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:: Volume 23, Issue 3 (9-2015) ::
sjimu 2015, 23(3): 168-178 Back to browse issues page
Investigating the Cytotoxic Effects of a New Synthetic Chalcone Derivative on HeLa Cell Line
Bagher Seyedalipour 1, Yeganeh Taghavi2, Mohammad A Khalilzadeh2
1- Mazandaran University , b.alipour81@gmail.com
2- Islamic Azad University, Qaemshahr Branch
Abstract:   (5491 Views)

Introduction: Chalcones and their derivatives have strong anti-cancer activity. The aim of present study was to evaluate the cytotoxicity effect of new synthetic chalcone on Hela cell line.
 Material &methods: Hela cell line were cultured in RPMI1640 liquid medium along with with 10% inactivated fetal bovine serum (FBS) and antibiotics. Cells were cultured with various concentrations of chalcone for 72 hours and were analyzed by using the MTT test. Statistical analysis was performed by using of students T-Test. Apoptosis were examined on Hela cell lines after 24 hours in concentration of 40 µg/ml chalcone by Microscopic method.
 Findings: The results of this study showed that new synthetic chalcone at concentrations of (10, 20, 40, 60, 80, 160 µg/ml) inhibit the growth of Hela cell line 63.75 ،56.58،54.46،33.60،18.74،14.96 after 72 hours, respectively. The highest percentage of growth inhibition at a concentration of 10 µg/ml was 63.753. The IC50 value for Hela cells was 40 μg/ml. Our findings showed that grow the cells have reduced at concentrations of 10 and 20 µg/ml significantly compared to control group after 72 hours.
 Discussion & Conclusion: Based on our research, synthetic chalcone has anti proliferation activity against HeLa cells lines. This activity is related to its effect on cell cycle by inducing G2 / M phase. Further studies are needed to demonstrate the molecular mechanisms of this new chalcone

Keywords: Chalcone, Cytotoxicity, HeLa cell lines, MTT test
Full-Text [PDF 821 kb]   (2044 Downloads)    
Type of Study: Research | Subject: biology
Received: 2014/06/29 | Accepted: 2014/09/7 | Published: 2015/09/29
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Seyedalipour B, Taghavi Y, Khalilzadeh M A. Investigating the Cytotoxic Effects of a New Synthetic Chalcone Derivative on HeLa Cell Line. sjimu. 2015; 23 (3) :168-178
URL: http://sjimu.medilam.ac.ir/article-1-1924-en.html

Volume 23, Issue 3 (9-2015) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
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