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:: Volume 27, Issue 2 (6-2019) ::
sjimu 2019, 27(2): 73-82 Back to browse issues page
Identification of Biofilm Encoding Genes (agg) in the Escherichia coli Isolates by Multiplex-PCR Method
Razieh Jafari1 , Kumarss Amini 2, Behroz Shojaie Sadi3
1- Dept of Microbiology, Sirjan Branch, Islamic Azad University, Sirjan, Iran
2- Dept of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran , dr_kumarss_amini@yahoo.com
3- Dept of Microbiology, Arak Branch, Islamic Azad University, Arak, Iran
Abstract:   (145 Views)
Introduction: Biofilm is a collection of microbial cells that are irreversibly suppressed and mildly washed away. The aggR gene is located on the main plasmid (pAA), which codes for many actuator factors. The pathogen strains of EAEC have an aggR gene, the gene has been identified in six classes of pathogenic E. coli.
 
Materials & Methods: In the present study, 60 stool samples from children with an average age of 4 to 7 years were collected and after biochemical examinations such as catalase, oxidase, and TSI environment were identified. Ethics code: IR.IAUS.REC.1397.016
 
Finding: The aggA gene was in 7 samples 11.6%, agg4A 9 samples 15%, aggR 5 positive 8%. It was also found that 28% of isolated isolates had at least one of the factors of aggressiveness, aggR, aggA, and agg4A.
 
Discussion & Conclusions: The typical EAEC strains have a high prevalence of aggR genes in isolates isolated in this region and are heterozygous according to the pattern of virulence factors. By limiting the infections of this bacterium, it is a vaccine against a strain of the pathogen.
 
 
Keywords: Escherichia coli, Multiplex- PCR, AggR, AggA, Biofilm
Full-Text [PDF 967 kb]   (45 Downloads)    
Type of Study: Research |
Received: 2018/01/13 | Accepted: 2019/02/2
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Jafari R, Amini K, Shojaie Sadi B. Identification of Biofilm Encoding Genes (agg) in the Escherichia coli Isolates by Multiplex-PCR Method . sjimu. 2019; 27 (2) :73-82
URL: http://sjimu.medilam.ac.ir/article-1-4781-en.html


Volume 27, Issue 2 (6-2019) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
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