96 1563-4728 Ilam University of Medical Sciences 4282 biology Antioxidant Activity of Glycyrrhiza glabra L. Extract and Protective Effect of its Leaf Extract on Ethanol-Induced Nephrotoxicity in Male Rats Karamian Roya b Asadbeigy Mostafa c Yari Siamak d b Dept of Biology, Faculty of Science, Bu-Ali Sina University, Hamedan, Iran c Dept of Biology, Faculty of Science, Bu-Ali Sina University, Hamedan, Iran d Dept of Biology, Faculty of Science, Bu-Ali Sina University, Hamedan, Iran 1 11 2018 26 4 1 12 28 04 2017 08 07 2017 Introduction: Acute alcohol consumption leads to induction of lipid peroxidation in renal tissues, but its chronic consumption has moderate effects on biochemical and histological characteristics of this organ. Antioxidants have protective effects against ethanol-induced oxidative stress and tissue injury. The aim of this study was to assess antioxidant activity of Glycyrrhiza glabra leaf and stem extracts and the protective effect of its leaf extract on ethanol-induced nephrotoxicity.   Materials & methods: Total phenol and flavonoid contents of leaf and stem extracts of G. glabra were measured by Folin Ciocalteu and AlCl3 assays, respectively. Antioxidant activity of both extracts was assessed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. In addition, protective effect of the leaf extract was assessed using biochemical and histological analyses of renal tissues of male Wistar rats, which were divided into four groups including group 1 or control (received 1 ml distilled water daily), group 2 or ethanol group (received 1 ml of 50% ethanol daily), group 3 or ethanol + leaf extract group (received 1 ml of 50% ethanol + 500 mg/kg leaf extract daily), and group 4 (received 500 mg/kg of leaf extract daily). All treatments are performed through intragastric administration. Biochemical and histological analyses were used for the evaluation of nephrotoxicity. For histological study, the samples were stained with Hematoxylin-Eosin and examined by light microscopy. Finally, all the data were analyzed by SPSS (Ver. 20) and grouped by Duncan's Multiple Range Test at P <0.05 level.   Findings: There was no significant difference between total phenol contents of the stem and leaf extracts. However, the stem extract showed a higher total flavonoid content than the leaf extract. Also, both the extracts showed higher antioxidant activities (86-93%) than that of ascorbic acid (71%). Results from biochemical analysis indicated a significant increase in superoxide dismutase (SOD) activity and H2O2 content in the renal tissues of ethanol-treated rats in comparison with other groups; however, there were no significant changes in total protein and malondialdehyde (MDA) contents. Results from histological examination showed that alcohol consumption intensity injured kidney tissues, which was effectively moderated by the studied extract.   Discussion & Conclusions: Results from the present study showed that G. glabra extract has biological activity and can be used in future as a new natural antioxidant in food and drug industries.
4103 General Accuracy Rate of the Demographic Data Registered for Patients with Thyroid Cancer in Isfahan University of Medical Sciences: A Cross-sectional Study Based on Cancer Registry Data of 2010-2016 1 11 2018 26 4 13 22 21 01 2017 08 07 2017 Introduction: Thyroid cancer has always been considered as the most common endocrine malignancy. Women in Isfahan Province were ranked first in thyroid cancer in Iran in 2009. Therefore, this study aimed to evaluate the accuracy of the demographic data registered for women with thyroid cancer in Isfahan University of Medical Sciences during 2006-2010. Materials & methods: This cross-sectional study was based on registered data. Data collection was performed using output of cancer registry software that contains information including type of cancer and patients' demographic characteristics in the form of an Excel file. Data were analyzed by using frequency distribution table, percentage and mean, as well as z-test to compare proportions.   Findings: The accuracy rates of registration of age, name, surname, father's name, province and country of residence of the patients were 82.28%, 99.31%, 98.66%, 98.47%, 95.74%, and 93.87%, respectively. In terms of accuracy of registration of name (P = 0.044), surname (P = 0.0046), age, father’s name, province, and country of residence (P <0.001), there were significant differences at the 5% level of error with the full coverage of registration accuracy index.   Discussion & conclusions: The accuracy of the recorded information for women with thyroid cancer was acceptable in comparison with similar studies and national average. Considering that burden of cancer over the next few years is predicted to increase, high standards in cancer registry program should be maintained by boosting hardware and software resources for patients and staff as well as continuing education.   4148 Microbiology Study the Antibacterial Effects of Alcoholic Extract of Pelargonium Sp on the Growth of three Bacteria: Pseudomonas aeruginosa, Enterococcus faecalis and Multidrug-Resistant E.coli Morris Sanaz h Momen Amir Hossein i Yousefi mahmoud Nafiseh j h Dept of Microbiology, Faculty of Science, Hamedan Branch, Islamic Azad University, Hamedan, Iran i Dept of Microbiology, Faculty of Science, Hamedan Branch, Islamic Azad University, Hamedan, Iran j Dept of Microbiology, Faculty of Science, Hamedan Branch, Islamic Azad University, Hamedan, Iran 1 11 2018 26 4 23 33 12 02 2017 17 05 2017 Introduction: According to the increasing resistance of pathogenic bacteria against antibiotics, searching to find new alternatives to chemical drugs and antibiotics has recently become popular. Therapeutic and pharmaceutical effects of medicinal plants have been considered for decades. The aim of this study was to investigate the effect of alcoholic extract of Geranium plant on the growth of antibiotic resistant bacteria that are prevalent in hospitals.   Materials & methods: To investigate the antimicrobial activity of Geranium, clinical and standard strains of Pseudomonas aeruginosa and Escherichia coli bacteria and standard strain of Enterococcus faecalis were treated by 25 and 50 mg/ml concentrations of alcoholic extract of Geranium. For this purpose, disk diffusion method was employed. In order to measure minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), micro broth dilution method was applied.   Findings:  The most sensitive bacterium was Pseudomonas aeruginosa due to showing the highest diameter of the inhibition zone (24 mm). The results showed that the alcoholic extract of Geranium plant at a concentration of 50 mg/ml had a higher antimicrobial effect.   Discussion & conclusions: The results showed that the alcoholic extract of Geranium plant has anti-bacterial properties. According to being indigenous and having therapeutic effects, further are is recommended to identify the therapeutic effects.  4308 pharmacology The Antidepressant-Like Effect of the Ethanolic Extract of Mentha Piperita in Forced Swimming Test and Tail Suspension Test in Male Mice Abbasi Maleki Saeid k Bakhtiarian Azam l Nikoui Vahid m k Dept of Pharmacology and Toxicology, Urmia Branch, Islamic Azad University, Urmia, Iran l Dept of Pharmacology, Tehran University of Medical Sciences, Tehran, Iran m Razi Drug Research Centers, Iran University of Medical Sciences, Tehran, Iran 1 11 2018 26 4 34 42 12 05 2017 09 07 2017 Introduction: Previous studies have revealed analgesic, anti-inflammatory, and antioxidant properties of Mentha piperita (MP). Hence, in this study, the antidepressant effects of the ethanolic extract of MP in forced swim test (FST) and tail suspension test (TST) in male mice were investigated.   Materials & Methods: In this experimental study, 96 male mice were randomly divided into 12 groups of 8 that received normal saline (10 ml/kg), imipramine (30 mg/kg), fluoxetine (20 mg/kg), and different doses of MP (100, 200 and 400 mg/kg), respectively. In FST, immobility time, swimming time, and climbing time and immobility time in TST were recorded during 6 minutes. In this study, all the drugs and extracts were injected intraperitoneally (i.p.) at the constant volume of 10 ml/kg.   Findings: Results shows that 200 and 400 mg/kg of the extract, as well as fluoxetine and imipramine reduced immobility time compared to the control group in FST and TST (p<0.001). In addition, the ethanolic extract and fluoxetine increased swimming time (p<0.001) without any significant change in climbing time (p>0.05). In contrast, imipramine increased climbing time without any significant change in swimming time (p>0.05).   Discussion & Conclusions: This extract like serotonergic agents (e.g., fluoxetine) decreases immobility time and increases swimming time without any significant change in climbing time. Hence, MP compounds (especially menthol) induced their effects through serotonergic mechanism. However, further studies are needed to clarify their exact mechanism of action. 4022 exclusive bacteriology The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa Naserian Moghadam Rahele n Roodbari Fatemeh o Naderi Nasab Mahboubeh p Mansouri Davoud Mirbagheri Seyedeh Zohre Gholoobi Aida Hooshyar chichaklu Amin Meshkat Zahra n Mazandaran University o Dept of Cell and Molecular Biology, Faculty of Basic Sciences, Mazandaran University, Mazandaran, Iran p Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Dept of Microbiology, Tehran University of Medical Sciences, Tehran, Iran Dept of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran 1 11 2018 26 4 43 52 24 12 2016 04 04 2017 Introduction: Antibiotic resistance crisis has always been a serious problem for human health and many hospitalized patients are affected worldwide. Pseudomonas aeruginosa is a gram-negative pathogen and one of the most common causes of nosocomial infections. The main mechanism of resistance to beta-lactam antibiotics is the presence of metallo-beta-lactamase (MBL) enzymes. Most of the MBL genes are found in plasmids. The aim of this study was to evaluate the frequency of MBL-producing P. aeruginosa isolates caused by VIM-all and VIM 2, 3, 9, 11and16 genes.   Materials & Methods: Antimicrobial susceptibility of 127 clinical isolates of P. aeruginosa was determined using the standard Kirby-Bauer disk diffusion method according to Clinical and Laboratory Standard Institute (CLSI). Combined-disk test was used for phenotypic determination of MBLs-producing isolates. After DNA extraction, VIM-all and in specific, VIM 2, 3, 9, 11 and 16 genes were amplified using PCR method.   Findings: A total Of 127 clinical isolates of P. aeruginosa, 62 isolates (49%) were resistant to imipenem and 31 isolates (24.5%) showed phenotypic evidences of MBL production. Moreover, among imipenem resistant strains VIM-all genes were found in 12.5% of cases, but the VIM 2-3-9-11 and 16 genes were not detected in samples.   Discussion & Conclusions: The results obtained in this study suggest that in P. aeruginosa, the highest antibiotic resistance observed was to cefazolin (98%) followed by nalidixic acid (91%) and the least resistance were to ciprofloxacin (31%). One of the reasons for this trend is the growth of antibiotic-resistant bacteria and the known mechanisms of bacterial resistance. 3737 Health Information management Quality Assessment of Ilam University of Medical Sciences Website from the Users’ Viewpoints According to WebQual Model Raeesi Saeed Darabi Maryam Dept of Statistics and Information Technology, Ilam University of Medical Sciences, Ilam, Iran Dept of Statistics and Information Technology, Ilam University of Medical Sciences, Ilam, Iran 1 11 2018 26 4 53 63 10 08 2016 10 08 2016 Introduction: Evaluation of quality of web pages from the users’ viewpoints is useful in its design and development. This study was performed to evaluate of the quality of Ilam University of Medical Sciences website from the users’ viewpoints.   Materials & Methods: The statistical population of this descriptive analytical study included 1300 staff of the university in 2014. Overall, 297 volunteers are selected by using Cochran’s formula and random sampling method. Data collection was performed with the WebQual checklist. To analyze the data, descriptive statistics (frequency, percentage, mean and standard deviation) and inferential statistics (one-sample t-test) were used in SPSS, version 16. Findings: According to the findings, the mean scores of benefit, ease of use, attractiveness, and interactiveness were 3.35, 3.79, 3.26, and 3.36, respectively, which are significantly different from 3 considered as the benchmark.   Discussion & Conclusions: The results showed that Ilam University of Medical Sciences website is in a good condition with respect to benefit, easy to use, attractiveness, and interaction. However, website administrators can further enhance the quality of the website in all these dimensions. 4067 midwifery Effect of Peer Support on the Incidence of Pregnancy during Breastfeeding in Nulliparous Women Karamelahi Zolaykha Tork zahrani Shahnaz Akbarzade baghban Alireza Chehreh Hashemieh Mohammad hasan nahal Monireh Dept of Midwifery, Faculty of Nursing and Midwifery, Ilam University of Medical Sciences, Ilam, Iran Dept of Midwifery, Faculty of Nursing and Midwifery, Shaheed Beheshti University of Medical Sciences, Tehran, Iran Dept of Biostatistics, Faculty of Allied Medical Sciences, Shaheed Beheshti University of Medical Sciences, Tehran, Iran Student Research Commitee, Faculty of Nursing and Midwifery, Shaheed Beheshti University of Medical Sciences, Tehran, Iran Dept of Midwifery, Faculty of Nursing and Midwifery, Ilam University of Medical Sciences, Ilam, Iran 1 11 2018 26 4 64 73 10 01 2017 16 07 2017 Introduction: Consecutive pregnancies with an of interval less than two years is associated with adverse consequences for the mother and child. Education and support of women during breastfeeding is one of strategies that have been used for appropriate spacing between pregnancies. This study aimed to investigate the effect of peer support on the incidence of pregnancy during breastfeeding and the use of family planning methods in Ilam, Iran.   Materials & Methods: This interventional study was conducted in birth and health centers of Ilam between 2010 and 2012 among 240 nulliparous women who were randomly divided into case and control groups. The control group received the standard educational programs, while the case group received both the standard education and peer support from 30 consultants for six months after delivery. One hundred fifty-four participants (79 subjects in the case group and 74 in the control group) completed the questionnaires.. The collected data were analyzed using decriptive statistics such as mean and percentage and chi-square test and Fisher's exact test in SPSS, version 16.   Findings: The results showed that 24 months after delivery,  pregnancy rate during breastfeeding in the control group was significantly higher than the case group (20% v.s  1.5%; P=0.004). The time of pregnancy in the control group was significantly earlier than the case group(9.6±3.56 months postpartum vs. 18.26±1.25; p=0.01). The two groups were statistically different in terms of onset of use of contraceptive methods (72.71±61.35 vs. 10.02±36.71 days post-partum; P=0.001).   Discussion & Conclusions: Appropriate education and consultation programs for young and inexperienced women can prevent pregnancies during breastfeeding. 3857 Bacteriology Phylogenetic Characterization oF Endophytic Bacterium Pseudomonas Protegens with Antagonistic Ability Isolated From privet Etminani Faegheh Etminani Adibeh Young Researchers and Elite Club Islamic Azad University, Sanandaj Branch, Sanandaj,Iran Young Researchers and Elite Club Islamic Azad University, Sanandaj Branch, Sanandaj,Iran 1 11 2018 26 4 74 84 05 10 2016 01 05 2017 Introduction: Endophytic bacteria are able to control plant pathogens by producing antagonistic compounds. This research was conducted to determine the antagonistic ability of bacterial endophytes in privet (Ligustrum vulgare L.).   Materials & Methods: In this study, endophytic bacteria were isolated from stem and leaf of privet (Ligustrum vulgare L.). After genomic DNA extraction, 16S rDNA gene was amplified using polymerase chain reaction (PCR) technique for precise bacterial identification. Then, the PCR product was sequenced by BLAST. Strains were examined for siderophore, hydrogen cyanide, and protease tests.   Findings: All the isolated bacteria were able to produce protease. Only one strain (BN3) was able to produce hydrogen cyanide. The siderophore test was positive for one strain (BN2). Based on the 16S rDNA sequence studies, the strain bacteria belonged to Pseudomonas protegens and indicated 100% similarity to type strain.   Discussion & Conclusions: The endophytic bacteria isolated in this study can be used to promote plant growth. Knowledge about endophytic bacteria-plant interaction can provide an effective strategy to develop sustainable agriculture in order to ensure yield improvement without affecting environment. ​ 3402 midwifery Estimation of Mean age of Menopause in Iran: A Systematic Review and Meta-Analysis Azadi Tayebeh Arghavani Hana Karezani Parvaneh Sayehmiri Kurosh Deputy of Research and Technology, Ilam University of Medical Sciences, Ilam, Iran Deputy of Research and Technology, Ilam University of Medical Sciences, Ilam, Iran Student Research Committee, Deputy of Research and Technology, Ilam University of Medical Sciences, Ilam, Iran Social Medicine Group, Ilam University of Medical Sciences, Ilam, Iran 1 11 2018 26 4 85 93 20 02 2016 24 06 2017 Introduction: Menopause is the most important event during the middle age period, which is characterized by cessation of menstruation for at least 12 months (not related to pregnancy, breast-feeding, or other hormonal disorders). Due to hormonal changes during this period, the risk of many diseases including osteoporosis and heart disease increases, therefore, studying this critical period of life seems essential.   Materials & Methods: Totally, 34 papers were collected and analyzed by searching Magiran, Irandoc, Google scholar, ISI, Cochrane, Embase, SID, and PubMed databases. To pool mean menopause age in the retrieved studies, we used mean, standard deviation, and sample size in each study. The weighted mean and normal distribution were used to synthesize results. Confidence intervals were computed using random effects model in meta-analysis.  The heterogeneity of the studies has been checked by I² statistics and the data were analyzed by applying R and STATA (Ver11/2) software.   Findings:  The total number of women in the studies was 27,250 with the mean age of 51.8 ± 4.79 years. The average age of menopause was 48.26 years (95% CI 46.85 to 49.67). Ten articles with the total sample size of 13,260 showed that the median of pregnancy age was 49.7 ± 1.52 years.   Discussion & Conclusions: According to the results of the recent studies and the low mean age of menopause in Iran compared to the developed countries, to reduce the complications of this important period of life, extended health programs are recommended to be planned and implemented to improve the quality of life in women experiencing menopause. 4283 genetic mlvkly Investigation of Methylation in E-cadherin Gene Promoter Regions and Interleukin-17 Gene Polymorphism in Breast Cancer Patients Naeimi Sirous Dept of Genetics, Faculty of Basic Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran 1 11 2018 26 4 94 103 29 04 2017 27 05 2017 Introduction: The E-cadherin gene is associated with poor prognosis and metastasis in patients with breast cancer, and methylation of its promoter is correlated with decreased gene expression. In this study, we aimed to evaluate the potential role of E-cadherin promoter methylation in breast cancer.   Materials & Methods: In this case-control study, 40 breast cancer patients and 40 healthy women were examined. DNA was extracted, and for gene promoter methylation and IL-17 gene polymorphism, MSPCR and PCR-RFLP methods were used, respectively. Data were compared in both groups by using Pearson’s correlation coefficient, Chi-square test, and Hardy-Weinberg equilibrium test.   Finding: We found a relationship between E-cadherin gene promoter methylation and breast cancer, such that the promoter of E-cadherin gene was significantly more methylated in patients compared to normal individuals (P<0.05).   Discussion & Conclusions: It seems that increased E-cadherin gene promoter methylation in patients is associated with the risk of breast cancer. 4263 animal physiology Effects of Dexamethasone on Regeneration Process after Sciatic Nerve Transect and Acellular Scaffold Graft in a Rat Animal Model Ghayour mohammad bagher Abdolmaleki Arash Behnamrasouli Morteza Moghimi Ali Mahdavishahri Naser Dept of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran . Dept of Engineering Sciences, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran Dept of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran Dept of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran Dept of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran 1 11 2018 26 4 104 115 18 04 2017 12 06 2017 Introduction: Peripheral nervous system has an innate regenerative ability, and in severe injuries leading to nerve destruction, repair does not occur spontaneously and medical intervention is required. In this regard, dexamethasone is one of useful drugs prescribed for neurological recovery after spinal cord injury. The objective of the present study was to assess the effect of dexamethasone on functional recovery and histological changes following sciatic nerve transect and regeneration with the acellular scaffolds in male Wistar rats.   Materials & Methods: Fifty male Wistar rats were randomly divided into five groups of 10. The groups included: negative control group(left sciatic nerve was transecte), sham-operated group) surgical procedure without the nerve transect ), and experimental groups 1 and 2 (sciatic nerve was transected and regenerated with acellular nerve scaffold) treated with 1 and 2 mg/kg of dexamethasone, respectively. Experimental group 3 (sciatic nerve was transected and regenerated with acellular nerve scaffold) was injected with vehicle. Then, 1 cm of left sciatic nerve was exposed and replaced with a piece of scaffold. Behavioral, electrophysiological, and morphological alterations were evaluated during the experiment.   Findings: with dexamethasone in comparison with the control group (P < 0.05). Analysis of gastrocnemius muscle mass in groups treated with dexamethasone in comparison with negative control group showed that muscle atrophy was reduced; also the dexamethasone-treated group presented significantly greater nerve fiber, axon diameter, and myelin sheath thickness (P < 0.05).   Discussion & Conclusions: The results showed that the administration of dexamethasone after sciatic nerve transects and grafting the acellular scaffold increased motor function in a rat animal model. 4085 Microbiology The Protective Effect of Conjugated Bacterial Melanin with Titanium Oxide against UV Rezaie Seyyed Ali Javadi Ali Kadkhodayi Zahra Zolfaghari Mohamad Reza Dept of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran Dept of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran Dept of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran Dept of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran 1 11 2018 26 4 116 125 15 01 2017 13 08 2017 Introduction: Some Azotobacter species can produce melanin. Melanin protects against UV. The purpose of this study was to isolate Azotobacter with the power of producing melanin and optimize its production and the effect of its combination with titanium oxide nanoparticles on the protection against ultraviolet light (UV).   Materials & Methods: Overall, 200 soil samples from different parts of Qom, Iran, were cultured on Ashby's mannitol agar medium using Bio-test method. The effect of optimization of conditions such as pH, temperature, glucose source, amino acid sources, and aeration on the amount of melanin production was assessed. Afterwards, melanin was extracted, conjugated with titanium nanosilicate compound, and then the effect of each of the compounds on Escherichia coli was examined under the influence of UV based on time.   Findings: Melanin-producing Azotobacter vinelandii species were isolated. The optimum pH was determined about 7.5. Glucose at different concentrations (1-6%) reduced the production of the product. Methionine amino acid had an inhibitory effect, while tyrosine amino acid increased the production rate. Tryptophan amino acid at 0.1-0.7% also increased the production rate, while cysteine and phenylalanine did not change the production rate. The combination of titanium oxide with melanin showed a significant protective effect against Escherichia coli bacterium under the influence of UV.   Discussion & Conclusions: Native isolates of Azotobacter isolated from agricultural soil with the power of producing melanin can be used as a source for its use in the industry. 4495 biochemistry Plasma Changes of some Biomarkers and Adenosine Deaminase Activity in Streptozotocin-Induced Diabetes Mellitus in Rats Following Administration of Different doses of Pure Extract of Cynodon dactylon Rhizome Digaleh Farid Azimzadeh Kaveh Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran Young Researcher and Elite Club, Urmia Branch, Islamic Azad University,Urmia, Iran 1 11 2018 26 4 126 135 05 08 2017 08 01 2018 Introduction: The aim of this study was to evaluate the changes of some biomarkers in streptozotocin-induced diabetic rats following the administration of pure extract of Cynodon dactylon.   Materials & Methods: In this study, after dividing 50 rats into five groups and induction of diabetes mellitus in four groups, pure extract of Cynodon dactylon was administered at 150, 300, and 450 mg/kg/day concentrations to three diabetic groups for three months. The positive control group (diabetic group without Cynodon dactylon administarion) along with negative control group (non-diabetic group) did not receive any extract. Afterwards, some biomarkers along with plasma adenosine deaminase (ADA) activity were assessed in all the groups.   Findings: The results showed a significant increase in all the inflammatory markers, including ADA Hcy, and cTnI (P≤0.01) and a significant reduction in marker of tissue damage, gelsolin (GLS), (P≤0.01) in the positive control group compared with the control group. We also found a significant decrease in these parameters (P≤0.01) and a significant increase in marker of tissue damage (GLS) following the administration of 450 mg/kg of the extract compared to the positive control and negative control groups.   Discussion & Conclusions: It can be concluded that the extract of Cynodon dactylon, especially at the concentration of 450 mg/kg of body weight, can significantly reduce cardiovascular and inflammatory biomarkers and elevate GLS concentration in diabetic rats. 4254 anatomy Curriculum Audit of the Iranian Anatomy PhD Program according to ORPHEOUS Standards: A European Standard Rezaietavirani Mostafa Sadeghi Yousef Kavarizadeh Forouzan Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Dept of Anatomy, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Depat of Medical Education, Faculty of Medical Science Education, Shahd Beheshti University of Medical Sciences, Tehran, Iran 1 11 2018 26 4 136 141 16 04 2017 16 04 2017 Introduction: Curriculum of Anatomy plays an important role in training experts in the Anatomical Science. Therefore, this study aimed to audit the Iranian curriculum for Anatomy PhD program according to European standards (ORPHEOUS).   Materials & Methods: This descriptive-comparative study initially investigated the content and structure of Iranian curriculum of Anatomy PhD program, and then compared it to ORPHEOUS standards. This European standard elaborates the curriculum in eight facets and identifies the level of alignment between Iranian and European curriculum with respect to each facet.   Findings: Our findings indicated that the Iranian curriculum for Anatomy PhD program was 100% in concord with the European standards in five facets including course programs, educational content, teaching methods, educational level, and role of graduates. However, two facets obtained 66% concordance to ORPHEOUS, namely educational structure and course-based teaching. Finally, the duration of Iranian program was only 50% similar to what ORHEOUS has recommended as the standard duration for PhD programs.   Discussion & Conclusions: This study revealed that three facets of the Iranian curriculum for Anatomy PhD program were not fully consistent with the European standards. These three facets were lack of provisional committee to monitor progress in students’ theses, lack of compatibility between the offered duration of study and the real time it takes to graduate, and lastly, lack of the program’s ability to attend in the international arena. Therefore, it is recommended to establish a provisional committee for monitoring progress in students’ theses and to revise the curriculum accordingly. 4169 General Increasing the Speed and Precision of Prediction of the Results of Angiography by Using Combination of Adaptive Neuro-Fuzzy Inference System and Particle Swarm Optimization Algorithm based on Data from Kowsar Hospital of Shiraz Ayat Saeed Dept of Computer Engineering and Information Technology, Faculty of Computer Engineering, Payame Noor University, Tehran, Iran 1 11 2018 26 4 142 154 28 02 2017 10 06 2017 Introduction: With regards to the importance of early prognosis of coronary artery diseases, in recent years the use of the latest artificial intelligence and data mining findings is considered to assist physicians. The purpose of this study was to increase the precision and prediction speed for the results of angiography by using a combination of fuzzy inference systems and particle swarm optimization algorithm.   Materials & Methods: A new system consisting of a combination of fuzzy inferences and particle swarm optimization algorithm was proposed and simulated by MATLAB software R2015a (8.5.0.197613). The samples consisted of 152 patients who were randomly selected from those undergone coronary artery angiographies in Kowsar Hospital of Shiraz, Iran, in August 2013. The data were then analyzed by Excel 2010 and the essential parameters of the proposed system were extracted.   Findings: The data were then randomly divided into 20 groups for training and testing. These groups were selected randomly in a manner that 85% of the data were used for training and 15% for testing, and each group was simulated individually. The results of the simulation after 20 rounds of simulation with different training and testing data in system performance indicators displayed that the average of sensitivity, specificity, precision, and accuracy was 0.8422, 0.9192, 0.8554, and 0.8888, respectively, and it was equal to 1 in the most optimal situations.   Discussion & Conclusions: High performance indicators prove that the proposed system has a satisfactory performance in predicting the results of angiography and classifying them into two classes of normal and patient. In fact, in this study, prediction speed and precision were improved by using the proposed system, which was based on neuro-fuzzy inference system in combination with particle swarm optimization meta-heuristic algorithm.   4646 gardiologist The Effect Rehabilitation Exercise on the Upper Crossed Syndrome in Patients with Coronary Artery Bypass Surgery Babakhani Farideh Rahmani Mahdi Barati Amir Hossein Dept of Sports Injury and Corrective Exercises, Faculty of Physical Education and Sport Sciences, Allameh Tabataba'i University, Tehran, Iran Dept of Sports Injury and Corrective Exercises, Faculty of Physical Education and Sport Sciences, Allameh Tabataba'i University, Tehran, Iran Dept of Sport Sciences, Faculty of Physical Education and Sport Sciences, Shahid Rajaee Teacher Training University, Tehran, Iran 1 11 2018 26 4 155 165 12 11 2017 18 03 2018 Introduction: Due to the importance of correct posture and the association of inappropriate posture with pain in the chest and scapula, the aim of this was to investigate the impact of rehabilitation exercise on the upper crossed syndrome in coronary artery bypass surgery patients.   Materials & Methods: The statistical population consisted of all the 44 to 70-year-old men with coronary artery bypass surgery and upper crossed syndrome referred to Valiasr Hospital of Qom. The participants included 30 subjects (experimental group 10, control group 10 and test group10) who were randomly selected to participate in the study. In the test group a day before surgery and 8 weeks after surgery and in the control and experimental groups, 8 weeks after surgery and 8 weeks after the first test, the associated tests were run. To analyze the data, t-test Wilcoxon, and analysis of covariance were used. Findings: According to the results of the experimental group, the exercises had a significant positive (p<0.05) effect on kyphosis and forward head postures and had a positive, but not significant, effect on rounded shoulders. In the test group, the trainings had a significant positive effect on kyphosis, forward head, and rounded shoulder on the left side (p<0.05), while it had a positive, but non-significant, effect on rounded shoulder on the right side.   Discussion & Conclusions: In the present study, combination trainings using isometric, tread band, and stretching movements were effective in correcting forward head, kyphosis, and rounded shoulder postures in patients undergoing coronary artery bypass surgery.   4584 Physical Education Effect of Two-Week Swimming Training on the Expression of Irisin Protein in Lumbar Spine of Rats with Neuropathic Pain Farzad Babak Shamsaei Nabi Rajabi Hamid Gharakhanlou Reza Jameie Seyed Behnamedin Neuroscience Research Center, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran. Dept of Physical Education and Sports Sciences, Faculty of Literature and Humanities, Ilam University, Ilam, Iran Deptt of Physical Education and Sports Sciences, Faculty of Physical education, Kharazmi University, Tehran, Iran Deptt of Physical Education and Sports Sciences, Faculty of Literature and Humanities, Tarbiat Modares University, Tehran, Iran Dept of Medical Basic Sciences, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran 1 11 2018 26 4 166 174 17 10 2017 25 11 2017 Introduction: Irisin is a myokine that is suggested to be a link between skeletal muscles and other tissues. Simultaneous presence of irisin and glutamate decarboxylase enzyme in purkinje cells of cerebellum led us to examine the potential role of irisin. As glutamate decarboxylase enzyme is responsible for converting glutamate excitatory neurotransmitter to GABA inhibitory neurotransmitters.   Materials & Methods: Forty male Wistar rats (weight: 180±20 g) were assigned to five groups as follows: 1) CCI neuropathic pain with swimming training (CCIST); 2) CCI neuropathic pain without swimming training (CCI); 3) No CCI neuropathic pain with swimming training (ST); No CCI neuropathic pain without swimming training (Control); 5) CCI sham surgery (Sham). CCI and CCIST groups underwent peripheral nerve injury by four loose ligatures on the sciatic nerve. The swimming training program included two weeks of swimming (5 sessions per week, 30-60 min per session). The protein expression of irisin in L4-L6 regions was evaluated by western blotting technique.   Findings: Irisin expression in the CCIST2 (P=0.039) and ST2 (P=0.016) groups was lower than in the CCI group. However, there was no significant difference between two training groups.   Discussion & Conclusions: If we assume a similar role for irisin in the nervous system as in adipose tissue, the lower level of irisin in the two training groups can be justified as it is less needed to reduce ATP production as an excitatory neurotransmitter in the spine.a 4977 biology The Inhibitory Effects of Silver Nanoparticles on Bap Gene Expression in Antibiotic-Resistant Acientobacter bumanni Isolates using Real-Time PCR Piri Gharaghie Tohid Sadat Shandiz Seyed Ataollah Dept of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran Dept of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran 1 11 2018 26 4 175 185 23 05 2018 12 09 2018 Introduction: Acinetobacter bomanni is one of the most common opportunistic pathogens in hospitals that are resistant to many antibiotics due to biofilm production. Nowadays, silver nanoparticles(AgNPs) have received wide attention in medicine due to their physical and chemical properties. This study aimed to evaluate the anti-biofilm activity of silver nanoparticles on antibiotic-resistant A. bumanni strains.   Materials & Methods: In this experimental study, A. bumanni was isolates from 100 clinical samples. After identification of A. bummani strains and determination of antibiotic resistant profiles, biofilm-producing isolates were determined using Congo red agar at 37°C after 24 hours. The minimum inhibitory concentration (MIC) of the strains against AgNPs was determined. After 24 hours of exposure of the strains to sub-MIC concentration of AgNPs, RNA extraction and cDNA synthesis were performed. Finally, Bap gene expression was measured using real time PCR method. Finding: Out of 100 clinical isolates, 12 belonged to A. bummani, and all the strains were resistant to antibiotics, except for colistin. Real-time PCR results show that all the strains had a significant down-regulation in Bap gene expression compared to the control gene (P<0.05). Discussion & Conclusions: According to the anti-biofilm effects of AgNPs, it seems that AgNPs can be used as drug candidates in pharmaceutical industries. 4947 animal physiology The Effect of Smyrnium cordifolium Boiss. Hydroalcoholic Extract on Addiction Withdrawal Syndrome in Mice in Comparison with Clonidine Nazari Pari Yaghmai Parichereh Rangin Alireza Abbasi Naser Dept of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Dept of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Dept of Biology, Ilam Branch, Islamic Azad University, Ilam, Iran Dept of Pharmacology, Ilam University of Medical Sciences, Ilam, Iran 1 11 2018 26 4 186 196 04 05 2018 28 08 2018 Introduction: Smyrnium cordifolium is used in traditional medicine to treat anxiety, pain, insomnia and complications of drug addiction syndrome, which is one of the concerns of every community. The use of opioid drugs repeatedly causes physical dependence and tolerance. Dependence can be assessed by the symptoms of sudden withdrawal of the drug by administering a drug antagonist or both. The purpose of this study was to investigate the inhibitory effects of hydroalcoholic extract of this plant on the disorders caused by the addiction withdrawal syndrome in comparison with clonidine.   Materials & Methods: In this experimental study, 48 mice (25-30 gr) were used and divided into 6 groups of 8 and were addicted during seven days. Saline group: this group was nonmorphine-dependent and received normal saline with the equivalent dosage. Groups 2, 3 and 4 treated with S. cordifolium hydroalcoholic extract (SCE): these groups received morphine and SCE (100,200,300 mg/kg) as gavage. Group 5 (clonidine): This group of mice received morphine and clonidine (0.2 mg/kg). Group 6 (control): this group of mice received just morphine. In all the groups, signs of withdrawal syndrome were recorded on the seventh day 30 minute after naloxone injection. The results were analyzed by one-way ANOVA and Tukey’s test at a significant level of P <0.05. Findings: The results of this study showed that the effect of extract on the number of jumping in the SCE100 compared to the clonidine group decreased significantly (P<0.05) and SCE200 decreased significantly (P<0.01). In the SCE300 group, there was a significant decrease in this regard (P<0.001) compared to the clonidine group. Also, the effect of SCE on the maen number of rearing was ineffective in the SCE100 group, and SCE200 decreased significantly (P<0.05). In the SCE300 group, there was a significant decrease in this respect (P<0.01) compared to the clonidine group. The effect of extract on the number of teeth chatering in the SCE100 group decreased significantly compared to the clonidine group (P<0.05) and SCE200 decreased significantly (P<0.01). In the SCE300 group, there was a significant decrease in this level (P<0.001) compared to the clonidine group.   Discussion & Conclusions: Regarding the results of this study, SCE was capable of reducing the signs of opiate withdrawal in morphine-dependent mice. It is likely to modify the symptoms of the syndrome by activating opioid, gabanergic and serotonergic pathways. However, further studies are needed to determine the exact mechanism of the effect of SCE. 4279 biochemistry Zizyphus jujuba Seed Essential Oil Inhibits Lipopolysaccharide-Stimulated THP-1 Cells Invasion and Migration through Suppression of Matrix Metalloproteinase-9 Expression Ghodousi Dehnavi Elham Maghsoudi Hossain Haji Hosseini Reza Dept of Biotechnology, Faculty of Sciences, Payame Noor University, Tehran, Iran Dept of Biology, Faculty of Sciences, Payame Noor University, Tehran, Iran Dept of Biotechnology, Faculty of Sciences, Payame Noor University, Tehran, Iran 1 11 2018 26 4 197 209 27 04 2017 19 08 2017 Introduction: Migration and invasion features, which were associated with inflammatory response, play a vital role in the pathogenesis of osteoarthritis. Metalloproteinases (MMPs) as one of the most important catabolic bio-markers play a critical role in THP-1 invasion and migration induced by lipopolysaccharides (LPS). The aim of this study was to investigate the anti-invasive and anti-migratory properties of Zizyphus jujuba seed essential oil by inhibiting metalloproteinase 1, 2, 3, and 9, which are expressed during the development of osteoarthritis.   Materials & Methods: The seeds of Z. jujuba were collected from Isfahan. The seeds were separated and the essence was extracted in the Center of Genetic Resources of Iran. The effect of Zizyphus jujuba seed essential oil on cell viability, migration, and invasion of THP-1 cells was analyzed using MTT assay and transwell chambers assay, respectively. The expression of MMP-1, 2, 3, and 9 genes was evaluated by Real-time PCR, protein expression with Western blotting and ELISA, and the activity of MMP-2 and MMP-9 was investigated by gelatin-zymography.   Findings: Zizyphus jujuba seed essential oil (50 μg/ml) could remarkably suppress the migratory and invasive capacity of THP-1cells stimulated with LPS compared with the control group (P<0.05). Results showed that Zizyphus jujuba seed essential oil significantly suppressed LPS-stimulated THP-1 migration and invasion by inhibiting MMP-9 expression and activity (P<0.05).   Discussion & Conclusions: Zizyphus jujuba seed essential oil could inhibit THP-1 migration and invasion induced by LPS by suppressing MMP-9 expression, providing a theoretical foundation for the clinical treatment of osteoarthritis with Zizyphus jujuba seed essential oil.