96 1563-4728 Ilam University of Medical Sciences 7161 Natural Resources Antimicrobial Effects of Aqueous-Alcoholic Extract of Artemisia Turanica against Methicillin-Resistant Staphylococcus aureus (MRSA): An in vitro Study Bagheri yazdi Hasan b Norouzi taheri Hamed c b Farhangian University ,Iran c Farhangian University, Mashhad, Iran. 1 6 2022 30 2 1 7 02 07 2021 09 01 2022 Introduction: Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are on the rise worldwide. Over the last few decades, new compounds of natural origins have regained interest as an alternative to antibiotics in the treatment of MRSA infections. This study aimed to investigate the antimicrobial effects of Artemisia Turanica extract, a native plant of Iran. Material & Methods: The plant was subjected to aqueous-alcoholic extraction, and its antibacterial effects were then evaluated using the disk diffusion method. All tests were performed in triplicate, and the mean diameter of the growth inhibition zone was recorded. Finally, the minimum inhibitory concentrations and minimum bactericidal concentrations (MIC and MBC) against MRSA were determined using the micro broth dilution method. (Ethic code: IR.MUMS.REC.1401.015) Findings: The recorded antimicrobial activity against MRSA of the plant extract (29±1.5) was higher than that of the vancomycin antibiotic (14±1.4). The effect of MIC on MRSA was recorded at 98 μg/mL, and the MBC was estimated at 157 μg/mL. The plant extract also showed antifungal activity against the tested pathogenic fungal strains. Discussion & Conclusion: Artemisia Turanica extract has a strong antibacterial activity against MRSA. Accordingly, the purification and identification of active compounds of this extract on an industrial scale pave the way for introducing new antimicrobial compounds.  
5682 medical education Explaining the Effective Dimensions and Components for Community-Based Medical Education from the Perspective of Family Physicians of Ilam University of Medical Sciences: A Qualitative Study Mirmoghtadaaie Zohreh sadat d Ahmady Soliman e Abdoullahi Hojjatolah f d Department of e-Learning, Virtual School of Medical Education and Management, Shahid Beheshti University of Medical e Department of Medical Education, Virtual School of Medical Education and Management, Shahid Beheshti University of Medical Sciences(SBMU), Tehran, Iran f Network Management and Health Promotion, Health Department, Ilam University of Medical Sciences, Ilam, Iran 1 6 2022 30 2 8 17 17 05 2019 01 02 2022 Introduction: Structural changes following the socialization of medical education in the last decade have not only been effective in transforming the medical and health care professions but also has an impact on issues related to the welfare and health of society. Despite the basic steps that have been taken in presenting such programs during various medical education courses around the world, little research has been conducted on the development and implementation of community-based medical programs in Iran. The present study aimed to explain the dimensions and components affecting the implementation of community-based medical education from the perspective of family physicians of Ilam University of Medical Sciences, Ilam, Iran. Material & Methods: This study was conducted based on the qualitative approach and content analysis method. The data were then collected using semi-structural interviews and an interview guide. The study population consisted of family physicians working at health centers affiliated to Ilam University of Medical Sciences, Ilam, Iran, in 2019. According to the data saturation, 30 cases were selected using the purposive sampling method. Data were analyzed through qualitative content analysis using the Burningham method and MAXQAD10 software. The validity of the research findings was ensured by members' review methods, researcher immersion, and the multidimensionality of the data with Goba and Lincoln criteria. (Ethic code: IR.SBMU.SME.REC.1397.035) Findings: According to the analytical findings of the interviews, 121 initial codes, 41 subcategories, 13 categories, and 5 main themes affecting the implementation of community-based medical education were obtained. The five main themes included "conceptual analysis", "professional credibility", "professional challenges", "realization", and "facing real conflicts". Discussion & Conclusion: The results showed that what is taught under the name of medical education in practice could not meet the real needs of society and hinders people from reaching an acceptable level of social health. Therefore, considering the dimensions arising from the views of family physicians in future planning can be implemented to compensate for the existing shortcomings. 7147 biotechnolohgy Editing of the MALAT1 Gene in MDA-MB-361 Breast Cancer Cell Line using the Novel CRISPR Method Ahmadi-Baloutaki Soraya g Doosti Abbas h Jaafarinia mojtaba i Goudarzi Hamedreza j g Dept of Genetic, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran h Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran i Dept of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran j Dept of Genetic, Faculty of Basic Sciences, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran 1 6 2022 30 2 18 31 21 06 2021 18 12 2021 Introduction: Long non-coding RNAs play an important role in regulating gene expression, RNA processing, histone modification, and rearrangement of chromatin genes. These molecules can also be involved in many biological processes, such as organogenesis, cell differentiation, development, genome imprinting, quantitative compensation, and tumorigenesis. High expression of MALAT1 (a type of lncRNA) in many cancers, including breast cancer, indicates that a disorder of MALAT1 regulation is an important factor in the development of many types of cancer. Breast cancer is the most common cancer among women worldwide, and the invasion, as well as metastasis of this disease, are considered among the main causes of death. The present study aimed to knock out the MALAT1 gene in the MDA-MB-361 breast cancer cell line and evaluate its function and effects on the expression of genes associated with apoptosis. Material & Methods: In this study, two types of sgRNA were designed by CHOPCHOP software for exon 1 of the MALAT1 gene. These sgRNAs were cloned separately into two CRISPR vectors to generate the recombinant vectors PX459-sgRNA1 and PX459-sgRNA2. Co-transfection of these two recombinant vectors into the MDA-MB-361 cancer cell line was performed using lipofectamine 2000. MALAT1 gene editing was investigated in the cells receiving recombinant vectors. The expression of genes related to apoptosis was analyzed by Real-Time PCR. Cell proliferation and apoptosis were assessed by MTT and flow cytometry methods, respectively. (Ethic code: IR.IAU.M.REC.1399.010) Findings: The MALAT1 gene was edited by the CRISPR method in MDA-MB-361 cells. The rate of cell proliferation in the cells of the treatment group, compared to the control groups, showed a significant decrease (P<0.05). Apoptosis levels were significantly increased in cancer cells the MALAT1 gene of which had been deleted. Moreover, the expression of BCL2 and survivin anti-apoptotic genes in treated (edited) cells was significantly reduced, compared to control cells (P<0.05). Increased expression of proapoptotic genes P53, BAK, BAX, and FAS was also observed in the edited cells (P<0.05). Discussion & Conclusion: The results of this study confirm that the deletion of the MALAT1 gene has a significant effect on increasing apoptosis and reducing cell proliferation. A reduction in the expression of the MALAT1 gene can prevent the growth and proliferation of breast cancer cell lines. Therefore, it seems that the control of MALAT1 oncogene expression is useful and effective for controlling tumors.   6983 General Study of Human Albumin Protein Interaction with Fluorouracil Anticancer Drug Using Molecular Docking Method Motaharinia Mohammad k Sadeghpour Mahdieh l Shalbafan Monir m k Dept of Chemistry, Takestan Branch, Islamic Azad University, Takestan, Iran l Dept of Chemistry, Takestan Branch, Islamic Azad University, Takestan, Iran m Dept of chemistry, Imam Khomeini International University, Qazvin, Iran 1 6 2022 30 2 32 40 30 01 2021 29 11 2021 Introduction: Drugs are mainly delivered to the target tissues by plasma proteins, such as human serum albumin, in the human body. Practical information about the thermodynamic parameters of drugs and their stability can be obtained using simulation methods, such as molecular docking. Material & Methods: This study, investigated the molecular docking of human serum albumin with fluorouracil anticancer drug. Moreover, partial charges on serum albumin protein atoms and fluorouracil atoms were calculated in this study. The best configuration was also searched using the Lamarckian genetic algorithm. The dimensions of the grid maps were selected to be about 40 * 40 * 40 angstroms with a distance of 0.375 angstroms. The number of genetic algorithms and the number of studies were adjusted to about 100 and 2.5 million, respectively. In the end, the best performed interaction configurations with the least amount of free energy were selected. Ligplot and VMD graphic software were used to view the performed docking. Findings: In the best model, fluorouracil is able to bind to the human serum albumin protein HSA four hydrogen bonds via nitrogen and oxygen atoms with two amino acids tyrosine, one amino acid histidine and one amino acid arginine. The estimation of the free bond energies (kcal/mol) for the best model was -5.1. Negative Gibbs free energy values (ΔG °) indicated a spontaneous process, and a constant binding value (Ka ≈ 109 L • mol-1) demonstrated the optimal biological distribution of the drug in the blood plasma. Discussion & Conclusion: The docking study of the proposed models shows that fluorouracil has an aliphatic ring and hydrophobic fractions and therefore it has a high ability to form hydrophobic interactions with major amino acids at the active site of serum albumin protein.   7174 Bacteriology Synergistic Effect of Silver Nanoparticles and Streptomycin Antibiotic on the MexX Gene Expression of Pump Efflux System in Drug-Resistant Pseudomonas aeruginosa Strains Mohammad Ali n Molavi Farahnaz o Dolatabadi Samaneh p n Dept of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran, Mashhad, Iran o Dept of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran p Dept of Biology, Neyshbour Branch, Islamic Azad University, Neyshbour, Iran 1 6 2022 30 2 41 50 11 07 2021 01 12 2021 Introduction: Pseudomonas aeruginosa is one of the most important infectious agents in humans, which is difficult to control in hospitals due to its resistance to various antibiotics. Efflux pump systems play an important role in the drug resistance of this bacterium to a variety of antibiotics. This study aimed to determine the antimicrobial synergistic effect of silver nanoparticles and the antibiotic streptomycin on the MexX gene expression. Material & Methods: In this cross-sectional descriptive study, 49 samples were collected from 11 medical diagnostic laboratories in Mashhad from 1398 to 1399. After the treatment of multidrug-resistant bacteria with inhibitors, the microdilution method and Real Time-PCR technique were used to determine the effective dilution of silver nanoparticles and probiotics on the expression of the MexX gene of the bacterium. (Ethic code: IR.IAU.MSHD.REC.1400.018) Findings: All 49 collected samples of Pseudomonas aeruginosa were identified. All strains had the MexX gene, and all were resistant to more than two antibiotics. The minimum inhibitory concentration (MIC) results and expression of the MexX gene showed that the MIC in the agar dilution method for silver nanoparticles was up to 500 μg/ml. Evaluation of the synergistic effect of silver nanoparticles with streptomycin antibiotic showed that plates containing streptomycin antibiotic disk with silver nanoparticles with the dilution of 250 μg/ml caused a growth inhibition zone according to the CLSI standard. Silver nanoparticles with streptomycin synergy had a greater effect in inhibiting bacterial growth, and this effect was greater than that of silver nanoparticles (P>0.05). Discussion & Conclusion: Silver and streptomycin have inhibitory and antibacterial activity to reduce the function of the MexXY-OprM efflux pump in Pseudomonas aeruginosa (P>0.05).   7314 psychology The Effect of Acceptance and Commitment Therapy on Muscle Pain in Patients with Rheumatoid Arthritis Malek-Mohammadi-Memar Soheyla Zoghi Paydar Mohammad Reza Department of Clinical Psychology, Faculty of Medical Science, Hamedan branch, Islamic Azad University, Hamedan, Iran Department of Psychology, Faculty of Economic and Social Science, Bu-Ali Sina University, Hamedan, Iran 1 6 2022 30 2 51 62 13 10 2021 07 02 2022 Introduction: Rheumatoid arthritis is an autoimmune and inflammatory disease that can cause chronic pain. The aim of this study was to investigate the effect of acceptance and commitment therapy on muscle pain in patients with rheumatoid arthritis. Material & Methods: This research was a Quasi-experimental design with experimental and control groups. The study population consisted of 245 patients with rheumatoid arthritis under treatment in the physiotherapy ward of Shaheed Beheshti hospital during 2019 in Hamadan. A sample of 30 subject were selected and randomly assigned in two groups of 15 (experimental and control). The pre-test was then performed on both groups. The intervention was performed on the experimental group in 8 sessions (one hour) and the control group did not receive any intervention. After the intervention, post-test was performed in two groups. The research tools included Von Korff et al (1992) chronic pain rate questionnaire. Analysis of covariance used to analyze the data. Findings: The results of analysis of covariance show that there is a significant difference between the mean score of the subscale of pain intensity in the experimental and control groups (P <0.05, F = 5.84). The effect of acceptance and commitment treatment on the severity of muscle pain in patients was 0.18. The results of analysis of covariance also show that there is a significant difference between the mean score of the subscale of disability caused by pain in patients in the experimental and control groups (P <0.05, F = 6.52). The effect of acceptance and commitment treatment on disability due to muscle pain in patients was 0.20. Discussion & Conclusion: The results indicate that acceptance and commitment therapy can be used for reduce the severity of pain and disability caused by pain in patients with muscle pain.   7269 clinical psychology Role of Experiential Avoidance and Alexithymia in the Prediction of Psychosomatic Disorder among the Employees at National Iranian South Oil Company Molaei pardh Ali Karami Jahangir Moradi Asieh Department of Psychology, Faculty of Social Sciences, Razi University, Kermanshah, Iran Department of Psychology, Faculty of Social Sciences, Razi University, Kermanshah, Iran Department of Psychology, Faculty of Social Sciences, Razi University, Kermanshah, Iran 1 6 2022 30 2 63 70 09 09 2021 11 12 2021 Introduction: Psychosomatic disorder is one of the most common mental disorders in which meaningful psychological events play an effective role. This study aimed to investigate the role of experiential avoidance and alexithymia in the prediction of psychosomatics disorder among the employees at the national Iranian south oil company. Material & Methods: The statistical population of this descriptive correlational study included all employees at the national Iranian south oil company from 2020 to 2021. The sample size of the present study was obtained at 371 people based on Cochran's formula. Due to the possibility of incomplete questionnaires, 418 employees were selected by the available sampling method. They were then requested to complete the Symptom Checklist-90 (SCL-90), experiential avoidance (AAQ), and alexithymia (FTAS-20). (Ethic code: 140090) Findings: The results of the Pearson correlation coefficient showed a correlation coefficient of 0.39 between experiential avoidance and psychosomatic disorder which was significant at 0.001. Moreover, there was a correlation coefficient of 0.15 between alexithymia and psychosomatic disorder, which was significant at 0.003. In addition, the results of linear regression analysis showed that experiential avoidance could predict psychosomatic disorders. Discussion & Conclusion: Therefore, according to the findings, in order to reduce psychosomatic disorders, it is necessary to conduct interventions on psychological flexibility and emotional expression. Finally, it is suggested that other empirical and quasi-experimental studies be conducted to confirm these results. 7236 Physical Education Effects of Eight Weeks of High-Intensity Interval Training on the Expression of the Nkx2.5 and Tbx5 Genes in the Heart Tissue of Type 2 Male Diabetic Rats Kousha Mahdieh Abednatanzi Hossein Gholami Mandana Ghazalian Farshad Dept of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran Dept of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran Dept of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran Dept of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran 1 6 2022 30 2 71 81 18 08 2021 12 12 2021 Introduction: Exercise and antioxidants consumption are known as protective effect against the risk of diabetes. Therefore, the current study aimed to investigate the effects of eight weeks of high-intensity interval training (HIIT) and thyme honey on the expression of Nkx2.5 and Tbx5 genes in the heart tissue of male type 2 diabetic rats. Material & Methods: This experimental study was performed on 36 male diabetic rats which were randomly divided into four groups of control (n=8), HIIT (n=10), thyme honey (n=8), and HIIT+thyme honey (n=10). The HIIT training intervention was conducted in eight weeks (five sessions per week) and included 2 to 8 intervals with 2 min running at 80%-90% VO2max and 1 min at 50%-56% VO2max. Additionally, the supplement groups consumed 3 g/kg of thyme honey, 5 days/week. Both Nkx2.5 and Tbx5 gene expression in heart tissue were measured using real-time RT-PCR. To evaluate the differences among the groups, ANOVA and LSD post hoc tests were used at the significance level of P≤0.05. (Ethic code: IR.SSRC.REC.1399.080) Findings: The results revealed that the expression of the Nkx2.5 gene was significantly increased only in the HIIT group, compared to the control group (P=0.03); however, it was not observed in other intervention groups (P>0.05). The expression of the Tbx5 gene was significantly increased in both HIIT (P=0.02) and HIIT+thyme honey (P=0.02) groups, compared to the control group. Discussion & Conclusion: The HIIT is associated with increased expression of the Nkx2.5 and Tbx5 genes in the heart tissue of diabetic rats; however, it can not be said exactly that thyme honey has similar effects in diabetic rats.   7265 psychology Comparison of Effectiveness of Acceptance and Commitment Therapy (ACT) and Compassion-Focused Therapy (CFT) on Internalized Shame in Patients with Vitiligo Solimanpour Mahdi Pirkhaefi Alireza Zahrakar Kianosh Department of Human Science, Science and Research Branch, Islamic Azad University, Tehran, Iran Department of Clinical Psychology, Faculty of Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran Department of Counseling, Faculty of Psychology and Educational Science, Kharazmi University, Tehran, Iran 1 6 2022 30 2 82 96 07 09 2021 12 02 2022  Introduction: Skin diseases, not only may have physical consequences but also costly psychological consequences in one’s life. The present study was conducted to compare the effect of group counseling with the approaches of Compassion-Focused Therapy (CFT) and Acceptance and Commitment Therapy (ACT) on the internalized shame of patients with vitiligo. Material & Methods: This was a quasi-experimental study with pre-test and post-test design and follow-up and control groups. The study population consisted of all women with vitiligo who were referred to Razi Hospital, in Tehran, in 1399. A total of 45 persons who met the inclusion criteria were purposefully selected and randomly divided into 3 groups of 15 people each (two experimental groups and one control group). The study tool included Cook's Internalized Shame Scale (1993). Descriptive statistics and repeated measures analysis of variance was used to test the research hypotheses. The data were analyzed using SPSS software (version 20). (Ethic code: R.IAU.SRB.REC.1399.125) Findings: Both CFT and ACT therapeutic approaches were effective in reducing internalized shame in patients with vitiligo, compared to the control group. A comparison of the two approaches showed no significant differences between the two experimental groups of CFT and ACT in terms of all components of shyness, self-esteem, and internalized shame. Discussion & Conclusion: Based on the obtained results, the above-mentioned treatments can be used to improve the psychological status of patients with vitiligo.   7189 biochemistry Investigation of the Relationship between the rs8192688 Polymorphism of the FABP-4 Gene and Cardiovascular Disease Susceptibility in Type 2 Diabetic Patients in Ilam Province, Iran Bakhtiyari Amin Bakhtiari Salar Peymani Maryam Haghani Karimeh Norozi Siros Dept of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran Dept of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran Dept of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran Dept of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran Dept of Cardiology, Ilam University of Medical Sciences, Ilam, Iran 1 6 2022 30 2 97 104 17 07 2021 18 07 2021 Introduction: Cardiovascular disease (CVD) is the most common cause of death in patients with type 2 diabetes (T2D). The association between diabetes and cardiovascular disease is complex and multifaceted. This study aimed to investigate the possible association between rs8192688 Polymorphism of the FABP-4 gene and the CVD susceptibility in T2DM patients in Ilam Province, Iran. Material & Methods: The statistical population consisted of 210 cases (T2D patients with CVD [n=70], T2D patients without CVD [n=70], and healthy controls [n=70]). The frequency of polymorphisms was investigated using the Tetra-Primer-ARMS-PCR method. (Ethic code: IR.IAU.SHK.1399.011) Findings: The results showed that TG, HDL-C, and LDL-C in the T2D group with CVD were significantly increased, compared to those in the T2D group (P<0.05). The frequency rates of the CT genotype in the control, T2D, and T2D with CVD groups were 26%, 24%, and 295 (P=0.16), respectively. Moreover, the frequency rates of the TT genotype were 6%, 4%, and 4% (P=0.12), and the frequency rates of the T allele were 19%, 16%, and 19% (P=0.09), respectively. There was no significant relationship between this polymorphism and the studied groups. Discussion & Conclusion: The results showed that TG, LDL-C, and HDL-C could have a role in the development of CVD in T2D patients; however, the rs8192688 polymorphism of the FABP4 gene was not involved in the development of CVD in T2D patients.