fa مطالعۀ همراهی چندشکلی‌های ژن MTHFR در استعداد ابتلا به سرطان پستان ژنتیک Molecular Genetics پژوهشي Research <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:.1pt">مقدمه:</span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">سرطان پستان شایع‌ترین بدخیمی در زنان در سراسر جهان است؛</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> همچنین این سرطان دومین علت اصلی مرگ ناشی از ابتلا به سرطان در میان زنان، پس از سرطان ریه است. با توجه به ارتباط میان سطح فولات پلاسما، میزان یوراسیل و تخریب </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">DNA</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> در تقسیم سلولی، متیل تتراهیدروفولات ردوکتاز (</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">MTHFR</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">) کاندید مناسبی برای مطالعات استعداد ابتلا به سرطان ازجمله سرطان پستان است؛ بنابراین، هدف از این مطالعه بررسی همراهی میان دو چندشکلی ژن </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">MTHFR</span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> با خطر ابتلا به سرطان پستان در جمعیت زنان مبتلا در استان مرکزی است.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:.1pt">مواد و روش ها:</span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">در مطالعۀ حاضر، چندشکلی‌های </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">rs1801131</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">rs1801133</span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> ژن </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">MTHFR</span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> در جمعیت بزرگی مبتنی بر مطالعۀ مورد-شاهدی، شامل ۸۰ بیمار مبتلا به سرطان پستان و ۸۰ فرد سالم از زنان ساکن استان مرکزی و با فن </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">PCR-RFLP</span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> ارزیابی شدند و نمونه&shy;ها از طریق الکتروفورز ژل آگارز تعیین ژنوتایپ گردیدند. درنهایت، از نرم‌افزار </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">SPSS vol.16</span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> برای بررسی‌های آماری استفاده و نتایج نهایی مشخص شد.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt">یافته‌ها:</span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">از نظر آماری، اختلاف معناداری میان دو گروه بیمار و کنترل برای سه ژنوتیپ جایگاه </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">rs1801133</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> (</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">P=0.006</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">) مشاهده گردید. ژنوتیپ‌ </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">CT</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> (</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">P=0.001, OR=2.471, CI= 95%; 1.229 - 4.965</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">) و مجموع ژنوتیپ‌های </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">TT</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">&nbsp;CC</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">(</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">P=0.000, OR=3.375, CI=95%; 1.716 -6.637</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">) ارتباط معناداری با خطر ابتلا به سرطان پستان از خود نشان دادند، درحالی‌که ژنوتیپ </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">TT</span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> (</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">P=0.538</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">) نقشی حفاظتی از خود در برابر ابتلا به سرطان پستان نشان داد. در مقابل، از نظر آماری اختلاف معناداری میان دو گروه بیمار و کنترل برای سه ژنوتیپ جایگاه (</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">P=0.149</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">) </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">rs1801131</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> مشاهده نشد که این امر با برخی از مطالعات محققان مطابقت دارد.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt">بحث و نتیجه‌گیری:</span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">چندشکلی </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">rs1801133</span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> ژن </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">MTHFR</span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> می‌تواند به‌عنوان نشانگر در مطالعات پیش‌گویی بالینی در ارتباط با خطر ابتلا به سرطان پستان استفاده گردد.</span></span></span></span></span></span></span> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Introduction:</span></span></span></span></b> <span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Breast cancer is the most common malignancy in women worldwide. It is also the second leading cause of cancer death among women after lung cancer. Considering the relationship among plasma folate levels, the level of uracil, and DNA damage in cell division, methyl tetrahydrofolate reductase (MTHFR) is a suitable candidate for studies on the susceptibility to cancer, including breast cancer. This study aimed to investigate the relationship between two polymorphisms in the MTHFR gene and the risk of breast cancer among women in Markazi Province, Iran.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Material & Methods:</span></span></span></span></b> <span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">This case-control study investigated the rs1801131 and rs1801133 polymorphisms of the MTHFR gene and breast cancer risk in a large population including 80 patients and 80 healthy women in Markazi Province, Iran, using the PCR-RFLP technique. The specimens were genotyped by agarose gel electrophoresis. Finally, the data were analyzed in SPSS software (version 26).</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">(Ethic code<span style="text-transform:uppercase">: Ir.arakmu.rec.1396.25</span>)</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Findings:</span></span></span></span></b> <span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">There was a statistically significant difference between the two groups of patients and controls regarding three genotypes of the rs1801133 locus (P=0.006). Moreover, the CT genotype (P=0.001, OR=2.471, 95% CI=1.229-4.965) and total TT and CC genotypes (P=0.000, OR=3.375, 95% CI=1.716-6.637) were significantly associated with the risk of breast cancer. However, the TT genotype (P=0.538) showed a protective role against breast cancer. In contrast, there was no statistically significant difference between the two groups of patients and controls in terms of three genotypes of the rs1801131 locus (P=0.149), which is consistent with the results of some studies.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Discussion & </span></span></span></span></b><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">Conclusion:</span></span></span></b> <span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">The rs1801133 polymorphism of the MTHFR gene can be used as a marker in clinical prognostic studies related to the risk of breast cancer.</span></span></span></span></span><br> &nbsp; پلی‌مورفیسم, سرطان پستان, MTHFR, PCR-RFLP, rs1801131 و rs1801133 Breast cancer, MTHFR, PCR-RFLP, Polymorphism, rs1801131, rs1801133 21 31 http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-7015-2&slc_lang=fa&sid=1 Ahmad Hamta احمد همتا a-hamta@araku.ac.ir 9600319475328460046199 9600319475328460046199 Yes Dept of biology, Faculty of science, Arak University, Arak, Iran گروه زیست‌شناسی، دانشکدۀ علوم پایه، دانشگاه اراک، اراک، ایران Zahra Bozorgi Moghadam زهرا بزرگی مقدم mostafaemani22870@gmail.com 9600319475328460046200 9600319475328460046200 No Dept of biology, Faculty of science, Arak University, Arak, Iran گروه زیست‌شناسی، دانشکدۀ علوم پایه، دانشگاه اراک، اراک، ایران