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Comparison of Antibody Titers against the Single, Mixed, Fused and Recombinant proteins, StxB, IpaD and StxB- IpaD
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Abstract: (7488 Views) |
| Introduction: IpaD protein plays an im-portant role in invasion, pathogenesis and infection caused by Shigella and E. coli. Another major virulence factor in Shigella dysenteriae type 1 and E. coli O157: H7 is Shigella entrotoxin or (StxB). A suitable candidate vaccine could be made by a mixture or fusion of IpaD and STxB pr-oteins. In this study, the immunogenicity and antibody titer of fused mixed and separated recombinant proteins, IpaD and STxB, in mice were compared with each other. Materials & Methods: In this experimental study, recombinant vectors containing the gene sequences stxB-IpaD, IpaD and stxB, were prepared and also transferred into the E. coli BL21 DE3 bacterium. The protein expression levels were assessed and their expression was approved by using of We-stern blot technique. After purification of recombinant proteins with the column of chromatography, they were injected four times consecutively to the mice. Findings: The antibody titers were statisti-cally different for STxB, IpaD, STxB-IpaD and IpaD mixed with STxB antigens in the mice, and antibody levels increased by adding the StxB to IpaD. Discussion & Conclusion: The results of this study indicate that the furin linker sep-arated by semi furins and the produced pro-tein from mixture and fusion genes, ipaD and stxB, can increase STxB immunege-nicity effect, and could be a good candidate for the production of recombinant vaccines against Shigella and E. coli types. |
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| Keywords: Shigella dysenteriae type 1, B subunit of Shiga toxin (STxB), IpaD, E. coli O157: H7 |
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Full-Text [DOCX 1102 kb]
(4107 Downloads)
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Type of Study: Research |
Subject:
anesthesia
Received: 2015/03/7 | Accepted: 2015/03/7 | Published: 2015/03/7
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