fa جداسازی و شناسایی هلیکوباکتر پیلوری در افراد مرتبط با حیوانات خانگی باكتري شناسي اختصاصي exclusive bacteriology پژوهشي Research <div style="text-align: justify;"><div style="text-align: justify;"></div><span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><span style="font-size:8pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;"><span style="color:black"><span style="letter-spacing:.1pt">مقدمه:</span></span></span></span></b> <span lang="FA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;">عفونت معده با عامل هلیکوباکتر پیلوری از بیماری‌های رایج در انسان محسوب میشود که از طریق خوردن و آشامیدن منتقل می‌گردد. این باکتری توان استقرار در معدۀ برخی از گونه‌های حیوانی ازجمله سگ و گربه را دارد و در برخی موارد، حیوانات حامل منشأ عفونت برای انسان هستند. این تحقیق باهدف بررسی میزان آلودگی هلیکوباکتر پیلوری در افراد مرتبط با حیوانات خانگی و خود حیوانات خانگی در شهرستان اصفهان انجام شد.</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;"></span></span></span></span></span></span><br> <span style="font-size:8pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;"><span style="color:black"><span style="letter-spacing:.1pt">مواد و روش&shy;ها:</span></span></span></span></b> <span lang="FA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;">بدین‌منظور 115 نمونۀ مدفوعی از صاحبان سگ‌ها و گربه‌های خانگی و سگ‌ها و گربه‌های خانگی اخذ گردید و آزمایش‌های کشت میکروبی و </span></span>PCR<span lang="FA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;"> برای شناسایی هلیکوباکتر پیلوری صورت پذیرفت.</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;"></span></span></span></span></span></span><br> <span style="font-size:8pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;"><span style="color:black"><span style="letter-spacing:.1pt">یافته&shy; های پژوهش: </span></span></span></span></b><span lang="FA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;">نتایج حاکی از آلودگی 36 درصدی صاحبان سگ‌های خانگی، 80 درصدی صاحبان گربه خانگی و 8 درصدی خود سگ‌ها بود. در این بررسی، موارد مثبت آلودگی در گربه مشاهده نشد. در آزمون مربع کای (تست پیرسون) با نرم‌افزار آماری </span></span>SPSS<span lang="FA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;">، ارتباط معنی‌داری میان آلودگی سگ</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;">ها و گربه</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;">های خانگی و آلودگی صاحبان آن</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;">ها نشان داده شد (</span></span>P<0/05<span lang="FA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;">).</span></span><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;"><span style="color:black"><span style="letter-spacing:.1pt"></span></span></span></span></b></span></span></span></span><br> <span style="font-size:8pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;"><span style="color:black"><span style="letter-spacing:-.1pt">بحث و نتیجه‌گیری</span></span></span></span></b><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;">: </span></span><span lang="FA" style="font-size:9.0pt"><span style="font-family:&quot;B Zar&quot;">آلودگی نسبتاً چشمگیر سگ‌ها به هلیکوباکتر پیلوری و احتمال انتقال آن به انسان رعایت بیشتر مسائل بهداشتی را بیش‌ازپیش مدنظر قرار می‌دهد.</span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:11.0pt"><span style="line-height:115%"></span></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:9pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="color:black"><span style="letter-spacing:-.2pt">Introduction:</span></span></b> &nbsp;Gastric ulcer caused by <i>Helicobacter pylori</i> is one of the common diseases in humans, which is transmitted through eating and drinking. This bacterium has the ability to settle in the stomach of some animal species, including dogs and cats. In some cases, the carrier animals are the source of infection for humans. The present study aimed to assess the amount of <i>Helicobacter pylori</i> infection in people having contact with pets, and the pets themselves in Isfahan.</span></span><br> <span style="font-size:9pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="color:black"><span style="letter-spacing:-.2pt">Material & Methods: </span></span></b>&nbsp;To dis end, 115 fecal samples were taken from the owners of domestic dogs and cats and microbial culture and polymerase chain reaction tests were performed to identify <i>Helicobacter pylori</i>.<span style="font-size:12.0pt"><span style="color:#374151"></span></span></span></span><br> <span style="font-size:9pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="color:black"><span style="letter-spacing:-.2pt">Results:</span></span></b> The results indicated that 36% of domestic dog owners, 80% of domestic cat owners, and 8% of dogs themselves were infected. In addition, no positive cases of infection were observed in cats. In the chi square test (Pearson&#39;s test), the infection of domestic dogs and cats showed a significant relationship with the infection of their owners (P<0.05).</span></span><br> <span style="font-size:9pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="color:black"><span style="letter-spacing:-.2pt">Discussion & Conclusion:</span></span></b> The relatively significant infection of dogs to <i>Helicobacter pylori</i> and the possibility of its transmission to humans makes the observation of health care more important more than ever.</span></span></div> هلیکوباکتر پیلوری, انسان, سگ, گربه Cats, Dogs, Helicobacter pylori, Human 66 75 http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-7599-1&slc_lang=fa&sid=1 Farnaz Soltani فرناز سلطانی farnaz.s.1997@gmail.com 9600319475328460048057 0009-0005-1919-3265 No Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran دانشکده دامپزشکی، دانشگاه آزاد اسلامی، شهرکرد، ایران Ali Sharifzadeh علی شریف زاده alisharifzadeh10@gmail.com 9600319475328460048056 9600319475328460048056 Yes Dept of Microbiology, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran گروه میکروبیولوژی،دانشکده دامپزشکی، دانشگاه آزاد اسلامی،شهرکرد، ایران