Journal of Ilam University of Medical Sciences
مجله دانشگاه علوم پزشکی ایلام
J. Ilam Uni. Med. Sci.
Medical Sciences
http://sjimu.medilam.ac.ir
96
journal96
1563-4728
2588-3135
doi
fa
jalali
1401
5
1
gregorian
2022
8
1
30
3
online
1
fulltext
fa
آثار اسنیک اسید بر آپوپتوز و بیان پروتئینهای Bax و Bcl-2 نورونهای ناحیۀ CA1 هیپوکامپ به دنبال ایسکمی- ریپرفیوژن مغزی
Effects of Usnic Acid on Apoptosis and Expression of Bax and Bcl-2 Proteins in Hippocampal CA1 Neurons Following Cerebral Ischemia-Reperfusion
فیزیولوژی
physiology
پژوهشي
Research
<span style="font-size:11pt"><span style="tab-stops:319.65pt right 322.35pt"><span style="line-height:97%"><span calibri="" style="font-family:"><b> </b><b><span dir="RTL" lang="FA" style="font-size:10.0pt"><span style="line-height:97%"><span b="" nazanin="" style="font-family:"><span style="color:#1c57ce"><span style="position:relative"><span style="top:-1.0pt"></span></span></span></span></span></span></b></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">مقدمه</span></span></b><span lang="AR-SA" style="font-size:9.0pt"><span style="background:white"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">:</span></span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">ایسکمی- ریپرفیوژن مغزی باعث ایجاد سازوکارهای پیچیدۀ آسیب بافتی ازجمله آپوپتوز نورونها میشود. اسنیک اسید یک متابولیت ثانویه از گلسنگ است که خواص بیولوژیکی مختلفی چون فعالیتهای آنتیاکسیدانی و ضدالتهابی دارد. هدف از مطالعۀ حاضر بررسی آثار محافظتکنندۀ عصبی اسنیک اسید بر مرگ سلولی آپوپتوز و پروتئین های مرتبط با آپوپتوز </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">Bax</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">و </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">Bcl</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">-</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">2</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">در نورونهای ناحیۀ </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">CA1</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">هیپوکامپ به دنبال ایسکمی- ریپرفیوژن مغزی بود.</span></span><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">مواد و روش ها:</span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">42 موش صحرایی نر نژاد ویستار بهطور تصادفی به سه گروه (شم، ایسکمی- ریپرفیوژن و ایسکمی- ریپرفیوژن + اسنیک اسید) تقسیم شدند. ایسکمی مغزی با انسداد شریانهای کاروتید مشترک ایجاد گردید. تزریق اسنیک اسید (</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">mg/kg</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">25، بهصورت داخل صفاقی) در آغاز برقراری دوبارۀ جریان خون صورت گرفت. میزان بیان پروتئین های </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">Bax</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">و </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">Bcl</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">-</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">2</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">با استفاده از روش ایمونوفلورسنت اندازهگیری شد. برای تعیین میزان مرگ سلولی آپوپتوزی از رنگآمیزی </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">TUNEL</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">استفاده گردید</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">یافتهها:</span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">ایسکمی مغزی موجب افزایش آپوپتوز نورونهای ناحیۀ </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">CA1</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">هیپوکامپ شد. اسید اسنیک از طریق کاهش بیان پروتئین پیش آپوپتوزی </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">Bax</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">و افزایش بیان پروتئین آنتیآپوپتوزی </span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">Bcl</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">-</span></span><span dir="LTR" style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">2</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، میزان مرگ سلولی آپوپتوزی را به شکل چشمگیری کاهش داد.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">بحث و نتیجهگیری:</span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">اسنیک</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">اسید از طریق کاهش</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">مرگ سلولی میتواند اثر</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">محافظتی</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">عصبی</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">در</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">برابر</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">آسیب</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""></span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">های ناشی</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">از</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">ایسکمی- ریپرفیوژن مغزی</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">داشته</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">باشد.</span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="text-autospace:none"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Introduction:</span></span></span></span></b> <span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Cerebral ischemia-reperfusion causes complex pathological mechanisms that lead to tissue damage, such as neuronal apoptosis. Usnic acid is a secondary metabolite of lichen and has various biological properties including antioxidant and anti-inflammatory activities. This study aimed to investigate the neuroprotective effects of usnic acid on apoptotic cell death and apoptotic-related proteins (Bax and Bcl-2) in the CA1 area of the hippocampus following cerebral ischemia-reperfusion.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="text-autospace:none"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Material & Methods:</span></span></span></span></b> <span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">A total of 42 male Wistar rats were randomly divided into three groups (sham, ischemia-reperfusion, and ischemia-reperfusion+usnic acid). Ischemia was induced by occlusion of both common carotid arteries for 20 min. Usnic acid (25 mg/kg, intraperitoneally) was injected at the beginning of reperfusion time. The expression levels of Bax and Bcl-2 proteins were measured using the immunofluorescence method, and the rate of apoptotic cell death was determined using the TUNEL staining method.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">(</span></span><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Ethic code: IR.SHMU.REC.1397.186)</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="text-autospace:none"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Findings:</span></span></span></span></b> <span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">In this study, cerebral ischemia increased apoptotic cell death in the CA1 area of the hippocampus. It was demonstrated that usnic acid significantly decreased apoptotic cell death by decreasing the expression of Bax pro-apoptotic protein and increasing the expression of Bcl-2 (anti-apoptotic protein).</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="text-autospace:none"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Discussion & Conclusion:</span></span></span></span></b> <span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">It can be concluded that usnic acid has a neuroprotective effect against cerebral ischemia-reperfusion damages by reducing cell death.</span></span></span></span></span></span></span></span><br>
آپوپتوز, اسنیک اسید, ایسکمی- ریپرفیوژن, هیپوکامپ
Apoptosis, Hippocampus, Ischemia-reperfusion, Usnic acid
101
111
http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-6762-1&slc_lang=fa&sid=1
Sohaila
Erfani
سهیلا
عرفانی
sohailaerfani@gmail.com
9600319475328460045534
9600319475328460045534
No
Dept of Biology, Faculty of Science, Ilam University, Ilam, Iran
گروه زیستشناسی، دانشکدۀ علوم پایه، دانشگاه ایلام، ایلام، ایران
Tahereh
Valadbeigi
طاهره
ولدبیگی
tvaladbeigi@yahoo.com
9600319475328460045535
9600319475328460045535
No
Dept of Biology, Faculty of Science, Ilam University, Ilam, Iran
گروه زیستشناسی، دانشکدۀ علوم پایه، دانشگاه ایلام، ایلام، ایران
Mehdi
Khaksari
مهدی
خاکساری
khaksari417@yahoo.com
9600319475328460045536
9600319475328460045536
No
Faculty of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
گروه فیزیولوژی پزشکی، دانشکدۀ پزشکی، دانشگاه علوم پزشکی شاهرود، شاهرود، ایران
Ali
Moghimi
علی
مقیمی
moghimi@um.ac.ir
9600319475328460045537
9600319475328460045537
No
Rayan Research Center for Neuroscience and Behavior, Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
مرکز پژوهشی علوم اعصاب و رفتار رایان، گروه زیستشناسی، دانشکدۀ علوم، دانشگاه فردوسی مشهد، ایران
Nahid
Aboutaleb
ناهید
ابوطالب
dr.nabotaleb@gmail.com
9600319475328460045538
9600319475328460045538
Yes
Physiology Research Center, Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
مرکز تحقیقات فیزیولوژی و گروه فیزیولوژی، دانشکدۀ پزشکی، دانشگاه علوم پزشکی ایران، تهران، ایران