@article{ author = {karami, ali}, title = {Evaluation of LAMP (Loop-mediated isothermal DNA amplification) for Molecular Detection of Salmonella}, abstract ={Introduction: Salmonella Bacterium is not only a causative factor of typhoid fever, enterocolitis, and salmonellosis, but it is also a zoonotic infection. This bacterium is a major health problem throughout the world, and is especially prevalent in developing countries. Therefore, rapid diagnosis of salmonella can prevent its outbreak. Different techniques are used for the diagnosis of Salmonella bacteria, such as culture, biochemical, serological, ELISA, Widal, immunofluorescence and molecular methods like PCR and Real time PCR,all of which are difficult, time-consuming, and expensive. Thus, our study was designed to evaluate the LAMP (Loop-mediated isothermal DNA amplification method) for detection of salmonella bacteria. Materials & Methods: In this study, we examined 7 different strains of salmonella. The DNA was extracted by standard methods and amplified with specific primers by PCR and set of primers for LAMP in single temperature in very simple thermal block made in Iran. The amplified products were detected by gel electrophoresis and LAMP products were visualized by their turbidity with naked eye. Findings: Conventional PCR method for detection of Salmonella needs standard thermocylcer and takes 3 hours, but using LAMP method, we were able to amplify and detect salmonella in simple thermoblock, taking much less time. After optimization of the process, it was, soon, possible to detect and identify Salmonella typhi bacteria within 90 minutes. This method is also 10 times more sensitive than that of the PCR. Discussion & Conclusion: According to the results, comparing LAMP method for detection and identification of Salmonella with conventional PCR, we have been able to determine the simplicity, speed and the superior sensitivity of the LAMP method. This Method is more simple, faster and cheaper. Non-dependence of cycle's temperature and thermo-cycling and replacement with one thermo block which is very simple, inexpensive and made inside the country, can be considered another advantage of the LAMP method.}, Keywords = {Salmonella, molecular detection, PCR, DN, LAMP}, volume = {17}, Number = {3}, pages = {1-10}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-144-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-144-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2009} } @article{ author = {Abtahi, hami}, title = {Effects of Micro-filter in Detection of Coliform in Tap Water by PCR}, abstract ={Introduction: For detection of E.coli in top water by PCR, the samples must be concentrated by filter. In this study, we investigated two kinds of micro filters in PCR results. Materials & Methods: After preparing dilution of E.coli (8/100, 4/100, 2/100, 1/100, 1/200, 1/400, 1/800 and 1/1600) in D.W, the bacteria were separated by FHLP and HAWP filters and 16s rRNA was propagated by the above- mentioned primers. Findings: The rate of bacteria in dilutions was confirmed by culture. The PCR data showed that FHLP, better than HAWP filter, would be able to separate the bacteria in such dilutions after filtration. Discussion & Conclusion: The results of this study showed that hydrophobic filter (FHLP) has a higher ability than that of hydrophilic filter (HAWP) in separating bacteria. So, successful PCR amplifications were achieved by cells concentrated with hydrophobic filters for detection of all the coliform bacteria, while false negative results decreased considerably.}, Keywords = {coliform, PCR, microfilter }, volume = {17}, Number = {3}, pages = {10-15}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-145-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-145-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2009} } @article{ author = {baluchi, rami}, title = {Physical Fitness of The Children Infected With Cerebral Paralysis Through A work-group of}, abstract ={Introduction: Studies show that the poor movement in kids leads to paralysis which in turn affects their growth in general. Movement is an important element in the natural growth and development of human beings. Children who lack movement capabilities are deprived of this merit. Materials; Methods: The samples consisted of 37 teenagers (boys & girls) infected with diplegia spastic. They were assigned into two groups based on thrombly CA test (1983), one experimental group and one control group. Different instruments were used in the study such as thrombly CA, an attitude questionaire for the parent's Shtrass Myrse and the dynamometer approach. Findings: Statistical analysis of data before and after the investigations demonstrated of significant differences in test results of Thrombly both in the experimental and control groups. Besides, significant differences were seen in test results out of the questionnaires provided by parents in the experimental group but not in the control group. Self-help skills proved meaningful in experimental groups than the control groups, while significant development of power tiller in left and right hands in both the groups were indicative. Disussion & Conclusion: Through training the therapists, the kids and the parents, one can improve the levels of skills among the given subjects. Also, the confidence level of the parents can be boosted. The results seemed to be consistent with the findings reported in rehabilitation centers.}, Keywords = {Physical Fitness of The Children Infected With Cerebral Paralysis Through A work-group of }, volume = {17}, Number = {3}, pages = {16-24}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-146-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-146-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2009} } @article{ author = {hemati, karim}, title = {Cancer Incidence in Ilam}, abstract ={Introduction:In early 21 st century, cancers are Gnsidered of the most important causes of mortality in the world. More than 30/000 parts and 70/000 new cases are reported in Iran annually .As cancer has an important impact on the quality of life, itsprevention will be one of the most important responsibilities of health care providers and managers .Today, cancer registeration is considered a part of cancer control program. Materials & Methods:The necessary data was collected by trained health care providers from pathology and health centers in Ilam and neghbouring provinces. Findings: Based on the findings of this study, the population of all the known cases in Ilam(western-Iran) during 2001-6 were, 1117 cases. Crude incidence in males and females were 239.3 and 178.5 in 100/000 respectivly.The most common cancer in men occurred in skin (59/100000), esophagus (28.2), gastric (25.7), hematologic(11), and prostatic(10.5) areas respectively. The most common cancer in female stroke was skin (35.7), breast (28.3), esophagus (26.6), gastric (10.9), uterine (7.06), hematologic(6.2) respectively. Also the highest incidence occurred in the age groups of 70-74 and 60-65 and the lowest incidence occurred inthe age groups of 0-4.S.C.C and adenocarcinoma were the most common pathologied in this study. Discussion & Conclusion: Skin and gastro intestinal cancers (esophagus and gastric) are the most prevalent of such in Ilam (western-Ilam). It seems necessary for all the health care providers to play their important role in screening and teaching programs in prevention of such outbreaks of cancers.}, Keywords = {incidence, caccer, Ilam}, volume = {17}, Number = {3}, pages = {24-32}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-147-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-147-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2009} } @article{ author = {maleki, farajalah}, title = {Association of Glutathione S-transferase And Chromosomal Aberrations As a Means to Determine Occupational Exposure}, abstract ={Introduction: The recognition and therapeutic uses of rays in medicine has drawn attention to its biological effects and dangers for people exposed to it. Researches have shown that cell nucleus and chromosomes are the main targets of damage due to X ray. This damage causes chromosome instability and other damages like ring dicentric and acentric. Chromosome damage is important, because they are related to many diseases including malignancies. On the other han, X ray may cause changes in the activities of enzymes involved in the protection of cell for detoxicating. One of these important enzymes is GST. In this study, the relationship between GST and chromosome disorders in environmental lymphocyte in radiotherapists in governmental hospitals of Tehran was studied in comparison with a control group. Materials and methods: This study was aimed at determining the relationship between GST enzyme activities and the frequency of chromosome aberrations in environmental lymphocyte in radiotherapists in governmental medical centers with a length of service of more than 5 years. 33 radiotherapists including 19 females with an age mean of 31.5±15 and males with an age mean of 37.6±14, having over 5 years of service were the member of the experimental group. 37 of the staff of the same hospitals including 22 females with an age range of 36.8 ± 14 acted as the control group. The conditions for entering the ivestigations were length of occupational expericence over than 5 years, drugs, not using acetaminophen, antibiotics for one month before sampling, not experiencing a blood related disease and not having a background of undergoing X-ray tests. The same conditions were applied to the control group except that they didn`t work in radiotherapy centers since the sample was small, all the conditions were checked. 5ml blood heparinised was taken from both the groups to investigate enzyme activities and chromosome aberrations. The GST enzyme activities and chromosome aberrations were investigated with Habig and banding -tripsin G methods respectively. Finding: The study showed that ring dicentric and acentric chromosome aberrations and GST enzyme activities are significantly more in experimental group than in the control group. (P=0.04) Discussion & Conclusion: The results showed an increase in the GST enzymes activities as well as in the chromosome aberrations due to X-ray ionizations amony the radio the rapists.}, Keywords = {chromosome aberrations, glutathione s-transferase, radiotherapy}, volume = {17}, Number = {3}, pages = {33-41}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-148-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-148-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2009} } @article{ author = {malekshahi, rahim}, title = {Design And Implementation of An Apparatus to Produce The Specific Electrical Signals for Providing Desired ELF Electromagnetic Fields to Survey Therapeutic Effects of Energy Resonance Induction}, abstract ={Introduction: Bioelectric currents in a patient’s body are possible to act abnormal due to medical problems such as damaged tissues, bones, nerves or blood. Frequency specificity of pulsed electro-magnetic energy is one of the most important factors in obtaining maximum benefits for healing of damaged tissues and pain relief. The waveforms, the available harmonics, and the ON-OFF duty cycle must also be considered. Materials & Methods: Our research has focused on design and implementation of an apparatus to provide extremely low frequency fields in the ELF spectrum of magnetic energy, from a specially designed coil, and presented to the body. The frequencies of specific pulse and sine wave are in the range of 1 Hz to 100 Hz, and each has related biological responses at the cellular level. This system has been referred to as Magnetic Energy Resonance Induction Therapy, or ‘MERIT’. Findings: The output signals have maximum amplitude equal to 2.5v, pulse duty 0.1 to 10ms. The Burst-On Time and Burst off Time of the produced signal was between 1 to 10 second and the total time was between 1 to 999 minute. The user can justify the variable inputs by keyboard and the display system in LCD. Discussion & Conclusion: Application in treatment for electromagnetic fields and its importance rather than conventional methods makes it necessary to have a purposeful program to provide and extent facilities for researchers. The first step in this program is to be designing and producting a reliable, inexpensive and delicate apparatus.}, Keywords = {specific signals, ELF, electromagnetic, MERIT}, volume = {17}, Number = {3}, pages = {42-50}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-149-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-149-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2009} } @article{ author = {Mardani, mahnaz}, title = {A Study on The Effects of Rhus Coriaria (Somac) on LDL Cholesterol Level Compared with Lovastatin}, abstract ={Introduction: Ischemic heart disease, more than any other disease, leads to mortality and morbidity and high economical costs in developed countries. A cardiovascular disease, especially atherosclerosis, is predicted to become the most important disease in 2020. A disorder which reduces the quality of life due to early death and deterioration. Today, lipid metabolism and lipoprotein disorders have come to be the most important risk factors of atherosclerosis. So,management and treatment of dyslipidemia, is considered an effective preventive method. Considering the high level of atherosclerosis rate in khorramabad (western-center Iran) as well as several reports on anti oxidative effects of Rhus coriaria , this study was planned to compare the effects of lovastatin and Rhus coriaria in decreasing LDL cholesterol level. Materials & Methods: In this study, 86 patients with elevated LDL cholesterol levels, having the indications to enter the study, were selected and divided into two groups of 43 people. Group 1(control group) underwent treatment with lovastatin, and group 2(case group) took lovastatin+Rhus coriria as their cure. After 3 months, LDL cholesterol levels were measured in both the groups. Findings: 58 patients of all the 86 patients completed the tests. The mean LDL levels in the control and case groups before intervention were 141.82 and 171.52 respectively. After intervention, they were 115.7 in the control and 141.45 in the case group. Due to higher level of LDL in the case group vs. the control one before the intervention, the mean level of LDL decreased and its percentage was measured. The decrease of LDL level in the case group was 17%, while it was 18% in the control cases. Nevertheless, thw mean decrease in case patients was 30.07, while it was 26.12 in the control ones, a difference which was not significant statically. Discussion & Conclusion: Due to insignificant decrease of LDL level in both the groups of patients, using Rhus coriaria (Somac) is not recommended as an effective factor in treating patients suffering from high levels of LDL cholesterol.}, Keywords = {ischemic heart disease, atherosclerosis, lipoprotein, dyslipidemia, Rhus coriaria.}, volume = {17}, Number = {3}, pages = {51-56}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-150-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-150-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2009} } @article{ author = {Akbari-adergani, behrooz}, title = {imipramine, fluorescence detection, liquid chromatography, assay, content uniformity}, abstract ={Introduction: Imipramine is a widely prescribed tricyclic antidepressant. However, the routine control of this drug in assay and content uniformity requires a highly sensitive and precise analytical attitude. The aim of this research was to perform assay and content uniformity in tablet samples by high performance liquid chromatography with fluorescence detection. Materials and Methods: As, nowadays, prescription of imipramine tablets is increasing, it seems necessary to improve their quality control. So, 10 and 50 mg tablets of imipramine were randomly collected from market retailers. The chemical structure and inherent fluorescence of imipramine were a good motivation for performing assay and content uniformity in tablet samples by developing and validation of high performance liquid chromatography and fluorescence detection. These experiments were compared with imipramine standards. Findings: The results showed that imipramine could be determined by high performance liquid chromatography with fluorescence detection within 2 minutes. The detection limits(signal-to-noise ratio=3) for the examined antidepressant was 0.2ng/ml. Assay and content uniformity of the tested products met the requirements stated in accredited pharmacopoeias. Based on the statistical t-test, there is no significant difference between the test and reference products. (p>0.05) Discussion & Conclusion: The results of this study demonstrated that the developed and validated HPLC method with sensitive florescence detection can be used in assay and content uniformity of imipramine. From intra- and inter-day coefficient of variations, it can be concluded that this method have a high accuracy and precision. Very low detection limit, wide dynamic range, appropriate accuracy and precision, ability in separation on a polymeric based PRP column in a short time with sufficient robustness are, certainly, the positive aspects of the developed and validated method.}, Keywords = {imipramine, fluorescence detection, liquid chromatography, assay, content uniformity}, volume = {17}, Number = {3}, pages = {57-64}, publisher = {Ilam University of Medical Sciences}, url = {http://sjimu.medilam.ac.ir/article-1-151-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-151-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, issn = {1563-4728}, eissn = {2588-3135}, year = {2009} }