fa بررسی تاثیر لاکتوباسیلوس فرمنتوم بر بیان ژن های Arc و CREB دخیل در حافظه ی رت های آلزایمری فیزیولوژی physiology پژوهشي Research <span style="font-size:8pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:.1pt">مقدمه:</span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">بیماری آلزایمر یک بیماری شایع نوروژنراتیو پیش&shy;رونده است که منجر</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">به زوال عقل و تباهی سلول&shy;های مغزی به خصوص در نواحی مرتبط با یادگیری و حافظه مانند هیپوکامپ می&shy;شود. نقش ژن</span></span> Arc <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">در انعطاف سیناپسی و تثبیت حافظه به اثبات رسیده است و بیان آن به شدت تحت تاثیر فعالیت نورونی است. </span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">هدف از این تحقیق تعیین اثر <i>لاکتوباسیلوس فرمنتوم</i> بر بیان ژن&shy;های </span></span>Arc<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> (موثر در </span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">در انعطاف سیناپسی و تثبیت حافظه</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">) و </span></span>CREB<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> (موثر در تثبیت تغییرات سیناپسی در حین یادگیری) دخیل در حافظه رت&shy;های آلزایمری بود.</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""></span></span></span></span></span></span><br> <span style="font-size:8pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:.1pt">مواد و روش&shy;ها:</span></span></span></span></b> <span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">در مطالعه حاضر که <b>در سال</b> 1399 انجام شد، </span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">30 رت نر نژاد ویستار به </span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">5</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">گروه (کنترل، آلزایمر و3 گروه تیمار) تقسیم شدند. در گروه‌های تیمار هم&shy;زمان با القای آلزایمر به روش استریوتاکسی توسط استرپتوزوتوسین، به مدت 21 روز صاف شده&shy;ی</span></span><i>Lactobacillus fermentum</i> PTCC 1744<span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> در محیط </span></span>MRS<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> براث</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">با دوزهای </span></span>&nbsp;cfu/ml<span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">1</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">0⁶</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">و10⁷ و10⁸ به صورت درون صفاقی تزریق شد. بعد از استخراج </span></span>RNA<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> از</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">نمونه&shy;های هیپوکامپ،</span></span>&nbsp; cDNA <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">سنتز شد و بیان ژن&shy;ها</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">با</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> روش ریل تایم </span></span>PCR<span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> و </span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">نرم&nbsp; افزاز </span></span>LinReg PCR <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">ارزیابی شد</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">.</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""></span></span></span></span></span></span><br> <span style="font-size:8pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:.1pt">یافته&shy;های پژوهش: </span></span></span></span></b><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">داده&shy;ها افزایش بیان ژن </span></span>Arc<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> و </span></span>CREB<span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> را در<i> </i>گروه تیمار با دوز </span></span>cfu/ml <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">10⁶</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> باکتری</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> در مقایسه با </span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">گروه آلزایمر نشان داد و اختلاف معنی&shy;دار بود (001/0</span></span>P<<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">).</span></span><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:.1pt"></span></span></span></span></b></span></span></span></span><br> <span style="font-size:8pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt">بحث و نتیجه‌گیری</span></span></span></span></b><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">: </span></span><i><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">با توجه به نتایج مطالعه ممکن است بتوان گفت که</span></span></i><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> ترکیبات خارج سلولی حاصله از <i>لاکتوباسیلوس فرمنتوم</i> به علت داشتن اثرات آنتی اکسیدانی و ضد التهابی مانع پیشرفت ضایعات نورونی شده و در بهبود آلزایمر موثر باشد.</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""></span></span></span></span></span></span><br> &nbsp;<br> <br> محبوبه نخعی مقدم: <a href="https://scholar.google.com/citations?hl=en&user=IQ8PDHkAAAAJ">Google Scholar</a> <span style="font-size:9pt"><span new="" roman="" style="font-family:" times=""><b><span style="color:black"><span style="letter-spacing:-.2pt">Introduction:</span></span></b> Alzheimer&#39;s disease is a common progressive neurogenerative disease that leads to dementia and destruction of brain cells, especially in areas related to learning and memory such as the hippocampus. The role of the Arc gene in synaptic flexibility and memory consolidation has been proven and its expression is strongly influenced by neuronal activity. The aim of this study was to investigate the effect of <i>Lactobacillus fermentum</i> on the expression of Arc (effective in synaptic flexibility and memory consolidation) and CREB (effective in stabilizing synaptic changes during learning) genes, involved in the memory of Alzheimer&#39;s rats.</span></span><br> <span style="font-size:9pt"><span new="" roman="" style="font-family:" times=""><b><span style="color:black"><span style="letter-spacing:-.2pt">Material & ethods: </span></span></b>In this study that was conducted in 2019, 30 male Wistar rats were divided into 5 groups (control, Alzheimer and 3 groups as treatment). In the treatment groups, simultaneously with induction of Alzheimer by stereotaxic method with streptozotocin, supernatant of <i>Lactobacillus fermentum</i><b> </b>PTCC 1744<b> </b>in MRS broth<b> </b>with doses of 10⁸, 10⁷, and 10⁶ cfu/ml was injected intraperitoneally for 21 days. After RNA extraction from hippocampus samples, cDNA was synthesized and the expression of the genes was evaluated by real-time PCR and LinReg PCR software.<span style="font-size:12.0pt"><span style="color:#374151"></span></span></span></span><br> <span style="font-size:9pt"><span new="" roman="" style="font-family:" times=""><b><span style="color:black"><span style="letter-spacing:-.2pt">Findings:</span></span></b> The data showed an increase in gene expression of Arc and CREB in the treatment groups with a dose of 10⁶ cfu/ml compared to the Alzheimer&#39;s group and the difference was significant (P <0.001).</span></span><br> <span style="font-size:9pt"><span new="" roman="" style="font-family:" times=""><b><span style="color:black"><span style="letter-spacing:-.2pt">Discussion & conclusion:</span></span></b> Extracellular compounds of <i>L. fermentum</i> may inhibit the progression of neuronal lesions due to their antioxidant and anti-inflammatory effects and may be effective in improving Alzheimer&#39;s.</span></span><br> &nbsp; آلزایمر,Arc ,CREB , لاکتوباسیلوس فرمنتوم Alzheimer, Arc, CREB, Lactobacillus fermentum 36 46 http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-1399-3&slc_lang=fa&sid=1 Elnaz Mogaddam الناز مقدم moghaddamelnaz7902@gmail.com 9600319475328460047062 9600319475328460047062 No Dept of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran گروه زیست شناسی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران Maryam Tehranipur مریم طهرانی پور maryam_tehranipour@mshdiau.ac.ir 9600319475328460047063 9600319475328460047063 No Dept of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran گروه زیست شناسی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران Mahboobeh Nakhaei Moghaddam محبوبه نخعی مقدم mahboobe_nak@yahoo.com 9600319475328460047064 9600319475328460047064 Yes Dept of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran گروه زیست شناسی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران Khadijeh Nejad Shahrokhabadi خدیجه نژاد شاهرخ آبادی shahrokhabady@yahoo.com 9600319475328460047065 9600319475328460047065 No Dept of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran گروه زیست شناسی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران