@article{ author = {abbasiasl, m and salehi, m and fatemehhosaini, a and zayeri, f and nasrollahi,}, title = {Clustering of Provinces based on health indices in Iran: 2005}, abstract ={Inharmonious growth and mutation in provinces of country in cultural, economic and social characteristic in recent years, requires carefully plan for justly development in different areas. These plans essentially have to be integrated with inherent features of each zone and then, these plans are well enough to improve the quality of promotion. This study has been done according to health indices in Iran in order to identifying different poles over the country. Methods Homogeneous clusters have been calculated by using fuzzy clustering method and R statistical software, based on health indices in year 1385. The data are collected in help of department of Health in the Ministry of Health and Medical Education. Results Firstly, the Provinces of country have been divided to two clusters based on a cluster validity index. Then, again in order to minimize the length of each cluster, clusters repartitioned to two new clusters based on the validity index. The resulted clusters are homogeneous in structure. Discussion Based on geographic pattern of clusters and analyzing the status of each cluster and considering the advantages and disadvantages of them, it is possible to propose good health and care services in each province with respect to the inherent properties of each province.}, Keywords = {Fuzzy clustering, Health Status Indexes, degree of membership, Silhouette}, volume = {20}, Number = {5}, pages = {1-9}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-922-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-922-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {fazeli, z and fazelibavandpour, fs and rezaeetavirani, m and mozafari, m and haidarimoghadam, r}, title = {Professional ethics and its role in the medicin}, abstract ={Today, the ethical responsibilities are defined in the wide rang of individual responsibilities to professional responsibilities. Professional ethics is one of the important challenges in men’s society. If the definition of Professional ethics would be clear, the professionals in any fields could simply make their own decisions about the right or wrong actions and about the ethical or non-ethical behaviors. In the medical community, ethics has get lots of attention because of its special position. Therefor medical ethic was discussed in the wide range of scientific issue. If the benefits of professional ethics are inclusive, its commitment will be to the principles of the higher grade and how these limitations are more logical , more people do them.}, Keywords = {ethic, professional ethics,medical ethics}, volume = {20}, Number = {5}, pages = {10-17}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-921-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-921-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {kohansal, sajede and kohpaei, alireza and gharliporgharghani, zabiholah and habibi, peymane and zeyaei, mansoor and gilasi, h and haidarimoghadam, r and ferasati, f}, title = {Ergonomic evaluation of musculoskeletal disorders among kitchen workers by QEC technique in the Tehran University of Medical Sciences}, abstract ={Abstract Introduction: Musculoskeletal disorders (MSDs) represent one of the leading causes of occupational injury and disability in the developed and developing countries. For this reason, many researchers have been conducted on MSDs and risk factor evaluation techniques. Current study was conducted among workers of a kitchen Iran university of medical sciences with the objective of determination of WMSDs prevalence. The results of this study can used in order to develop WMSDs preventive strategies in the work place and improve worker’s health status. Materials ;Methods: in this descriptive cross-sectional study, all university's kitchen members were studied. Totally, 21 workers participated. Body ness Questionnaire was used to study prevalence of WMSDs and QEC technique was applied to evaluate physical exposure to the risk factors. Picturing technique was used to collect required data from each worker and each workstation. All of the 21 workers were photographed. Findings: 72.4% of the workers suffered from some kind of symptoms during 12 months. The highest prevalence was reported in lower back (48.2%), neck (34.4%) and upper back (26%).Furthermore, based on the results, QEC was found to be an appropriate and reliable technique for evaluating level of exposure to WMSDs risk factors. Discussion ;Conclusion: This study shows that WMSDs occurred at a high rate in the studied population. Major ergonomic problems were found to awkward postures, manual material handling and back rotation and bending .It is necessary to do some implementation of interventional and corrective measures in order to reduce the risk of WMSDs.}, Keywords = {Keywords: Posture, Musculoskeletal disorders, QEC technique }, volume = {20}, Number = {5}, pages = {18-23}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-923-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-923-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {motamedian, sr and khosraviani, k and gholipour, f and gholamin, p and fili, f}, title = {Craniomaxillofacial bone tissue engineering: A review on growth factors}, abstract ={Currently autografts are gold standards for craniomaxillofacial bone regeneration. But autografts have some limitations. Bone tissue engineering has been introduced to overcome these limitations. It consist three components: stem cells, growth factor, and scaffold. Scaffold provides an environment for bone growth and simplifies cell adhesion, differentiation and proliferation. In this review, application of natural, synthetic and composite scaffolds in animal and human studies were described. Based on this review, autografts are still gold standards for bone regeneration. However, it seems that recent advances in technology of designing scaffolds would help designing more appropriate scaffolds for bone tissue engineering in near future.}, Keywords = {Tissue engineering, Bone regeneration, Scaffold, Stem cells, Craniomaxillofacial}, volume = {20}, Number = {5}, pages = {23-29}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-924-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-924-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {biazar, esmaeil and heydari, saeyd and Heidarbeigi, kh and shohani, b}, title = {Behavioral evaluation of regenerated rat sciatic nerve by a nanofibrous PHBV conduit filled with Schwann cell as artificial nerve graft}, abstract ={Abstract Introduction: Recent evidence suggests that the implantation of artificial conduits improves peripheral nerve regeneration. Materials & Methods: We have developed an electrospun biodegradable poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nerve conduit filled with allogeneic Schwann cells (SCs). The nanofibrous conduits were investigated by physical, mechanical and microscopic analyses. The nerve conduits with and without Schwann cells were implanted into a 30-mm in sciatic defect of rats. All groups were monitored and investigated their functional and locomotion activities by behavioral analyses: Toe out angle (TOA), Toe spreading analysis, and Nociceptive function (WRL), monthly through four months post surgery. Finding: Results of physical and mechanical and microscopic analyses showed suitable properties of designed conduits to serve as a nerve guide, also Schwann cells well attached on nanofibrous surfaces of designed conduits. Four months post-operatively, the results from behavioral analyses were demonstrated that, in the grafted groups especially conduits filled with Schwann cells, the rat sciatic nerve trunk had been reconstructed with functional recovery such walking, swimming and recovery of nociceptive function. Discussion & Conclusion: This study proves the feasibility of the artificial nerve conduit filled with Schwann cells for peripheral nerve regeneration by bridging a longer defect in an animal model.}, Keywords = {Key words: Nanofibrous PHBV conduit, Schwann cells, Peripheral nerve repair, Behavioral analysis, Sciatic nerve.}, volume = {20}, Number = {5}, pages = {30-36}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-925-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-925-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {gilasi, hamidreza and moradi, ali and gharlipor, zabiholah and moazamigodarzi, ali}, title = {Knowledge, Attitude and Practice of Pedestrians about Driving Rules in kashan}, abstract ={Introduction: Iran is one of the countries with the highest number of deaths from road accidents. As the incidence of these injuries (30 in ten thousand) is higher than the regional and international rates and about 13.5% of the deaths are caused by traffic accidents. Since pedestrians are one of the groups at risk, the purpose of this study was to determine the knowledge, attitude and practice of them regarding to the driving rules. Materials & Methods: The current cross-sectional study was conducted on a target population of pedestrians in the city of Kashan. The sample size was 398 subjects that were selected by convenient method from forty spots on the streets of kashan. The Knowledge, attitude and practice of pedestrians were determined and the relationships between demographic and behavioral variables were assessed by the statistical tests, chi-square, odds ratio and its confidence interval. The reliability of questions in each domain was assessed by the using of Cronbach's alpha index. Construct validity of questions in each area was calculated by using of the principal components analysis method. Findings: A total of 398 pedestrians were studied. 54%, 61.3% and 32.2% of those surveyed people had an appropriate knowledge, attitude and practice regarding the driving rules, respectively. This study showed that the knowledge and attitude of pedestrians about road rules had statistically a significant relationship, however, the attitude and knowledge of pedestrians in this area was not statistically significant. Discussion & Conclusion: This study showed that the overall practice of pedestrians regarding driving rules in the city of Kashan was significantly poor. Given the importance of human factors in traffic accidents, the best way to reduce the occurrence of such events is the implementing of educational and preventive programs.}, Keywords = {pedestrians, knowledge, attitude, behavior, road rules, Kashan}, volume = {20}, Number = {5}, pages = {37-42}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-927-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-927-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {mohamadkhah, f and aminshekravi, f and faghihzadeh, s and babaeehaidarabadi, a and kazembegi, f and maghsodi, r}, title = {Comparison of two methods of dental health education lectures and film screenings on knowledge, attitude and practice of students}, abstract ={Bacgrond and Aim: Oral health is an important component of lifestyle. And recovery requires proper training methods to improve knowledge, attitudes and practices in relation to its population. The aim of this study was to compare the effect of lecture and video screening in improving the knowledge, attitude and practice of dental hygiene students. Methods:The survey is a quasi-experimental intervention survey done in Chabahar in 2011-2012. By sampling a regular allocation process is divided into three equal groups (each group n = 100:control, lecture and film) were divided..Knowledge, attitude and Practice student were measures by self report questionnaires and pre-test and post-test . And relevant data was analysed by using of software SPSS VERZHON 11, statistical tests ANOVA, Mann-Whitney. Results :The Lecture And Film Group knowledge And attitude immediatelly and 3 months after the intervention, the practice immediately after the intervention increased But A more effective approach to video on improving the knowledge, attitude and practice of oral health revealed (P <0/001). Conclusion:This study showed that both the training lectures and video display can be an effective way to promote Oral health Education Knowledge , attitudes and practice of the people Although video method is more effective.}, Keywords = {lectures, video screenings, awareness, attitude, behavior, }, volume = {20}, Number = {5}, pages = {43-50}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-926-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-926-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {zamanianazodi, m and ardashirilajimi, ar and ahmadi, n and gilanchi, s and abbasi, n and hematian,}, title = {The Study of Antibiotic Properties of Scrophularia Striata Aqueous Extract on Staphylococcus aureus}, abstract ={Introduction: Bacteria have become resistant to some kinds of antibiotics. Therefore, more reliable sources are vital to be examined. Some types of herbals have been shown promising for therapeutic points, so they could be applied in the field of medicine. Scrophularia consist of different biological activities such as antimicrobial, antitumor and anti-inflammatory activities. Consequently, its effect on Staphylococcus aureus has been studied. Material & Methods: Antibiotic effects of both filtered and non-filtered extract of Scrophularia striata on Staphylococcus aureus as gram-positive bacterium were evaluated by spectrophotometer. Simultaneously tetracycline was used as a positive control at different concentrations. In addition, MTT assay was applied for cell survival determination. Findings: Our findings point out that concentration between 1 to 20 µg/ml of the extract has remarkable antibiotic activities. In addition to this, there is no obvious difference between filtered and non-filtered antibiotic activities of this extract. On the other hand, fibroblast cells show absolutely different response when treated with the same extract. Discussion & Conclusion: It can be concluded that, the Scrophularia striata aqueous extract has significant antibiotic activity and with less side effects than tetracycline. As a result, Scrophularia striata extract can be regard as a probable antibiotic agent in the future studies additionally, more investigation is needed for evaluating in vivo outcomes, and the effective materials of the extract component}, Keywords = {Scrophularia striata; Antimicrobial Effects Staphylococcus aureus; Human Fibroblast Cell line, Tetracycline }, volume = {20}, Number = {5}, pages = {51-58}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-928-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-928-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {nadri, hamed and nikpey, ahmad and nadri, farsad and rghalenavi, mehran and SAFARIVARYANI, ali and AVAZPOR, MOAYED and mirzaei, feyzolah}, title = {Measurement and design of general illumination in Qazvin Medical science University student residences}, abstract ={Abstract: Introduction: Adequate lighting in halls study and student dormitories plays an important role in focus, motivation and accuracy of student. Material and Method: The purpose of this study measured and design of general illumination in Qazvin University of Medical Sciences student dormitories, the same order of existing residences (dormitories 7) randomly selected 81 bedrooms & all study halls were also measured. To measure the general illumination intensity using the public network, first level of stations into the desired location and in the center of each station, 75 cm in height from ground level at night, And in the absence of all light bulbs were available measurements were performed. To measure the general illumination intensity EC Hagner luxmetr was used, Results were compared with standard by using Excel software. Findings: The results showed that 74/81percent of bedrooms with intensity less than the Minimum level of Iran (150 lux) and 100 percent of Study halls were illumination intensity lower than a Minimum level of Iran (300 lux). According to results No 7 dormitory bedrooms have a lowest intensity (131/2 lux) and No. 2 dormitory study hall have a lowest intensity (125/5 lux). Discussion & Conclusion: Poor illumination in the dormitories mainly were due to poor alignment and lack of light bulbs, that today by using lighting design software ,This deficit will be resolved. So continue by using DIALUX software, Number and arrangement of lamps for suitable lighting in study halls and bedrooms design and presented .}, Keywords = {Key words: illumination intensity, DIALUX software, study hall, measurement station}, volume = {20}, Number = {5}, pages = {59-66}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-929-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-929-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {motamedian, s and rohani, k and javand, f and shirkavand, s and khosraviani, k and gholipour, f and esmaeinezhad, a and rozegar, m}, title = {Laser Applications in Implantology}, abstract ={In modern implantolotology there are several indications for laser. Different laser systems can be applied in soft and hard tissues surgery. In this review, literature was searched for application of laser in second-stage surgery of implants, treatment of peri-implant bony defects, removal of peri-implant hyperplastic tissues, and the preparation of bone cavities for implant placement. This report describes the application of different laser systems in treatment of peri-implantitis. Our study evaluated in vitro, animal and clinical examinations. The exact selection of the appropriate laser system was dependent on the evaluation of recent literature, the effect of laser on implant surface and, the level of temperatures rise during laser application. Two major advantage of laser which are reduction in bacteriae and the cutting effects associated with the coagulation properties of the lasers are the main reasons for laser application in the treatment of peri-implant lesions and can improve the prognosis of ailing implants.}, Keywords = {Laser, Implant}, volume = {20}, Number = {5}, pages = {75-83}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-930-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-930-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {azizian, r and azizijalilian, f and askari, h and naser, a and karimi, s and sadeghifard, n and mosavinasab, sd and ahmadi,}, title = {Bacteriophage as a new candidate for the prevention and removal of biofilms}, abstract ={Background: Pseudomonas aeruginosa is a ubiquitous organism which has emerged as a major threat in the hospital environment. Overuse of antibiotics has also significantly increased the emergence of antimicrobial multiresistant bacteria. P. aeruginosa has an innate ability to adhere to surfaces and form virulent biofilms. Bacteriophage might represent one attractive solution to this problem. In this study, P.aeruginosaphage was utilized to Biofilm prevention and removal. Methods: After isolation of phages and serial dilution microplate method of biofilm formation was examined. Furthermore, the effects on biofilm bacteria isolated phages were determined on the Pseudomonas putida, Acinetobacter baumanii and E.coli. Result: P.aeruginosa biofilm had OD: 1.688 in 492n.m. Pure phage, 10-2 and 10-3 diluted phage decreased OD to 1.587, 1.341 and 1.461,respectively.Isolated phage dramatically decline OD of Biofilm of all strains. Discussion: Phages have various affinity to attach to hosts, thereby it is supposed to phages compete for their receptors.Therefore it is supposed phages have most efficiency in optimum concentration to remove biofilm or growth inhibition. Also, funding prove phages have effect on various bacteria and they can use to remove different strains.}, Keywords = {Key word: Bacteriophage; Pseudomonas aeruginosa; Biofilm}, volume = {20}, Number = {5}, pages = {84-93}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-931-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-931-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {aghaalikhani, m and taheritanjani, p and hossainpourazari, a and mosavi, m and rezapour, m and amini, y and hemati, f}, title = {Prevalence of osteoporosis among above 60 years old subjects referred to Ayatollah Taleghani hospital in Tehran and its relationship with age and sex}, abstract ={Introduction: Osteoporosis is a common bone disease that leads to disability and increased mortality due to bone fracture. This study was designed to evaluate the prevalence of osteoporosis and its association with gender and age in patients referred to elderly clinic in Taleghani hospital, Tehran. Method: In this descriptive-analytic study, from 580 subjects referred to the elderly clinic of Taleghani hospital, 131 subjects that have BMD file were selected and densitometry results were collected from densitometry records. Data were analyzed by SPSS software using Chi-Square, T-test and linear regression. P<0.05 was considered as significant. Results: The overall prevalence of osteoporosis and osteopenia was 19.9% and 36.2 percent. The overall prevalence of osteoporosis was 29.8% and 16.8% in lumbar and femoral region, respectively. Mean lumbar and femoral T-score were -1.692 ± 1.50 and -1.51 ± 1.17, respectively. Association between age and osteoporosis in lumbar region and femoral region was significant (P value <0.05). Association between gender and osteoporosis in lumbar region was significant but in femoral region was not significant. Linear regression between Lumbar and femoral T-score was +0.632 (P value<0.0001) Conclusion: Our finding revealed a higher prevalence rate of osteoporosis in patients referred to elderly clinic of Taleghani hospital and showed that age in femoral and lumbar region can predict lumbar and femoral density.}, Keywords = {Osteoporosis, Prevalence, Sex, Age.}, volume = {20}, Number = {5}, pages = {94-102}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-932-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-932-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {fazeli, zeynab and fazeliebavandpor, fatemealsadat and abdi, alirea and porhoseyngholi, mohamad amin and taghinejad, hami}, title = {}, abstract ={}, Keywords = {}, volume = {20}, Number = {5}, pages = {103-111}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-934-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-934-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {dadok, m and beglarian, m and mehrabian, s and zali, h and zamanianazodi, m and salehi, m and maleki, mh and zargar,}, title = {Phylogenetic identification of nitrogen-fixing bacteria isolated from the rhizosphere of asparagus plants using 16s rRNA and the effect of zinc on isolated strains}, abstract ={Background: According to the role of microorganisms in nitrogen stabilizer in the fertility of the soil and growth and development of plants, the use of chemical fertilizers zinc sulfate (ZnSo4) as micronutrient index in agricultur. Due to the toxic effects of zinc metal, the aim of this research, isolation and identification of nitrogen stabilizer microorganisms of asparagus rhizosphere and the effect of toxic zinc metal on the growth of bacteria. Material and Methods: In order to isolate microorganisms in nitrogen stabilizer, soil samples from the asparagus rhizosphere preparation in vitro lacks a nitrogen with a temperature of 30 ° c for 48 hours, was cultured, phylogenetic identify it using 16s rRNA do accepted and the MIC strain isolated in different concentrations of zinc metal was determined. Results: Rhizosphere soil samples from the plant among the asparagus a strain of bacteria was isolated, check the phylogenetic it using 16s rRNA species of Acinetobacter calcoaceticus was detected and isolated in disallowed MIC concentration 52.32 ppm zinc metal was evaluated. Conclusion: One of the rhizosphere nitrogen stabilizer strains in the plant asparagus, bacterium Acinetobacter calcoaceticus, increase the intake of zinc metal was microorganisms cause damage and the maximum allowable amount of zinc metal in this review, the concentration is less than the 52.32 ppm.}, Keywords = {Nitrogen fixation, Plant asparagus, 16s rRNA, Zinc sulfate (ZnSo4) }, volume = {20}, Number = {5}, pages = {112-120}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-933-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-933-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {dovlat, e and hasanzadeh, h and RezaeiTavirany, m and semnani, v and jabariarfaee, a and seyyedi, s and jafarzadeh, l}, title = {Evaluation of radio-sensitizing effect of TiO2 nanoparticles on human gastric cancer cell line}, abstract ={Introduction: Nowadays, cancer is the second cause of mortality in the world. Gastric cancer has a high mortality rate (about 70%) which is more common in men and has a higher incidence in eastern Asian countries. One of the methods for cancer treatment is radiotherapy, in which to enhance the efficiency of radiation therapy some of radio-sensitizer agents can be used to enhance tumor cell radiosensitivity. Some nanoparticles can be considered as a sensitizer because of enhancing the cytotoxicity to oxidative stress and increasing free radicals especially ROS within cells, resulting to cell death. In this study, synergistic effect of TiO2 nanoparticles as a radio-sensitizer agent was evaluated in the presence of cobalt-60 gamma rays on human gastric cancer cell line. Materials and Methods: After cell culture, MKN-45 cells were exposed to 2 Gy of radiation and 30 µg/ml concentration of nanoparticles. Viability was calculated using MTT assay. Findings: Exposing cultured cells to nanoparticles alone resulted to a mean decrease of 70% in cell survival. Viability of cells in presence of gamma radiation and nanoparticles was significantly reduced compared to the viability of cells exposed only to radiation or nanoparticle, alone. The effect was dependent both on the nanoparticle crystal type and concentration. Discussion & Conclusion: Nano-TiO2 increased the sensitivity of gastric cancer cells to gamma radiation, due to an increase in the ROS production and cytotoxicity. Anatase crystals have more severe effects than rutile crystal because of having a larger surface area and creation of more free radicals. Therefore, this nanoparticle has the potential to be used as a radio-sensitizer.}, Keywords = {nano-TiO2, Cobalt 60, gastric cancer, radiosensitivity, radiotherapy}, volume = {20}, Number = {5}, pages = {121-128}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-1008-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-1008-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {zali, h and rezaeetavirani, m and salimian, j and ovlad, gh r and bastaminejad, s}, title = {Gene Expression Networks to Analysis DNA Microarray Data}, abstract ={Unlike the reductionist views of classical biology, holistic approach in biology is shown with an explosion in the development of high-tech techniques to produce large amounts of data. Now the challenge for biologists is to discover ways to analyze this data in order to ability to support understanding the complex dynamic systems of life. In recent advanced technologies that are more public, DNA microarray are most famous. Microarray simultaneously examines expression levels of thousands of genes and provides a snapshot of the transcriptional activity of the cells in multiple conditions. Microarray have provided chance , especially in search of new territory, to describe the genes involved in biological processes such as cell cycle, growth and development of cell, assessment of chemical and genetic disorders and to identify genes associated with various diseases. Sheer volume of data produced by microarray studies need to develop advanced statistical analysis computer tools. In this study has been reviewed statistical methods based on graph theory. Construction the gene expression network (GCN), GCN integration with other data, GCN analysis and application GCN for cancer research fully described. Finally we can say that study of the genome through microarray includes more samples and is possible in wide range of the species and in future applications of meta-analysis methods to integrate large amounts of data such as network alignment may help to clarify the similarities and differences between a wide range of species, tissues and disease states.}, Keywords = {Microarray, Gene expression network, Network analysis}, volume = {20}, Number = {5}, pages = {129-137}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-935-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-935-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {fayazfar, s and zamanyanazodi, m and heydarikashel, saeyd and azizyejalileyan, fary}, title = {Evaluation of the Ctytotoxic Effect of Cuminum cyminum essential oils on Human Colon Cancer cell line SW742}, abstract ={Abstract Background and aim: Colon cancer is the second lethal cancer after lung cancer. Due to the existence of many side effects and problems in the ways of cancer treatment arising from surgery, chemotherapy or radiotherapy at the present, the need for such ways to be replaced and new drugs is prominent. Usage of herbal essential oils as drug contributes to a major part of traditional and Islamic medicine. This project aims at studying of anticancer activity of Cuminum cyminum essential oils on human colon cancer cell line (SW742). Materials and Methods: Essential oils are provided by distillation method and their cytotoxicity effects on SW742 cell line were investigated. Human fibroblast cell was used as healthy and control cells. The cells were cultured and different concentrations of each essential oil were induced to cells. After 48 h incubation, cell survival with MTT assay was studied via the statistical methods (software SPSS), averages and standard deviations were achieved and diagrams were drawn. Results: Findings indicate that essential oil affects on cell survival by two different ways evaporation part reduces the cell growth enormously of both cancer and fibroblast cells. Soluble part, on the other hand, stimulates cell growth of fibroblast cells, while reduces cell growth of cancer cells. Conclusion: Analytical data provide considerable information about pharmaceutical properties of this plant. Even though, more evaluations are needed to investigate the exact effects of this plant.}, Keywords = {Keywords: Essential oils, Colon cancer (SW742), Herbal drug, Cuminum cyminum, Cell survival, Anti cancer}, volume = {20}, Number = {5}, pages = {138-148}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-938-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-938-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {hamidi, m and mosavinasab, s d and ahmadi, n and basati, gh and avlad, gh r and zargar, m}, title = {Synthesis of antimicrobial peptides in bacteria}, abstract ={Antimicrobial peptides -AMP- are diverse group of antimicrobial compounds, which are due to the emergence of pathogenic bacteria resistant to common antibiotics, are of great interest. These molecules like proteins often contain amino acids that are modified by deformation and the polypeptides made by ribosomes are not found. These compounds include both bacteriocin and peptide antibiotics, which are classified based on their mechanism of biosynthesis. Bacteriocins are synthesized by ribosomes and active against bacteria are closely related. Antimicrobial peptides are not synthesized by ribosomes and synthesized during the reaction compression stage with a non-ribosomal peptide complex large (NRPS). The main obstacle to the use of AMP as antibiotics is the lysis of eukaryotic cells. As they apply to pharmaceutical agents must also have a high antimicrobial activity, have low hemolytic activity. Other obstacles such as other toxicity, high cost of production, poor bioavailability and so on should also be considered. Multiple host systems -vector to produce AMP using recombinant DNA technology has been used that the E.coli had the highest use. Thus, there is great potential in the field of antimicrobial peptides and further research in this area can lead to excellent results that have significant effects on food and medicine}, Keywords = {peptide, antimicrobial, bacteria}, volume = {20}, Number = {5}, pages = {149-157}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-936-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-936-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {ranjbar, sm and mosavinasab, sd and ahmadi, n and basati, gh}, title = {Promising therapeutic approaches for Cancer lying on unique features of Apoptin protein from chicken anemia virus (CAV)}, abstract ={Cancer is still a major cause of death in humans. The novel approaches to tumor treatment are targeted therapies that increased hope for improving the treatment of therapy resistant tumors. By relying on new human understanding of the molecular pathogenesis of cancer we can then development of targeted novel drugs such as Nanobodies and monoclonal antibodies, small molecule inhibitors, mimetic, antisense, small interference RNAbased strategies and design and application of oncolytic virotherapy approaches. Tumor-specific killer proteins could derive from both human and animal viruses are such as E4ORF4 and VP3 (apoptin), E1A, viral protein R (VpR), Brevinin-2R, Large T protein, TTV apoptosis-inducing protein (TAIP). Chicken anemia virus (CAV) was isolated in 1979 by Yuasa (Japan), in commercial poultry. CAV is a resistant and ubiquitous virus belongs to the Gyrovirus genus, Circoviridae family and has a circular single strand DNA. It infects and cause disease in young chickens and immunosuppression in all birds. Hematopoietic stem cells in the chicken bone marrow and T-cell precursors in the thymus are the major targets for this virus infection. Apoptin (or VP3) is a small protein (14 kDa) encoded by VP3 gene of CAV. It has attracted great attention because of its tumor-selective toxicity potency means induces of cell death specifically in human transformed or tumor cells. In normal cells, Apoptin remains in the cytoplasm whereas in cancerous cells, it translocates into the nucleus and kills the cell. By nuclear localization of Apoptin, it mediate apoptosis and this mechanism is p53-independent and cannot be blocked by Bcl-2 .This article aims to present a current applications and novel progresses of using apoptin as tumor-specific killer agent and will provide an insight into the possible mechanism of action. Finally we will suggest a new direction for the therapeutic use of viruses and viral proteins for treatment of different kinds of Cancers.}, Keywords = {Cancer cell, chicken anemia virus (CAV), Apoptin, tumor-specific killer protein}, volume = {20}, Number = {5}, pages = {158-167}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-939-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-939-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {nilsaz, mahnaz and tavasoli, elahe and mazaheri, maryam and sohrabi, fereshte and khezeli, mehdi and ghazanfari, zeynab and a, mirzaei}, title = {Study of Health-promotion behaviors and Life Style among students of Dezful universities}, abstract ={Background & Objective: Health promoting activities and a healthy lifestyle are major strategies to preserve health. Regarding the importance of health in young people, health promotion in society a, this study was designed to Study of Health-promotion behaviors and Life Style among students of Dezful universities Materials & Methods: This study was a cross-sectional-descriptive survey assessing health-promoting lifestyle among students of Dezful universities. A total number of 440 students were recruited in this study using a randomly sampling method. Data were collected using a questionnaire (demographic questions, Health-promotion behaviors including physical, social and emotional functions). Obtained data analyzed by SPSS software in two categories of descriptive and inferential statistics (T test, ANOVA). Results: Age range of participants was 22/78. In addition, 51/1 % of the subjects were BS, 33/7 resident in dormitory, 17% married, 11/3 % of the students had a week Life Style, 54/3% moderate and 34/3% good. Statistical significant association was seen between Life Style and mother job, physical function, Restrictions of Role Playing in Condition of emotional problems and bodily pain (p<0/05). Conclusion: The results reveal that the life style of more than half of the students is week and moderate and students have lower physical and emotional function, thus need designing programs for improving university student's Health-promotion behaviors.}, Keywords = {Keywords: Health-promotion behaviors, life style, students}, volume = {20}, Number = {5}, pages = {168-175}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-940-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-940-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {kashfi, seyed mansoor and khanijayhoni, ali and mohamadkhah, fateme and babaeiheydarabadi, akbar and zainli, m}, title = {A Survey on awareness level of transmission ways of B- hepatitis and Aids among Marvdasht City barbers}, abstract ={Abstract Introduction: HIV-Aids and hepatitis, B are the global problems that their destructive effects reduced life expectancies in millions of people. Although in some developed countries a significant success in control of these diseases had seen, but in developing countries still the expanding epidemic continues. One of the causes of prevalence and incidence of these two diseases is lack of the necessary knowledge in society, especially in those who are directly at risk of these diseases. This study aimed to assess Marvdasht barbers awareness in the field of transmission and prevention of Aids and hepatitis B. Materials and methods: this survey is a cross- sectional study.The study population include 180 Marvdasht barbers (110 males & 70 females). Data collection tools consisted of two parts: questions on demographic characteristics and questions related to knowledge about Aids and hepatitis B. SPSS soft- ware were used to analyzed data for Anova and correlation coefficient. Results: Mean age was 29years, it showed participating to the classes and subjects ages had no significant relation ship, but the level of education showed a significant relationship with knowledge about AIDS, but not significant in hepatitis B. 76 percents of the subjects had a good knowledge of AIDS and 58 percent of people were also aware to hepatitis B.}, Keywords = {Key word: awareness, AIDS, hepatitis B, Health education}, volume = {20}, Number = {5}, pages = {176-184}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-943-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-943-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {vahabi, n and gohari, mr and zayeri, f and salehi, m and nasrollahi, a and haidarimighadam, r and javaheri, m}, title = {Title: Application of Longitudinal Latent Regression Model (LLRM) for evaluating the Quality of Life (QoL) in Blind War Veterans}, abstract ={Introduction: QoL can be an important index for determining the social status and performance evaluation of presented services. The main goal of this article is to show the application of longitudinal latent regression model for evaluating the quality of life and assessing the effect of time and other covariates. Materials and Methods: This was a longitudinal study under 71 blind veterans. Under study population includes all blind war veterans that their data collected from SF36 questionnaire with assist of Foundation of martyrs and veterans affair during 2007, 2009 and 2011. SAS/9.1.1 software was used to analyze the data set. Findings: Mean age and blindness long range (±SD) devotees were 48.97 (±10.655) and 25.74 (±3.692), respectively, also they have 2 average additional ulcers with their blindness. Age and length of blindness except than additional disability (0.046) were not statistically significant and the QoL has not meaningful change during the study time. Also variance component of the model were statistically significant. Discussion and Conclusion: Result of this article shown that approximately half of devotees had low QoL. Whereas there was no significant time effect but the QoL of blind war in second and third year were lower than first year.}, Keywords = {Quality of Life, Blind War Veterans, Rasch Model, Longitudinal Latent Regression Model}, volume = {20}, Number = {5}, pages = {185-196}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-941-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-941-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {zare, s and shabani, n and sarsangi, v and babaeehaidarabadi, a and aminizade, r and arabparizi, v and abbasi, am}, title = {Investigation of the Safety Climate among Workers in Sirjan GolGohar Mining and Industrial Company}, abstract ={Mine working environment has been considered one of the most high risk environments. 85 per cent of the work accidents are attributable to unsafe working conditions. 85 to 95 per cent of these accidents are attributed to attitudes, culture and behaviour. The safety climate is a multi-faceted construct which shows the individuals’ attitudes towards and priority they set upon the safety in working environment. Measuring safety climate provides a glimpse of safety condition in an organization in a given time. The present research is an attempt to study safety climate and its structural aspects in Gol gohar mine complex in Sirjan, Iran. The cross-sectional and descriptive-analytical research has been carried out during the winter of 2012on 534 workers in the Sirjan Gol Gol gohar mining and industrial company as subjects. The safety climate questionnaire has been used to measure the safety climate. The findings indicate that production pressure with the score of 5.38 was the least effective factor, and training with the score of 7.70 was the most effective factor. The total score of safety climate has been calculated in a 1 to10 scale for the 6.35 score location. The safety climate of the mine has been shown to be moderate.}, Keywords = {safety climate, management commitment, accident, Gol Gohar}, volume = {20}, Number = {5}, pages = {197-206}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-942-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-942-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {haydarikashl, s and biazar, e and karimi, a and asefnejad, a and amirkhani, a and shahriarinour, m and kazembegi, f}, title = {Smart Hydrogel designed PNIPAm / chitosan by Gamma Irradiation of Biological applications}, abstract ={The abrupt conformational matrix, in which PNIPAM or its modified forms transition of it upon changes at temperatures, may be embedded. It is expected that the change around 32´C has stirred up explorations for in temperature may induce the conformational technological applications. Methods & Materials: Thermo-sensitive hydrogels were prepared by graft copolymerization of chitosan and N-Isopropylacrylamide via gamma radiation. Characterization of hydrogels such as 13C-NMR, DSC analysis and swelling test and cell assessments for harvesting living cell sheet were investigated. 13C-NMR and DSC analysis showed chitosan and NIPAAm monomer were grafted via gamma radiation successfully. Findings: Swelling ratio and curves results administrated hydrophilicity / hydrophobicity of hydrogel that this property is due to presence of PNIPAAm in different temperatures. The hydrogel was tested for harvesting epithelial cells after carrying out cell culture at 37 °C and incubating the confluent cells at 4°C for spontaneous detachment of cell sheet from hydrogel surface without enzyme treatment. Discussion & Conclusion: Cell viability assay results and microscopic observations demonstrated that cells could attach to the hydrogel surface and maintain high viability and proliferation ability. Cell detachment efficiency from the hydrogel was high. These unique properties of the hydrogel would make it a promising support for epithelial cell grafting especially cornea regeneration.}, Keywords = {Tissue engineering, intelegent polymer temperature response, chitosan, poly N-Isopropylacrylamide}, volume = {20}, Number = {5}, pages = {207-215}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-945-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-945-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {zali, h and rezaeetavirani, m and seiedkhaninahal, a and shahriarinoor, m and bolghari,}, title = {Data Analysis of Two Dimensional Electrophoresis Gels by Multivariate Statistical Methods}, abstract ={Background: In recent years technology has emerged to describe the protein spots on two-dimensional gel electrophoresis. Numerous gel statistical analysis software has been developed and the impact of these initiatives on promoting proteomics is impressive. Proteomic analysis of massive data sets with having high variable need multivariate methods to provide the simultaneous analysis of multiple variables. In this study, the process of cellular differentiation and aging of stem cells to astrocytes studied by proteomics and gel analysis software and statistical applications were considered. Materials and Methods: Proteome of four groups (stem cells, young astrocytes, moderately differentiated astrocytes and old astrocytes) were analyzed by the software of Progenesis Same Spots. Cluster analysis, principal component analysis and analysis of power were used in the experimental groups. Results and Discussion: In bioinformatics and statistical analysis of two-dimensional gel electrophoresis technique were detected 940 protein spots with significant expression changes (p <0.05) in four groups that comparisons between groups suggest that the expression of new proteins and the silencing of certain proteins in the signaling pathway of cell differentiation and senescence. Clustering analysis of the expression of proteins can be divided into two main clusters indicate that there are clusters of proteins with similar expression which these proteins can provide similar performance in terms of testing or indicate its presence in the same biological pathway. PCA analysis confirmed the clustering results showed that the protein has been classified according to the test conditions. Finally, we can conclude that using statistical analysis software to quickly and easily done and significant expression changes induced differentiation and senescence on proteome level as well as evaluated with statistical analysis of clustering and PCA and determined indicators of changes.}, Keywords = {Two-dimensional gel electrophoresis, Multivariate statistical technique, Protein}, volume = {20}, Number = {5}, pages = {216-224}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-947-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-947-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {zali, h and rezaeetavirani, m and haidarbegi, k and shahriarinoor, m}, title = {Network Analysis Methods for Interpreting Complex Phenotypes in Biological Networks}, abstract ={Gene network analysis is an important part of systems biology studies. Compared with traditional genotype/phenotype studies that focused on establishing the relationships between single genes and interested traits, network analysis give us a global view of how all the genes work together properly, which in turn leads to the correct biological functions. Network analysis also helps to derive useful information from the network and also helps the discovery of biological processes from a network. In this study, the main methods and applications in network analysis to interpret complex phenotypes basically explain three aspects. The first aspect is to identify the importance of each node in the network which determine more important or crucial genes, or less important or dispensable one. Another aspect is to identify which genes are more functionally related through the whole network view by measuring the direct gene connections and also by considering the connections through the whole network. Identifying the paths or flows through the networks with known input and output genes is the last aspect discussed in network analysis. Although these methods have many advantages, network biology still faces many challenges so more methods have emerged, which provide important tools for network analysis. Mastering these methods is necessary, but far from sufficient for understanding biology. More important things to do are to ask the right questions, to choose proper network analysis tools, and to validate analysis results by solid experimentation. Finally can express that the fundamental goal is the same for network biology and molecular biology – to better understand biological processes and the mechanisms of human diseases.}, Keywords = {Node, Edge, Gene expression networks, Network analysis}, volume = {20}, Number = {5}, pages = {225-233}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-948-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-948-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {haydarikashl, s and rezaeetavirani, m and ebrahimi, m and solaimani, m and roozafzoon, r and kaviani, s and behroozi, gh r and mohammadpour, sh and moayeri,}, title = {Ability of conservation embryonic stem cells, umbilical cord blood mesenchymal stem cells as a feeder layer}, abstract ={Embryonic stem cells (ES) are derived from the pluripotent inner cell mass (ICN) cells of blastocysts with the potential to maintain an undifferentiated stat indefinitely. The derivation process involves plating of the blastocysts on mouse embryonic fibroblast (MEF) and expansion of the outgrowth in to established ES cell line. ES cell capable of unlimited self-renewal by symmetric division and differented cells to all primitive embryonic germ layers. The capacity of ES cells to differentiate in to almost all the cell types of human body highlights their potentially to play a promising role in cell replacement therapies for treatment of human diseases. Materials & Methods: In this study, MEFs has been replaced with human mesenchymal stem cells (hMSCs). C4 mES cell (mouse embryonic stem cell line) colonies culture on inactivated hMSCs amplified ≥ 600-folds during the 30-days of continues culture. The longest continues expansion of C4 mES cells on hMSC was 30 passages. Finding: In this study the gene expression for Oct-4, Nanog, Rex1, Brachyury, LIF, LIFR, TERT, B2M, Stat3, Sox2, Fgf4 in mES cells, using reverse transcriptase polymerase chain reaction (RT-PCR) and in which genes expression for Stat3, Sox2, Fgf4 genes was negative whilst the genes expansion for Oct-4, Nanog, Rex1, Brachyury, LIF, LIFR, TERT, B2M genes was positive. There was also a karyotype analysis for ES which showed normal result. The immunocytochemical analysis of Oct4 transcriptional factor for ES cells was made which showed positive result for this factor. Discussion & Conclusion: These genes may be novel candidates to play critical roles in the regulation of ESC pluripotency and self-renewal.}, Keywords = {Embryonic stem cell, mesenchymal stem cell, blastocysts, feeder layer, pluripotency}, volume = {20}, Number = {5}, pages = {234-242}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-950-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-950-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {haydarikashl, s and rezaeetavirani, m and ebrahimi, m and roozafzoon, r and frozanfar, f and jahani, h}, title = {Isolation of Adherent CD 133 Positive from Cord Blood Unrestricted somatic stem cell its rare population and Nominate as a Valuable Source to Tissue Engineering}, abstract ={Introduction: Stem cells have the ability repair damaged tissues are too. Mesenchymal stem cells from tissues such as bone marrow, peripheral blood and other tissues have been separation. CD133-positive stem cells have Pluripotency properties and are powerful candidates for application in cell therapy. Materials & Methods: In this experimental study, MACS kit was used to isolate CD133+ cells of umbilical cord blood. CD 133 positive cells were evaluated by flowcytometry for surface stem cell marker such as CD 90, CD34, and CD105 and than karyotiping are performed. Finding: Flowcytometry results showed that approximately 95% of purified cells were CD133+. Heterogeneous cell populations have positive level of CD105: 78.35, CD90: 82.32 and then CD34: 8.56. In CD133 positive fraction, expression of surface marker was: CD105: 55.63, CD90:43.25, CD34: 18.2.This cells were Normal karyotype and Spindle shaped like Mesenchymal stem cell. Conclusion: The study results showed that the mentioned CD133 positive cells combination and alone can be act a Nominate as a valuable source to tissue engineering. Keywords:}, Keywords = {CD133+ cells, USSC, Flowcytomtry, Stem cell,}, volume = {20}, Number = {5}, pages = {243-254}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-951-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-951-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {haydarikashl, s and tahermansori, h and atghaee, m and biazar, e and saifipour, f and avazpour, m and kazembegi, f}, title = {Evaluation of Multi-walled Carbon Nanotubes Cytotoxicity Elements Oxathiazole and Phenyl hydrazine Derivative was prepared by Microwave}, abstract ={Abstract: introduction: Since carbon nanotubes (CNTs) were discovered by Iijima in 1991, it opened up new opportunities in the field of molecular electronics, sensoring, composite materials, drug delivery system and cancer therapy. Application of CNTs in drug delivery systems is being investigated actively because of their useful combination of size and physicochemical properties. Matherials; Methods: Significant factor in our study, multiwall carbon nanotubes by chemical carboxyl labeled (MWNT-COOH) with methyl 2 - (2 - amino - 4 oxo Thiazole - (H4) - Alidin) acetate ((MWNT-Amide in terms of microwave said. then, carbon nanotubes amide labeled (MWNT-Amide) by reaction with phenyl hydrazine in 20 minutes derived Thiazole on nanotubes produced by spectroscopy IR, Raman, SEM, elemental analysis, and TGA was Also examined the effect of the nanotubes on cells. Finding: Results are highly toxic to cells than other samples of MWNT-Thiazole showed. Discussion & Conclusion: fictionalization of groups is very effective for toxicity reduction of MWNT that can be used for conjugate to drugs or biochemically materials.}, Keywords = {Nanotubes, Phenyl hydrazine, Microwave, Cytotoxicity}, volume = {20}, Number = {5}, pages = {255-264}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-952-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-952-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} } @article{ author = {zali, h and rezaeetavirani, m and seiedkhaninahal, a and moradi, sh}, title = {Application of Non Linear Statistics (Principal Component Analysis) in Data Analysis of Differentiation Stem Cell to Astrocyte}, abstract ={Background: The combination of univariate and multivariate statistics can identify significant biological changes in protein expression between experimental groups. One of the most common statistical methods that help to analyze two-dimensional gel electrophoresis is principal component analysis. In this study, the differentiation of stem cells to astrocytes is study by proteomics and cell proteome of two groups will be analyzed by principal components analysis (PCA) by statistical software. METHODS: Bone marrow aspirates from healthy donors and isolated mononuclear cell. Cells in 10% DMEM with low glucose, glutamine, streptomycin and penicillin in CO2 5% and moisture 98% were incubated at 37 º c. For differentiation of these cells into astrocytes, cells exposed to retinoic acid, cAMP, PGF, PDGF, NGF. Stem cells and astrocyte cell proteom were extracted and separated by two dimensional electrophoresis. The gels were stained using silver staining and scanned gels were analyzed statistically by using the Bioinformatics analysis software. Results and Discussion: Bioinformatics and statistical analysis of two-dimensional gel electrophoresis technique is shown that 774 protein spots were detected in the two groups. Comparisons between groups suggest that the expression of new proteins and the silencing of certain proteins in the signaling pathway of cell differentiation. Clustering analysis of the expression of proteins can be divided into three main clusters indicate that there are clusters of proteins with similar expression which these proteins can provide similar performance in terms of testing or indicate its presence in the same biological pathway. PCA analysis confirmed the clustering results showed that the protein has been classified according to the test conditions. Finally, we can conclude that the differentiation makes a significant change in the level of expression of the proteome and statistical analysis like clustering and PCA can be considered as good and revealed indicators of changes.}, Keywords = {Proteomics, Principal components analysis, Astrocytes}, volume = {20}, Number = {5}, pages = {265-273}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-953-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-953-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2013} }