en ارزیابی رابطۀ میان بیان گیرنده‌های فعال‌کنندۀ تکثیر پراکسی‌زوم (PPARα, PPARγ and PPARδ) و آنتی‌ژن کارسینوامبریونیک (CEA) در بیماران مبتلا به سرطان کولورکتال بیو شیمی بالینی clinical biochemistry پژوهشي Research <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">مقدمه</span></span></b><span lang="AR-SA" style="font-size:9.0pt"><span style="background:white"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">:</span></span></span></span> <span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">بر اساس مطالعات موجود، افزایش میزان آنتی‌ژن کارسینوامبریونیک (</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">CEA</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">) با پیشرفت سرطان کولورکتال همراه است و به‌عنوان یک عامل تشخیصی حساس برای </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">CRC</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> در نظر گرفته می‌شود و از سوی دیگر، اخیراً به نقش گیرنده‌های فعال‌کنندۀ تکثیر پراکسی&shy;زوم (</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">PPARs</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">) در سرطان کولورکتال توجه شده است. هدف از مطالعۀ حاضر بررسی رابطۀ میان میزان بیان </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">PPARs</span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> با </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">CEA</span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> در بیماران مبتلا به سرطان کولورکتال است.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">مواد و روش ها:</span></span></b> <span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">در این مطالعه، تعداد 100 نمونه بافت توموری اولیه به همراه نمونۀ بافت سالم مجاور آن و نیز نمونۀ سرمی بیماران مبتلا به سرطان کولورکتال که تحت عمل جراحی قرار گرفته بودند، از بانک تومور انستیتو کانسر تهران تهیه شد. میزان بیان </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">PPARs</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> در نمونه‌های بافتی و میزان </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">CEA</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> در نمونه‌های سرمی بیماران و نیز ارتباط میان این مارکرها ارزیابی گردید.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">یافته‌ها:</span></span></b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> نتایج به‌دست‌آمده از این مطالعه نشان داد که میزان بیان </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">PPARs</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> و نیز میزان سرمی </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">CEA</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> در سرطان کولورکتال به‌طور معنا‌داری افزایش می‌یابد (</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">P<0.01</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">). اگرچه میزان این مارکرها در بیماران با پیشرفت و گسترش بیماری ارتباط معنی‌داری نشان داد (</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">P<0.01</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">)؛ اما ارتباط معنا‌داری میان میزان بیان </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">PPARs</span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> و میزان سرمی </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">CEA</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">در این بیماران دیده نشد (</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">P>0.05</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">).</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">بحث و نتیجه‌گیری:</span></span></span></b> <span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">مطالعۀ کنونی نشان داد که میزان بیان </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">PPARs</span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> و نیز میزان سرمی</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">CEA </span></span>&nbsp;<span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">با پیشرفت و گسترش بیماری مرتبط است؛ اما ارتباط معنی‌داری میان میزان بیان </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">PPARs</span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> و میزان سرمی</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">CEA</span></span> <span style="font-size:9.0pt"><span b="" style="font-family:" zar="">&nbsp;در بیماران مبتلا به سرطان کولورکتال وجود ندارد.</span></span></span></span></span></span></span><br> <span dir="LTR"></span><span dir="LTR"></span> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Introduction:</span></span></span></span></b> <span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Studies have shown that an increase in carcinoembryonic antigen (CEA) is associated with the progression of colorectal cancer and is considered a sensitive diagnostic factor for CRC. Moreover, the role of peroxisome proliferators (PPARs) has recently been considered in colorectal cancer. This study aimed to investigate the relationship between the expression level of PPARs and CEA level in patients with colorectal cancer.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Material & Methods:</span></span></span></span></b> <span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">In this study, a total of 100 samples of primary tumor tissue along with adjacent healthy tissue samples and serum samples of patients with colorectal cancer who underwent surgery were prepared from the tumor bank of Tehran Cancer Institute in Thran, Iran. The expression level of peroxisome proliferator&nbsp;receptors (PRARs) in tissue samples and CEA level in serum samples were measured, and the relationship between these markers was evaluated as well.</span></span> <span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">The financial support for the study was provided by the Islamic Azad University (grant number 132655).</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">(</span></span><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Ethic code: </span></span><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">132655</span></span><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">)</span></span><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Findings:</span></span></span></span></b> <span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">The results of this study showed a significant increase in the expression level of PPARs and serum CEA levels in patients with colorectal cancer </span></span><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">(P<0.01)</span></span><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">. Although the level of these markers had a significant relationship with the progression and spread of disease in patients (P<0.01), no significant relationship was observed between the expression of PPARs and serum CEA in these patients (P>0.05).</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Discussion & Conclusion:</span></span></span></span></b> <span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Based on the obtained results, the expression level of PPARs and the serum CEA level are associated with the progression and spread of the disease; however, there is no significant relationship between the expression level of PPARs and the serum CEA level in patients with colorectal cancer.</span></span><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br> &nbsp; آنتی‌ژن کارسینوامبریونیک (CEA), سرطان کولورکتال,گیرنده‌های فعال‌کنندۀ تکثیر پراکسی‌زوم Carcinoembryonic antigen, Colorectal cancer, Peroxisome proliferator receptors 75 87 http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-6931-1&slc_lang=en&sid=1 Musa Yaghoubizadeh موسی یعقوبی زاده musa954261@gmail.com 9600319475328460045493 9600319475328460045493 No Dept of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran گروه زیست‌شناسی،واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران Leila Pishkar لیلا پیشکار leilapishkar@gmail.com 9600319475328460045494 9600319475328460045494 No Dept of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran گروه زیست‌شناسی، واحد اسلامشهر، دانشگاه آزاد اسلامی،اسلامشهر، ایران Gholam Basati غلام بساطی gholam.basati@gmail.com 9600319475328460045495 9600319475328460045495 Yes Dept of Clinical Biochemistry, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran گروه بیوشیمی بالینی، دانشکدۀ پزشکی، دانشگاه علوم پزشکی ایلام، ایلام، ایران