Journal of Ilam University of Medical Sciences
مجله دانشگاه علوم پزشکی ایلام
J. Ilam Uni. Med. Sci.
Medical Sciences
http://sjimu.medilam.ac.ir
96
journal96
1563-4728
2588-3135
doi
fa
jalali
1401
9
1
gregorian
2022
12
1
30
5
online
1
fulltext
fa
مقایسۀ بیان مارکرهای CD44 و E-cadherin در لیکن پلان دهانی، لکوپلاکیای دهانی و سرطان سلولهای سنگفرشی دهان
Comparison of E- cadherin and CD44 Marker Expression in Oral Lichen Planus, Oral Leukoplakia, and Oral Squamous Cell Carcinoma
آسیب شناسی بالینی
Clinical pathology
پژوهشي
Research
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><span lang="AR-SA" style="font-size:14.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">مقدمه</span></span></b><b><span lang="AR-SA" style="font-size:9.0pt"><span style="background:white"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:.1pt">:</span></span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">نقش </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">E-cadherin</span></span><span style="font-size:9.0pt"><span style="font-family:"B Zar""> و </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">CD44</span></span><span style="font-size:9.0pt"><span style="font-family:"B Zar""> در توسعه و پیشرفت ضایعات سرطانی و پیشسرطانی ثابتشده است. بهنظر میرسد که این دو مارکر توانایی بالایی در ارزیابی پیشبدخیمی ضایعات لیکن پلان و لکوپلاکیای دهانی داشته باشند؛ بنابراین، مطالعۀ حاضر با هدف ارزیابی و مقایسۀ بیان دو مارکر </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">E-cadherin</span></span><span style="font-size:9.0pt"><span style="font-family:"B Zar""> و </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">CD44</span></span><span style="font-size:9.0pt"><span style="font-family:"B Zar""> در ضایعات لیکن پلان دهانی و لکوپلاکیای دهانی و اسکواموس سل کارسینومای دهانی انجام شد.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:.1pt">مواد و روش ها:</span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">این مطالعه از نوع توصیفی-تحلیلی است که روی 60 بلوک لیکن پلان، لکوپلاکیا و اسکواموس سل کارسینوما صورت گرفت. بلوکها توسط آنتیبادی </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">CD44</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> و </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">E-cadherin</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> رنگآمیزی شدند. دادههای بهدستآمده از این مطالعه با نرم</span></span><span dir="LTR" style="font-size:8.0pt"></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">افزار </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">SPSS vol.22</span></span><span style="font-size:9.0pt"><span style="font-family:"B Zar""> ارزیابی گردیدند و سطح معنیداری 05/0 در نظر گرفته شد.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">یافتهها:</span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">تنها 30 درصد از نمونههای </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">SCC</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> مارکر </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">CD44</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> را بیان کردند، درحالیکه در لکوپلاکیا و لیکن پلان، بهترتیب همۀ نمونهها 40 درصد و 50 درصد مارکر </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">CD44</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> را بروز دادند. میزان بیان مارکر </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">E-cadherin</span></span><span style="font-size:9.0pt"><span style="font-family:"B Zar""> در نمونههای </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">SCC</span></span><span style="font-size:9.0pt"><span style="font-family:"B Zar""> در 40 درصد موارد در وسعت رنگپذیری نشان داد، درحالیکه در لکوپلاکیا و لیکن پلان بهترتیب 60 درصد و 50 درصد بود. میان شدتپذیری رنگآمیزی </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">CD44</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> و </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">E-cadherin</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> هم تفاوت معناداری نشان داده نشد (</span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">P<0.16</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">)، درحالیکه در </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">SCC</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">، 70 درصد موارد شدت بیان ضعیف تا متوسط بود که از نظر آماری، در مقایسه با لیکن پلان و لکوپلاکیا معنادار است (</span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">P<0.004</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">).</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">بحث و نتیجهگیری:</span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">بر اساس یافتههای بهدستآمده از این تحقیق، بهنظر میرسد میزان شدت بروز </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">CD44</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> و </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">E-cadherin</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> میتواند بیانگر تغییرات دیسپلازی و پیشبدخیمی لکوپلاکیا و لیکن پلان دهانی در مقایسه با کارسینومهای دهانی باشد.</span></span></span></span></span></span></span><br>
<br>
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span dir="LTR" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif""></span></span></span></b></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:200%"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:16.0pt"><span style="line-height:200%"></span></span></b></span></span><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><b><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">Introduction:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="letter-spacing:-.2pt">The role of E-cadherin and CD44 has been proven in the development and progression of cancerous and precancerous lesions. It seems that these two markers have a high ability to evaluate the premalignancy of lichen planus lesions and oral leukoplakia. This study aimed to investigate and compare the expression of CD44 and E-cadherin markers in oral leukoplakia, lichen planus, and oral squamous cell carcinoma (SCC).</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><b><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">Material & Methods:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="letter-spacing:-.2pt">This analytical-descriptive study was conducted on 60 blocks of lichen planus, leukoplakia, and squamous cell carcinoma. The blocks were stained by CD44 and E-cadherin antibodies. The obtained data were analyzed in SPSS software (version 22), and a P-value of 0.05 was considered statistically significant.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><b><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">Findings:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="letter-spacing:-.2pt">Only 30% of the SCC samples expressed the CD44 marker, while 40% and 50% of leukoplakia and lichen planus samples expressed the CD44 marker, respectively. The expression of the E-cadherin marker in SCC samples was 40% in the range of staining, while it was 50% and 60% in leukoplakia and lichen planus, respectively. There was not a significant difference between the staining intensity of CD44 and E-cadherin (P<0.16). However, in SCC, 70% of the cases showed mild to moderate expression intensity that was statistically significant, compared to lichen planus and leukoplakia (P<0.004).</span></span></span></span></span></span><br>
<b><span style="font-size:9.0pt"><span style="line-height:97%"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">Discussion & </span></span></span></span></span></b><span style="font-size:10.0pt"><span style="line-height:97%"><span style="font-family:"Times New Roman",serif"><span style="letter-spacing:-.2pt">Conclusion: It seems that the severity of CD44 and E-cadherin incidence can indicate the changes in dysplasia and premalignancy of oral lichen planus and leukoplakia, compared to oral carcinomas.</span></span></span></span><br>
SCC, لکوپلاکیا, لیکن پلان, CD44 و E-cadherin
CD44, E-cadherin, Leukoplakia, Lichen planus, SCC
24
31
http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-877-2&slc_lang=fa&sid=1
Khadijeh
Abdal
خدیجه
ابدال
fariba4479@yahoo.com
9600319475328460045904
9600319475328460045904
No
Dept of Oral and Maxillofacial Pathology, Faculty of Dentistry, Ilam University of Medical Sciences, Ilam, Iran
گروه پاتولوژی دهان، فک و صورت، دانشکده دندانپزشکی، دانشگاه علوم پزشکی ایلام، ایلام، ایران
Samira
Mostafazede
سمیرا
مصطفی زاده
Dr.faribaabdal@yahoo.com
9600319475328460045906
9600319475328460045906
No
Dept of Oral and Maxillofacial Pathology, Faculty of Dentistry, Ilam University of Medical Sciences, Ilam, Iran
گروه پاتولوژی دهان، فک و صورت، دانشکده دندانپزشکی، دانشگاه علوم پزشکی ایلام، ایلام، ایران
Nastaran
Ghorbani
نسترن
قربانی
9600319475328460045907
9600319475328460045907
No
Dept of Oral and Maxillofacial Pathology, Faculty of Dentistry, Ilam University of Medical Sciences, Ilam, Iran
گروه پاتولوژی دهان، فک و صورت، دانشکده دندانپزشکی، دانشگاه علوم پزشکی ایلام، ایلام، ایران
Mohammadreza
Hafeziahmadi
محمدرضا
حافظی احمدی
reza.hafeziahmadi@gmail.com
9600319475328460045905
9600319475328460045905
Yes
Associate professor, Department of pathologogy, Faculty of medicine, Ilam University of Medical Sciences, Ilam, Iran.
گروه پاتولوژی عمومی، دانشکده پزشکی، دانشگاه علوم پزشکی ایلام، ایلام، ایران