Journal of Ilam University of Medical Sciences
مجله دانشگاه علوم پزشکی ایلام
J. Ilam Uni. Med. Sci.
Medical Sciences
http://sjimu.medilam.ac.ir
96
journal96
1563-4728
2588-3135
doi
fa
jalali
1403
12
1
gregorian
2025
3
1
33
1
online
1
fulltext
fa
اثر امواج الکترومغناطیسی با چگالی شار مغناطیسی 6/0 و 2/1 میلیتسلا روی بیان miR-584-5p در ردۀ سلولی سرطان معدۀ انسان
The effect of electromagnetic fields with magnetic flux density of 0.6 and 1.2 mT on the expression of miR-584-5p in gastric cancer cell line
بیوفیزیک
biophysics
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:8pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:.1pt">مقدمه:</span></span></span></span></b> <span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">امواج الکترومغناطیسی بهعنوان یک عامل محیطی میتوانند در پیشگیری یا پیشبرد سرطان نقش داشته باشند. </span></span>MicroRNA<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">ها (</span></span>miRs<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">) تنظیمکنندههای بیان ژن در مرحلۀ پس از رونویسی هستند و بهعنوان انکوژن یا سرکوبگر تومور عمل میکنند. </span></span>miR-584-5p<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""> بهعنوان یک ژن سرکوبگر تومور شناخته میشود و افزایش بیان آن در سرطان معده مشاهدهشده است. در این مطالعه، تغییرات بیان </span></span>miR-584-5p<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""> در ردۀ سلولی </span></span>AGS<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""> تحت تأثیر امواج الکترومغناطیسی با فرکانس بسیار کم (</span></span>ELF-EMF<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">) بررسی شد.</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""></span></span></span></span></span></span><br>
<span style="font-size:8pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:.1pt">مواد و روش­ها:</span></span></span></span></b> <span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">سلولهای </span></span>AGS<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""> به مدت 18 ساعت بهطور پیوسته و ناپیوسته (2 ساعت روشن/ 2 ساعت خاموش) تحت تابش امواج الکترومغناطیسی مختلف قرار گرفتند. بهمنظور ارزیابی تغییرات بیان </span></span>miR-584-5p<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""> از تکنیک </span></span>Real-time PCR<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""> استفاده گردید.</span></span></span></span></span></span><br>
<span style="font-size:8pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:.1pt">یافته­ های پژوهش: </span></span></span></span></b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">این مطالعه نشان داد که بیان میر </span></span>miR-584-5p<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""> تحت تابش امواج الکترومغناطیسی پیوسته با شدت 6/0 (3.7</span></span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Cambria",serif">±</span></span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">0.3، </span></span>P<0.05<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">) و 2/1 میلیتسلا (7.9</span></span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Cambria",serif">±</span></span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">0.4، </span></span>P<0.05<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">) بهطور معنیداری افزایش مییابد؛ همچنین افزایش معنیداری در بیان </span></span>miR-584-5p<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""> تحت تابش امواج الکترومغناطیسی ناپیوسته با شدت 6/0 (2.7</span></span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Cambria",serif">±</span></span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">0.6، </span></span>P<0.05<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">) و 2/1 میلیتسلا (6 </span></span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"Cambria",serif">±</span></span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">0.4، </span></span>P<0.05<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">) در مقایسه با کنترل مشاهده شد.</span></span><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:.1pt"></span></span></span></span></b></span></span></span></span><br>
<span style="font-size:8pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">بحث و نتیجهگیری</span></span></span></span></b><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">: </span></span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">با توجه به اهمیت </span></span>miR-584-5p<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""> و نقش آن در آپوپتوز، تمایز، تهاجم، تکثیر و متاستاز در سرطانهای مختلف و تغییر بیان آن تحت تأثیر امواج الکترومغناطیسی در سلولهای سرطان معده، این </span></span>miRNA<span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""> میتواند یک هدف درمانی با استفاده از امواج مغناطیسی بهعنوان طب مکمل در نظر گرفته شود.</span></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:9pt"><span style="font-family:"Times New Roman",serif"><b><span style="color:black"><span style="letter-spacing:-.2pt">Introduction:</span></span></b> Electromagnetic fields as an environmental factor can play a role in preventing or promoting cancer. MicroRNAs (miRs) are post-transcriptional regulators of gene expression and act as oncogenes or tumour suppressors. miR-584-5p is known as a tumour suppressor gene, and its increased expression has been observed in gastric cancer. This study was done to investigate the expression changes of miR-584-5p in the AGS cell line under the exposure of extremely low frequency electromagnetic fields (ELF-EMF).</span></span><br>
<span style="font-size:9pt"><span style="font-family:"Times New Roman",serif"><b><span style="color:black"><span style="letter-spacing:-.2pt">Materials & Methods: </span></span></b>AGS cells were exposed to different electromagnetic fields continuously and intermittently (2 hours on/2 hours off) for 18 hours. Real-time PCR technique was used to evaluate the expression changes of miR-584-5p. Descriptive and analytical tests were performed using SPSS V.23, and a significance level of 5% was considered.<span style="font-size:12.0pt"><span style="color:#374151"></span></span></span></span><br>
<span style="font-size:9pt"><span style="font-family:"Times New Roman",serif"><b><span style="color:black"><span style="letter-spacing:-.2pt">Results:</span></span></b> The results showed that the level of miR-584-5p increased a lot when exposed to continuous electromagnetic wave irradiation at strengths of 0.6 (3.7±0.3, P<0.05) and 1.2 mT (7.9±0.4, P<0.05). Additionally, a notable rise in miR-584-5p levels was also seen with discontinuous electromagnetic wave irradiation at strengths of 0.6 (2.7±0.6, P<0.05) and 1.2 mT (6±0.4, P<0.05) compared to the control. Also, a significant increase in miR-584-5p expression was observed under discontinuous electromagnetic wave irradiation with intensities of 0.6 (2.7±0.6, P<0.05) and 1.2 mT (6±0.4, P<0.05) compared to the control.</span></span><br>
<span style="font-size:9pt"><span style="font-family:"Times New Roman",serif"><b><span style="color:black"><span style="letter-spacing:-.2pt">Conclusion:</span></span></b> Given how important miR-584-5p is for processes like cell death, development, spreading, growth, and movement in different cancers, along with how its levels change due to electromagnetic waves in stomach cancer cells, this miRNA could be a target for treatment using magnetic waves as an additional therapy.</span></span></div>
ردۀ سلولی سرطان معده (AGS), امواج الکترومغناطیسی, miR-584-5p
Gastric Cancer Cell line (AGS), Electromagnetic field, miR-584-5p
30
39
http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-3305-2&slc_lang=fa&sid=1
Soheila Abdi
Abdi
سهیلا
عبدی
abdi.soheila@gmail.com
9600319475328460049103
9600319475328460049103
Yes
Dept of physics, Safadasht Branch, Islamic Azad University, Tehran, Iran
گروه فیزیک، واحد صفادشت، دانشگاه آزاد اسلامی، تهران، ایران