Journal of Ilam University of Medical Sciences
مجله دانشگاه علوم پزشکی ایلام
J. Ilam Uni. Med. Sci.
Medical Sciences
http://sjimu.medilam.ac.ir
96
journal96
1563-4728
2588-3135
doi
fa
jalali
1401
12
1
gregorian
2023
3
1
31
1
online
1
fulltext
fa
اثر ترانس سینامیک اسید بر آسیب شناختی، دانسیتۀ سلولی نواحی CA1/CA3 و فعالیت کولینرژیک هیپوکامپ در گونۀ تریمتیلتینی بیماری آلزایمر
Effect of Trans-cinnamic Acid on Cognitive Deficit, Cell Density of CA1/CA3 Regions, and Cholinergic Activity of Hippocampus in Trimethylettin Model of Alzheimer's Disease
بیولوژی
biology
پژوهشي
Research
<span style="font-size:11pt"><span style="tab-stops:319.65pt right 322.35pt"><span style="line-height:97%"><span style="font-family:Calibri,sans-serif"><b> </b><b><span dir="RTL" lang="FA" style="font-size:10.0pt"><span style="line-height:97%"><span style="font-family:"B Nazanin""><span style="color:#1c57ce"><span style="position:relative"><span style="top:-1.0pt"></span></span></span></span></span></span></b></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:.1pt">مقدمه:</span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">مسمومیت ناشی از تریمتیلتین (</span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">Trimethyltin; TMT</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">) با آسیب دستگاه کولینرژیک در هیپوکامپ همراه است. مطالعۀ حاضر با هدف ارزیابی اثر ترانس سینامیک اسید بر زوال شناختی، آسیب سلولی نواحی </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">CA1/CA3</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> هیپوکامپ و سنجش فعالیت استیل کولین استراز (</span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">Acetyl Choline Esterase, AChE</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">) در هیپوکامپ موشهای صحرایی گونۀ بیماری آلزایمر انجام شد.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:.1pt">مواد و روش ها:</span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">در این تحقیق تجربی، تعداد 40 سر موش صحرایی نر بالغ نژاد ویستار در 4 گروه کنترل، </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">TMT+Saline</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">، </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">TMT+CIN20</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> و </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">TMT+CIN40</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> بهصورت تصادفی تقسیم گردیدند. القای گونۀ بیماری آلزایمر با تزریق درون صفاقی 8 میلیگرم بر کیلوگرم وزن بدن </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">TMT</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> صورت گرفت. 72 ساعت پس از تجویز </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">TMT</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">، دوزهای 20 و 40 میلیگرم ترانس سینامیک اسید روزانه به مدت 4 هفته گاواژ شد؛ سپس سنجش حافظۀ کاری، یادگیری اجتنابی غیرفعال، تراکم نورونی در نواحی </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">CA1</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> و </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">CA3</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> و فعالیت آنزیم </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">AChE</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> هیپوکامپ انجام گردید.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">یافتهها:</span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">افزایش معنادار تراکم نورونی </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">CA1/CA3</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">، درصد تناوب حرکتی و بهبود حافظۀ اجتنابی غیرفعال در گروههای دریافتکنندۀ ترانس سینامیک اسید در مقایسه با گروه دریافتکنندۀ نرمال سالین دیده شد؛ همچنین فعالیت آنزیم </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">AChE</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> در هیپوکامپ بهویژه در گروه </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">TMT+CIN40</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">، در مقایسه با گروه </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">TMT+Saline</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">، بهطور معناداری افزایش یافت.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">بحث و نتیجهگیری:</span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">بهنظر میرسد ترانس سینامیک اسید با آثار مهارکنندگی </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">AChE</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> سبب بهبود زوال شناختی و آسیب سلولی هیپوکامپ در گونۀ تریمتیلتینی بیماری آلزایمر گردد.</span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">Introduction:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif">Trimethyltin (TMT) intoxication is associated with damage to the cholinergic system in the hippocampus. The aim of this study was to evaluate the effect of trans-cinnamic acid on cognitive deficit and cell damage in CA1/CA3 regions of the hippocampus, as well as measure the activity of acetyl choline esterase (AChE) in the hippocampus of a rat model of Alzheimer’s disease (AD).</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">Material & Methods:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif">In this experimental study, 40 adult male Wistar rats were randomly divided into four groups: control, TMT+Saline, TMT+CIN20, and TMT+CIN40. The AD model was induced by intraperitoneal injection of 8 mg/kg TMT body weight, and 72 h after TMT administration, doses of 20 and 40 mg of trans-cinnamic acid were gavaged daily for four weeks. Subsequently, working memory, passive avoidance learning, neuronal density in hippocampal CA1 and CA3 regions, and AChE enzyme activity were measured in this study.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif">(Ethic Code: IR.IAU.SHIRAZ.REC.1400.014)</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">Findings:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif">There was a significant increase in CA1/CA3 neuronal density, percentage of motor frequency, and improved passive avoidance memory in the groups receiving trans-cinnamic acid, compared to the group receiving normal saline. Moreover, AChE enzyme activity in the hippocampus, especially in the TMT+CIN40 group, increased significantly, compared to the TMT+Saline group.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">Discussion & </span></span></span></span></b><b><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="letter-spacing:-.2pt">Conclusion:</span></span></span></b> <span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif">It seems that trans-cinnamic acid with AChE inhibitory effects improves cognitive decline and hippocampal cell damage in the TMT model of AD.</span></span></span></span></span>
سینامیک اسید, دمانس, تریمتیلتین, استیل کولیناستراز, موش صحرایی
Acetylcholinesterase, Cinnamic Acid, Dementia, Rat, Trimethylettin
1
12
http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-6358-3&slc_lang=fa&sid=1
Shima
Mokhtarkia
شیما
مختارکیا
mokhtarkiashim@gmail.com
9600319475328460046319
9600319475328460046319
No
Dept of Biology, Faculty of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran
گروه زیستشناسی، دانشکدۀ علوم، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
Mohammad Amin
Edalatmanesh
محمد امین
عدالت منش
amin.edalatmanesh@gmail.com
9600319475328460046318
9600319475328460046318
Yes
Dept of Biology, Faculty of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran
گروه زیستشناسی، دانشکدۀ علوم، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران