Journal of Ilam University of Medical Sciences
مجله دانشگاه علوم پزشکی ایلام
J. Ilam Uni. Med. Sci.
Medical Sciences
http://sjimu.medilam.ac.ir
96
journal96
1563-4728
2588-3135
doi
fa
jalali
1401
3
1
gregorian
2022
6
1
30
2
online
1
fulltext
fa
بررسی ارتباط میان پلیمورفیسم rs8192688 ژن FABP4 با استعداد ابتلا به بیماریهای قلبی- عروقی در بیماران دیابتی نوع 2 در استان ایلام
Investigation of the Relationship between the rs8192688 Polymorphism of the FABP-4 Gene and Cardiovascular Disease Susceptibility in Type 2 Diabetic Patients in Ilam Province, Iran
بیوشیمی
biochemistry
كاربردي
Applicable
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">مقدمه</span></span></b><span lang="AR-SA" style="font-size:9.0pt"><span style="background:white"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:.1pt">: </span></span></span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">بیماریهای قلبی-عروقی شایعترین علت مرگومیر در افراد مبتلا به </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">T2DM</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">است. ارتباط میان دیابت و بیماریهای قلبی و عروقی پیچیده و چندوجهی است. هدف از این مطالعه بررسی ارتباط میان پلیمورفیسم </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">rs8192688</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">ژن </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">FABP4</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">با استعداد ابتلا به بیماریهای قلبی- عروقی در بیماران دیابتی نوع 2 در استان ایلام بود.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black">مواد و روش ها:</span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">جامعۀ پژوهش در این تحقیق شامل 210 نفر (70 نفر بیمار دیابتی نوع 2 مبتلا به بیماری قلبی و عروقی، 70 نفر بیمار دیابتی نوع 2 بدون بیماری قلبی و عروقی و 70 نفر افراد سالم) بودند. </span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">فراوانی پلیمورفیسم بر اساس روش </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">Tetra</span></span><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif">-</span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">Primer</span></span><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif">-</span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">ARMS</span></span><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif">-</span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">PCR</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">انجام گرفت.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">یافتهها:</span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">نتایج نشان داد </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">TG</span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black">، </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">HDL</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">-</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">C</span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black">و </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">LDL-C</span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black">در گروه </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">T2DM with CVD</span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black">نسبت به گروه </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">T2DM</span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black">، بهطور معنیداری افزایش یافته بود (</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">P<0.05</span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black">). </span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">فراوانی ژنوتیپ </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">CT</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">در گروههای کنترل، دیابتی و دیابتی به همراه بیماریهای قلبی-عروقی بهترتیب 26، 24 و 29 درصد (</span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">P=0.16</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">)، فراوانی ژنوتیپ </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">TT</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">بهترتیب 6، 4 و 4 درصد (</span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">P=0.12</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">) و فراوانی آلل </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">T</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">بهترتیب 19، 16 و 19 درصد (</span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">P=0.09</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">) بود. ارتباط معناداری میان این پلیمورفیسم و گروههای مطالعهشده وجود نداشت.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">بحث و نتیجهگیری: </span></span></span></span></b><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">نتایج نشان داد، </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">TG</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">، </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">LDL-C</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> و </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">HDL-C</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> میتوانند در توسعۀ </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">CVD</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">در بیماران </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">T2DM</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">نقش داشته باشند؛ اما پلیمورفیسم </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">rs8192688</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">ژن </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">FABP4</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">در توسعۀ </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">CVD</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">در بیماران </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">T2DM</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">نقش ندارد.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><b><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.1pt">Introduction:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif">Cardiovascular disease (CVD) is the most common cause of death in patients with type 2 diabetes (T2D). The association between diabetes and cardiovascular disease is complex and multifaceted. This study aimed to investigate the possible association between rs8192688 Polymorphism of the <i>FABP-4</i> gene and the CVD susceptibility in T2DM patients in Ilam Province, Iran.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><b><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:.1pt">Material & Methods:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif">The statistical population consisted of 210 cases (T2D patients with CVD [n=70], T2D patients without CVD [n=70], and healthy controls [n=70]). The frequency of polymorphisms was investigated using the Tetra-Primer-ARMS-PCR method.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif">(Ethic code: IR.IAU.SHK.1399.011)</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><b><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.1pt">Findings:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif">The results showed that TG, HDL-C, and LDL-C in the T2D group with CVD were significantly increased, compared to those in the T2D group (P<0.05). The frequency rates of the CT genotype in the control, T2D, and T2D with CVD groups were 26%, 24%, and 295 (P=0.16), respectively. Moreover, the frequency rates of the TT genotype were 6%, 4%, and 4% (P=0.12), and the frequency rates of the T allele were 19%, 16%, and 19% (P=0.09), respectively. There was no significant relationship between this polymorphism and the studied groups.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><b><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.1pt">Discussion & Conclusion:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif">The results showed that TG, LDL-C, and HDL-C could have a role in the development of CVD in T2D patients; however, the rs8192688 polymorphism of the <i>FABP4</i> gene was not involved in the development of CVD in T2D patients.</span></span></span></span></span><br>
<span dir="RTL"></span>
FABP-4, بیماریهای قلبی-عروقی, پلیمورفیسم, دیابت نوع 2
Cardiovascular disease, FABP-4, Polymorphism, Type 2 diabetes
97
104
http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-6803-1&slc_lang=fa&sid=1
Amin
Bakhtiyari
امین
بختیاری
Aminbkht@gmail.com
9600319475328460045299
9600319475328460045299
No
Dept of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
گروه زیستشناسی، دانشکدۀ علوم پایه، دانشگاه آزاد اسلامی، واحد شهرکرد، شهرکرد، ایران
Salar
Bakhtiari
سالار
بختیاری
bakhtiyaribio@gmail.com
9600319475328460045300
9600319475328460045300
Yes
Dept of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
گروه زیستشناسی، دانشکدۀ علوم پایه، دانشگاه آزاد اسلامی، واحد شهرکرد، شهرکرد، ایران
Maryam
Peymani
مریم
پیمانی
peymani62_m@yahoo.com
9600319475328460045301
9600319475328460045301
No
Dept of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
گروه زیستشناسی، دانشکدۀ علوم پایه، دانشگاه آزاد اسلامی، واحد شهرکرد، شهرکرد، ایران
Karimeh
Haghani
کریمه
حقانی
haghani.bio@gmail.com
9600319475328460045302
9600319475328460045302
No
Dept of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
گروه زیستشناسی، دانشکدۀ علوم پایه، دانشگاه آزاد اسلامی، واحد شهرکرد، شهرکرد، ایران
Siros
Norozi
سیروس
نوروزی
drsirousnourozi@gmail.com
9600319475328460045303
9600319475328460045303
No
Dept of Cardiology, Ilam University of Medical Sciences, Ilam, Iran
گروه قلب و عروق، دانشکدۀ پزشکی، دانشگاه علوم پزشکی ایلام، ایلام، ایران