fa بررسی آثار ضدمیکروبی عصارۀ آبی_الکلی گیاه آرتمیزیا تورانیکا (Artemisia Turanica) علیه استافیلوکوکوس اورئوس مقاوم به متی‌سیلین (MRSA): یک مطالعۀ آزمایشگاهی منابع طبیعی Natural Resources پژوهشي Research <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">مقدمه</span></span></b><span lang="AR-SA" style="font-size:9.0pt"><span style="background:white"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">:</span></span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">در بسیاری از کشورهای جهان، عفونت‌های ایجادشده به‌وسیلۀ استافیلوکوکوس اورئوس مقاوم به متی‌سیلین (</span></span><span dir="LTR" style="font-size:7.0pt"><span new="" roman="" style="font-family:" times="">MRSA</span></span><span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">) در حال افزایش است و پژوهشگران به دنبال یافتن ترکیبات جدید با منشأ طبیعی به‌عنوان جایگزین آنتی‌بیوتیک‌ها در درمان این عفونت‌ها هستند؛ بنابراین، مطالعۀ</span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">حاضر</span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">با</span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">هدف</span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">بررسی</span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">آثار</span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">ضدمیکروبی عصارۀ</span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">گیاه</span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">بومی ایران، آرتمیزیا تورانیکا</span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">انجام</span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">گرفت.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">مواد و روش ها:</span></span></b> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">در</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">این</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">تحقیق</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">پس از عصاره‌گیری گیاه،</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">بررسی</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">اثر</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">ضدباکتریایی</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">عصارۀ</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">آبی-الکلی</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">گیاهان</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">علیه</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">سویۀ مقاوم به دارو، به</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">روش</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">دیسک دیفیوژن</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">انجام</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">شد.</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">هر</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">آزمون</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">سه</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">بار</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">تکرار</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">گردید</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">و میانگین</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">قطر</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">هالۀ</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">عدم</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">رشد</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">اندازه‌گیری</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">و</span></span></span> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">ثبت شد. در پایان، با روش میکروبراث دایلوشن، کمینۀ غلظت بازدارندگی رشد (</span></span></span><span dir="LTR" style="font-size:7.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">MIC</span></span></span><span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">) و کمینۀ غلظت کشندگی (</span></span></span><span dir="LTR" style="font-size:7.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">MBC</span></span></span><span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">) عصارۀ گیاه علیه </span></span></span><span dir="LTR" style="font-size:7.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">MRSA</span></span></span><span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt"> تعیین گردید.</span></span></span><span lang="FA" style="font-size:8.0pt"><span style="font-family:"B Zar""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">یافته‌ها: </span></span></b><span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">نتایج نشان داد فعالیت ضدمیکروبی عصارۀ گیاه (5/1</span></span><span lang="FA" style="font-size:8.0pt"><span cambria="" style="font-family:">&plusmn;</span></span><span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">29) نسبت به آنتی‌بیوتیک ونکومایسین (4/1</span></span><span lang="FA" style="font-size:8.0pt"><span cambria="" style="font-family:">&plusmn;</span></span><span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">14)، علیه استافیلوکوکوس‌ اورئوس مقاوم به متی‌سیلین (</span></span><span dir="LTR" style="font-size:7.0pt"><span new="" roman="" style="font-family:" times="">MRSA</span></span><span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">) بیشتر بود. نتایج همچنین نشان داد اثر کمینۀ غلظت مهارکنندگی بر </span></span><span dir="LTR" style="font-size:7.0pt"><span new="" roman="" style="font-family:" times="">MRSA</span></span><span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar=""> برابر 98 میکروگرم بر میلی‌لیتر و کمینۀ غلظت کشندگی آن 157 میکروگرم بر میلی‌لیتر است. عصارۀ گیاه فعالیت ضدقارچی مناسبی در برابر سویه‌های قارچی بیماری‌زا نشان داد.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">بحث و نتیجه‌گیری:</span></span></span></b> <span lang="FA" style="font-size:8.0pt"><span b="" style="font-family:" zar="">با توجه به فعالیت ضد‌‌میکروبی قوی عصارۀ گیاه علیه استافیلوکوکوس اورئوس مقاوم به متی‌‌سیلین با تولید، خالص‌سازی و شناسایی ترکیبات فعال این عصارۀ در مقیاس صنعتی، امکان معرفی ترکیبات ضدمیکروبی جدید مطرح می‌شود.</span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Introduction:</span></span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are on the rise worldwide. Over the last few decades, new compounds of natural origins have regained interest as an alternative to antibiotics in the treatment of MRSA infections. This study aimed to investigate the antimicrobial effects of Artemisia Turanica extract, a native plant of Iran.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Material & Methods:</span></span></span></b> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">The plant was subjected to aqueous-alcoholic extraction, and its antibacterial effects were then evaluated using the disk diffusion method. All tests were performed in triplicate, and the mean diameter of the growth inhibition zone was recorded. Finally, the minimum inhibitory concentrations and minimum bactericidal concentrations (MIC and MBC) against MRSA were determined using the micro broth dilution method</span></span><span dir="RTL" lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="line-height:normal"><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">(Ethic code: </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">IR.MUMS.REC.1401.015</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">)</span></span><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Findings:</span></span></span></b> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">The recorded </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">antimicrobial activity against MRSA of the plant extract (29&plusmn;1.5) was higher than that of the vancomycin antibiotic (14&plusmn;1.4). The effect of MIC on MRSA was recorded at 98 &mu;g/mL, and the MBC was estimated at 157 </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">&mu;g/mL. The plant extract also showed antifungal activity against the tested pathogenic fungal strains</span></span><span dir="RTL" lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Discussion & Conclusion:</span></span></span></b> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Artemisia Turanica extract has a strong antibacterial activity against MRSA. Accordingly, the purification and identification of active compounds of this extract on an industrial scale pave the way for introducing new antimicrobial compounds.</span></span></span></span></span><br> &nbsp; اثر ضدباکتریایی, عصارۀ گیاه, مقاومت دارویی, MRSA Antibacterial effect, Drug resistance, MRSA, Plant extract 1 7 http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-6773-1&slc_lang=fa&sid=1 Hasan Bagheri yazdi حسن باقری یزدی bagheriyazdi@cfu.ac.ir 9600319475328460045222 9600319475328460045222 Yes Farhangian University ,Iran گروه علوم پایه، دانشگاه فرهنگیان، ایران Hamed Norouzi taheri حامد نوروزی طاهری hamed.nt68@yahoo.com 9600319475328460045223 9600319475328460045223 No Farhangian University, Mashhad, Iran. گروه علوم پایه، دانشگاه فرهنگیان، ایران