[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 27, Issue 1 (4-2019) ::
sjimu 2019, 27(1): 64-74 Back to browse issues page
Effect of Lactobacillus Casei on Hematological and Histopathological Factors in Rats Infected with Salmonella typhimurium
Mahnoosh Fatemi1 , Fereshte Ghandehari 2, Atefeh Kheirkhah3
1- Dept of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2- Dept of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran , ghandehari@iaufala.ac.ir
3- Dept of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Abstract:   (542 Views)
Introduction: Salmonella typhimurium is one of the most important causes of gastrointestinal diseases in humans and animals. Probiotics inhibit pathogens, such as Salmonella Typhimurium by producing antimicrobial compounds. Therefore, this study assessed the protective effect of Lactobacillus casei on hematological factors in rats infected with Salmonella typhimurium with the possibility of salmonellosis treatment.
 
 Materials & Methods: Totally, 32 male rats (150±20 g) were purchased and randomly divided into four groups, namely injection control group (A), infected with Salmonella typhimurium (1.5×108 CFU/ml) (B), treated with Lactobacillus casei (C), and infected with Salmonella typhimurium and treated with Lactobacillus casei (D). After the treatment, the blood sample was taken in order to evaluate blood parameters. In addition, a part of the liver tissue was separated and stained with hematoxylin-eosin. The other part was homogenized and cultured based on pour plate method. Subsequently, the number of pathogen colonies was counted in tissues.
 
Findings: According to the results, the number of lymphocytes, monocytes, and basophiles had increased significantly in group B than those in the control group. Histopathological changes and cell death were evident in the livers of the group B. However; the liver tissues of other groups had normal appearance. In addition, the growth of Salmonella Typhimurium bacteria was significantly lower in group D, compared to group B.
 
Discussion & Conclusions: The results showed that Lactobacillus casei could overcome Salmonella Typhimurium without any adverse effects on hematological and histopathological factors and it could prevent the development of disease to some extent.
 
Keywords: Hematology, Histopathology, Lactobacillus Casei, Salmonella typhimurium
Full-Text [PDF 944 kb]   (155 Downloads)    
Type of Study: Research |
Received: 2017/10/25 | Accepted: 2018/04/3
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Fatemi M, Ghandehari F, Kheirkhah A. Effect of Lactobacillus Casei on Hematological and Histopathological Factors in Rats Infected with Salmonella typhimurium . sjimu. 2019; 27 (1) :64-74
URL: http://sjimu.medilam.ac.ir/article-1-4602-en.html


Volume 27, Issue 1 (4-2019) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
Persian site map - English site map - Created in 0.18 seconds with 32 queries by YEKTAWEB 3986