en Antibacterial properties of copper nanoparticles synthesized by aqueous extract of Oliveria decumbens flowers میکروبشناسی Microbiology پژوهشي Research <div style="text-align: left;"><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Introduction</b>: The production of nanoparticles by biological methods is less hazardous than traditional chemical methods and is also cost-effective. The aim of this study was to synthesize copper nanoparticles using the aqueous extract of <i>Oliveria decumbens</i> flowers and to investigate their antibacterial properties.</span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Materials & Methods</b><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">: </span></span></b>The soaking method was used for extraction. Nanoparticle synthesis was carried out by reducing copper sulfate with the aqueous extract of <i>O. decumbens</i> flowers. The resulting nanoparticles were characterized using spectrophotometric analysis, infrared spectroscopy, and scanning electron microscopy. The antibacterial effects of the extract and the synthesized nanoparticles on <i>Staphylococcus aureus, Bacillus cereus, Escherichia coli,</i> and <i>Klebsiella pneumoniae</i> were investigated using the disk method, and their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were determined.<span lang="FA" dir="RTL" style="font-family:"Times New Roman",serif"></span></span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Results</b><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">: &nbsp;</span></span></b>The aqueous extract of <i>O. decumbens</i> flowers was found to reduce copper sulfate. The produced nanoparticles were spherical, with an average size of 36.93 nm. Although the aqueous extract had no effect on the tested bacteria, the synthesized nanoparticles at concentrations of 2000 and 4000 &mu;g/disk exhibited antibacterial activity against all these bacteria. The MIC and MBC values were determined to be 1000 &mu;g/ml for <i>S. aureus</i> and <i>B. cereus</i> and 2000 &mu;g/ml for <i>K. pneumoniae</i> and <i>E. coli</i>.</span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Conclusion</b><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">: &nbsp;</span></span></b>The results showed that the aqueous extract of <i>O. decumbens</i> flowers acts as a reducing and stabilizing agent for copper sulfate. The synthesized copper nanoparticles exhibited acceptable antibacterial activity against the studied Gram-positive and Gram-negative bacteria.</span></span><br> <br> <br> &nbsp;</div> green synthesis, infrared spectroscopy, scanning electron microscopy, microdilution, nanoparticles 14 28 http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-7921-4&slc_lang=en&sid=1 Katrin Ebrahimi Katrin Ebrahimi katrine.ebrahimi@pnu.ac.ir 9600319475328460050477 9600319475328460050477 No Payame Noor University, Dept of Biology, Tehran, Iran Payame Noor University, Dept of Biology, Tehran, Iran Amirarsalan Kavyanifard Amirarsalan Kavyanifard kavyanifarda@pnu.ac.ir 9600319475328460050478 9600319475328460050478 Yes Payame Noor University, Dept of Biology, Tehran, Iran Payame Noor University, Dept of Biology, Tehran, Iran