fa شناسایی تاثیر lncRNA MT1JP بر تکثیر، مهاجرت و آپوپتوز سلولی در سلول‌های سرطان ریه بیوتکنولوژی biotechnolohgy پژوهشي Research <div style="text-align: justify;"><span style="font-size:8pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:.1pt">مقدمه:</span></span></span></span></b> <span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">سرطان بیماری پیچیده</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">ای است که در آن بیان ژن</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">ها تغییر می</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">کند. این مطالعه با هدف بررسی تأثیرات</span></span>MT1JP&nbsp; lncRNA<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> در کنترل سلول</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">های سرطان ریه انجام شده است.</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""></span></span></span></span></span></span><br> <span style="font-size:8pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:.1pt">مواد و روش&shy;ها:</span></span></span></span></b> <span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">سلول</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">های </span></span>TC-1[JHU-1]<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> با پلاسمید</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">های </span></span>pcDNA3.1(+)-MT1JP<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span>Plko.1-EGFP-PURO-siRNA<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> و پلاسمید خالی ترنسفکت شدند و بیان ژن </span></span>MT1JP<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> و قطعه </span></span>siRNA<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> توسط واکنش </span></span>RT-PCR<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> تأیید گردید. نقش </span></span>MT1JP<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> در مهاجرت توسط آزمون سنجش خراش و آپوپتوز توسط فلوسیتومتری بررسی شد. تغییر بیان ژن</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">های کاندید دخیل در آپوپتوز و تکثیر سلولی (</span></span>Bim<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span>AKT<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span>Bax<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> و </span></span>Bcl-2<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">) توسط واکنش </span></span>q-PCR<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> ارزیابی شد.</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""></span></span></span></span></span></span><br> <span style="font-size:8pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:.1pt">یافته &shy;های پژوهش: </span></span></span></span></b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">نتایج نشان داد که بیان ژن </span></span>Bim<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> در حضور بیان بالای </span></span>MT1JP<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، افزایش معنی</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">داری را نشان داد در صورتیکه با خاموش</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">سازی </span></span>MT1JP<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> توسط </span></span>siRNA<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، بیان ژن </span></span>Bim<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> به</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">طور معنی</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">داری کاهش یافت. بیان ژن</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">های </span></span>Bax<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> و </span></span>Bcl-2<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> در حضور بیان بالای </span></span>MT1JP<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> به ترتیب افزایش و کاهش معنی</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">داری را نشان دادند. در سلول</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">های ترنسفکت شده با </span></span>pcDNA3.1(+)-MT1JP<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، ژن </span></span>AKT<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> کاهش بیان را نشان داد و در مقابل در سلول</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">های ترنسفکت شده با </span></span>siRNA<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، ژن </span></span>AKT<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> افزایش بیان معنی</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">داری داشت (05/0 </span></span>p <<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">). همچنین نتایج سنجش خراش نشان داد که </span></span>MT1JP lncRNA<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> نقش بازدارندگی بر مهاجرت سلولی دارد. داده‌های فلوسایتومتری نشان داد که ترانسفکشن توسط </span></span>pCDNA3.1 (+) -MT1JP<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> می‌تواند سطوح آپوپتوز سلولی را افزایش دهد.</span></span><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:.1pt"></span></span></span></span></b></span></span></span></span><br> <span style="font-size:8pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt">بحث و نتیجه‌گیری</span></span></span></span></b><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">: </span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">مطالعه حاضر نشان داد که </span></span>lncRNA MT1JP<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> باعث مهار تکثیر و مهاجرت سلولی و در عین حال سبب افزایش آپوپتوز سلول‌های </span></span>TC-1[JHU-1]<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> می</span></span><span lang="FA" style="font-size:9.0pt">‌‌</span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">شود. این مطالعه ممکن است به بهبود درک ما از سرطان ریه کمک کند، با این حال نیازمند مطالعات تکمیلی است.</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""></span></span></span></span></span></span><br> &nbsp;<br> عباس دوستی:<a href="https://scholar.google.com/citations?hl=en&user=Y8BOdVkAAAAJ">Google Scholar</a><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:9pt"><span new="" roman="" style="font-family:" times=""><b><span style="color:black"><span style="letter-spacing:-.2pt">Introduction:</span></span></b> Cancer is a complex disease in which gene expression changes. This study was conducted with the aim of investigating the effects of MT1JP lncRNA in controlling lung cancer cells.</span></span><br> <span style="font-size:9pt"><span new="" roman="" style="font-family:" times=""><b><span style="color:black"><span style="letter-spacing:-.2pt">Material & Methods: </span></span></b>TC-1 [JHU-1] cells were transfected with pcDNA3.1(+)-MT1JP, Plko.1-EGFP-PURO-siRNA and empty plasmids, and the expression of MT1JP gene and siRNA fragment was confirmed by RT-PCR reaction. The role of MT1JP in migration was investigated by scratch assay and apoptosis by flow cytometry. The expression change of candidate genes involved in apoptosis and cell proliferation (Bim, AKT, Bax and Bcl-2) was evaluated by q-PCR reaction.<span style="font-size:12.0pt"><span style="color:#374151"></span></span></span></span><br> <span style="font-size:9pt"><span new="" roman="" style="font-family:" times=""><b><span style="color:black"><span style="letter-spacing:-.2pt">Findings:</span></span></b> The results showed that the expression of Bim gene showed a significant increase in the presence of high expression of MT1JP, while the expression of Bim gene decreased significantly with silencing of MT1JP by siRNA. The expression of Bax and Bcl-2 genes showed a significant increase and decrease, respectively, in the presence of high expression of MT1JP. In the cells transfected with pcDNA3.1(+)-MT1JP, the AKT gene showed a decrease in expression, and on the other hand, in the cells transfected with siRNA, the AKT gene had a significant increase in expression (p < 0.05). Also, the scratch assay results showed that MT1JP lncRNA has an inhibitory role on cell migration. Flow cytometry data showed that transfection by pCDNA3.1(+)-MT1JP could increase cell apoptosis levels.</span></span><br> <span style="font-size:9pt"><span new="" roman="" style="font-family:" times=""><b><span style="color:black"><span style="letter-spacing:-.2pt">Discussion & Conclusion:</span></span></b> The present study showed that lncRNA MT1JP inhibits cell proliferation and migration and at the same time increases apoptosis of TC-1 [JHU-1] cells. This study may help improve our understanding of lung cancer, however further studies are needed.</span></span><br> &nbsp;</div> سرطان ریه, MT1JP, سلول TC-1[JHU-1], q-PCR, آپوپتوزیز lung cancer, MT1JP, TC-1 cell [JHU-1], q-PCR, apoptosis 97 109 http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-7294-1&slc_lang=fa&sid=1 Noosheen Samimi نوشین صمیمی noosheen.samimi@gmail.com 9600319475328460047081 9600319475328460047081 No Dept of Biology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran گروه زیست شناسی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران Abbas Doosti عباس دوستی bio.gene84@gmail.com 9600319475328460047082 9600319475328460047082 Yes Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran مرکز تحقیقات بیوتکنولوژی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران Abbas Mirzaei عباس میرزایی dr_amirzaei@yahoo.com 9600319475328460047083 9600319475328460047083 No Cellular and Molecular Research Center, Basic Health Sciences Research Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran مرکز تحقیقات سلولی و مولکولی، پژوهشکده علوم پایه سلامت، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران