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:: Volume 30, Issue 4 (10-2022) ::
Journal of Ilam University of Medical Sciences 2022, 30(4): 106-116 Back to browse issues page
Association of Exon 1 Variants of NOEY2 Gene with Poly cystic Ovary Syndrome (PCOS) and Diabetes
Parichehr Karajibani1 , Abolhasan Rezaei * 2
1- Dept of Genetics, Faculty of Basic Science, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran
2- Dept of Genetics, Faculty of Basic Science, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran , a.rezaei7300@gmail.com
Abstract:   (1367 Views)
Introduction: Polycystic ovary syndrome (PCOS) is the cause of 75% of infertility due to lack of ovulation. This study aimed to investigate the possible association between exon 1 polymorphisms of the NOEY2 gene in women with PCOS and diabetes.
Material & Methods: This study was conducted on 240 blood samples (1 cc each) from women in four groups: polycystic, diabetes, polycystic-diabetes, and control. Genomic DNA extraction was performed using the extraction kit, according to the protocol. A primer pair was designed and synthesized for the exon 1 sequence of the NOEY2 gene. After gene amplification by polymerase chain reaction (PCR) and evaluation of band quality, Sanger sequencing was performed on PCR products. Sequencing results were analyzed using Chromas, GeneRunner, and Alignment software, and statistical calculations were performed by SNPAlyze, SPSS, and Web-Assotest program.
(Ethic code: IR.IAU.TON.REC.137031)
Findings: Sequence analysis confirmed two polymorphisms: 5'UTR c.156G> A polymorphism was observed in exon 1 of NOEY2 and second polymorphism of 5'UTR c.207+76 G>A, 76 nucleotides farther from exon 1 and in the intron region of NOEY2 gene. Statistical analysis showed a statistically significant difference between 5'UTR c.156G> A and susceptibility to PCOS and diabetes.
Discussion & Conclusion: No report on this region was available in genetic databases and this polymorphism was reported for the first time in the present study. Bioinformatics studies showed that this polymorphism can affect the affinity to bind in certain transcription factors by allelic changes due to its location in the CTCF overlapping promoter region.
Keywords: Diabetes, Polymorphism, NOEY2 gene, Polycystic ovary syndrome
Full-Text [PDF 1347 kb]   (713 Downloads)    
Type of Study: Research | Subject: Molecular Genetics
Received: 2021/04/7 | Accepted: 2022/04/5 | Published: 2022/10/7
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Karajibani P, Rezaei A. Association of Exon 1 Variants of NOEY2 Gene with Poly cystic Ovary Syndrome (PCOS) and Diabetes. J. Ilam Uni. Med. Sci. 2022; 30 (4) :106-116
URL: http://sjimu.medilam.ac.ir/article-1-7052-en.html
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Volume 30, Issue 4 (10-2022) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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