many, quinolones and beta-lactams are used. However the emergence and spread of resistant nosocomial strains of resistance have been reported. Nowadays, efflux pump has been proposed as an important mechanism of resistance to antibiotics in bacteria.
 
Materials & methods: In this cross-sectional study, 60 clinical isolates of P.aeruginosa were collected from fecal samples from laboratories in Kerman province. All isolates were approved with the phenotype and biochemical tests. The disk diffusion method was performed according to CLSI (Clinical and Laboratory Standards Institute). Multiplex-PCR tests were performed for detection of efflux pumps genes (mexA, mexB, mexR) using specific primers.
 
Findings: All the 60 fecal isolates of P..aeruginosa were confirmed by biochemical tests. The greatest sensitivity was observed for ceftizoxime 55(91.7%), imipenem, 54(90%), meropenem, 48(80%) and ciprofloxacin 40(66.7%). The greatest resistance were determined to antibiotics cefepime 36(60%) and ciprofloxacin 19(31.7%). The highest frequency was observed of Mex B gene in 59(98.3%) of the isolates.
 
Discussion & conclusions: Rapid diagnosis of Pseudomonas aeruginosa in clinical samples is more than important for initiation of treatment. In general, the use of multiplex polymerase chain reaction for detection of antibiotic-resistant P. aeruginosa is very important in clinical samples of patients.
 

Keywords: Pseudomonas aeruginosa, Efflux pump genes, Multiplex PCR
Full-Text [PDF 847 kb]   (1022 Downloads)    
Type of Study: Research |
Received: 2016/01/14 | Accepted: 2016/06/26 | Published: 2017/11/22
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Zarenia M, Salehi M, Kheirkhah B. Identification of Drug-Repellent Encoded Genes (Efflux pump) in Pseudomonas aeruginosa Isolated from Stool Samples, Kerman Province. sjimu. 2017; 25 (4) :108-116
URL: http://sjimu.medilam.ac.ir/article-1-3311-en.html


Volume 25, Issue 4 (11-2017) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
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