Journal of Ilam University of Medical Sciences
مجله دانشگاه علوم پزشکی ایلام
J. Ilam Uni. Med. Sci.
Medical Sciences
http://sjimu.medilam.ac.ir
96
journal96
1563-4728
2588-3135
doi
fa
jalali
1401
7
1
gregorian
2022
10
1
30
4
online
1
fulltext
fa
بررسی واریانتهای اگزون 1 ژن NOEY2 با سندروم تخمدان پلیکیستیک (PCOS) و دیابت
Association of Exon 1 Variants of NOEY2 Gene with Poly cystic Ovary Syndrome (PCOS) and Diabetes
ژنتیک
Molecular Genetics
پژوهشي
Research
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<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar"">مقدمه</span></span></b><b><span lang="AR-SA" style="font-size:9.0pt"><span style="background:white"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:.1pt">: </span></span></span></span></span></b><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">سندروم تخمدان پلیکیستیک </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">(Polycystic ovary syndrome: PCOS)</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> علت 75 درصد ناباروری</span></span><span dir="LTR" lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">های ناشی از نداشتن تخمکگذاری است. مطالعۀ حاضر با هدف بررسی ارتباط احتمالی میان پلی‏مورفیسمهای اگزون 1 ژن </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">NOEY2</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> در زنان مبتلا به </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">PCOS</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> و دیابت انجامشده است.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:.1pt">مواد و روش ها:</span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">در این مقاله، تعداد 240 نمونۀ خون زنان (هرکدام به میزان </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">cc</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">1) شامل چهار گروه پلیکیستیک، دیابت، پلیکیستیک-دیابت و کنترل مطالعه شدند. استخراج </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">DNA</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> ژنومیک افراد توسط کیت استخراج و بر اساس دستورالعمل صورت گرفت. یک جفت پرایمر برای توالی اگزون 1 ژن </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">NOEY2</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> طراحی و سنتز گردید. پس از انجام مراحل تکثیر ژن توسط واکنش زنجیرهای پلیمراز (</span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">PCR</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">) و بررسی کیفیت باندها، توالییابی سانگر روی محصولات </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">PCR</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> صورت گرفت. نتایج توالی</span></span><span dir="LTR" lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">یابی توسط نرم</span></span><span dir="LTR" lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">افزارهای </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">Chromas</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">، </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif"> GeneRunner</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> و </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">Alignment</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> و محاسبات آماری توسط نرمافزارهای </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">SNPAlyze</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">، </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">SPSS</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> و </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">Web-Assotest</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> بررسی و محاسبه شدند.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">یافتهها:</span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">تحلیل توالی</span></span><span dir="LTR" lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">ها وجود دو پلی</span></span><span dir="LTR" lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">مورفیسم را تأیید کرد: پلی</span></span><span dir="LTR" lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">مورفیسم </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">5’UTR c.156G>A</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> در ناحیۀ اگزون 1 ژن </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">NOEY2</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> و پلیمورفیسم دوم </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">5’UTR c.156G>A</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">، 76 نوکلئوتید دورتر از اگزون 1 و در ناحیۀ اینترون 1 ژن </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">.NOEY2 </span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> پس از بررسیهای آماری، اختلاف معنیداری از نظر آماری میان </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">5’UTR c.156G>A</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> و استعداد ابتلا به </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">PCOS</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> و دیابت مشاهده گردید.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">بحث و نتیجهگیری:</span></span></span></span></b> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">با توجه به نبودِ گزارش این ناحیه در پایگاههای دادۀ ژنتیکی، این پلیمورفیسم اولین بار در مطالعۀ حاضر گزارششده است و مطالعات بیوانفورماتیکی نشان داد که این پلیمورفیسم به</span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar"">سبب قرارگیری در ناحیۀ پروموتری همپوشان با </span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Times New Roman",serif">CTCF</span></span><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Zar""> میتواند تمایل اتصال عاملهای رونویسی خاصی را با تغییر آللی تحت تأثیر قرار دهد.</span></span></span></span></span></span></span><br>
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<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><b><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">Introduction:</span></span></span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">Polycystic ovary syndrome (PCOS) is the cause of 75% of infertility due to lack of ovulation. This study aimed to investigate the possible association between exon 1 polymorphisms of the NOEY2 gene in women with PCOS and diabetes.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><b><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">Material & Methods:</span></span></span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">This study was conducted on 240 blood samples (1 cc each) from women in four groups: polycystic, diabetes, polycystic-diabetes, and control. Genomic DNA extraction was performed using the extraction kit, according to the protocol. A primer pair was designed and synthesized for the exon 1 sequence of the NOEY2 gene. After gene amplification by polymerase chain reaction (PCR) and evaluation of band quality, Sanger sequencing was performed on PCR products. Sequencing results were analyzed using Chromas, GeneRunner, and Alignment software, and statistical calculations were performed by SNPAlyze, SPSS, and Web-Assotest program.</span></span><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><span style="font-size:9.5pt"><span style="font-family:"Times New Roman",serif">(Ethic code: </span></span><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">IR.IAU.TON.REC.137031</span></span><span style="font-size:9.5pt"><span style="font-family:"Times New Roman",serif">)</span></span><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><b><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">Findings:</span></span></span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">Sequence analysis confirmed two polymorphisms: 5'UTR c.156G> A polymorphism was observed in exon 1 of NOEY2 and second polymorphism of 5'UTR c.207+76 G>A, 76 nucleotides farther from exon 1 and in the intron region of NOEY2 gene. Statistical analysis showed a statistically significant difference between 5'UTR c.156G> A and susceptibility to PCOS and diabetes.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><b><span style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">Discussion & Conclusion:</span></span></span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">No report on this region was available in genetic databases and this polymorphism was reported for the first time in the present study. Bioinformatics studies showed that this polymorphism can affect the affinity to bind in certain transcription factors by allelic changes due to its location in the CTCF overlapping promoter region.</span></span></span></span></span><br>
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پلیمورفیسم, دیابت, سندروم تخمدان پلیکیستیک, ژن NOEY2
Diabetes, Polymorphism, NOEY2 gene, Polycystic ovary syndrome
106
116
http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-6666-1&slc_lang=fa&sid=1
Parichehr
Karajibani
پریچهر
کرجی بانی
parichehr.karajibani@gmail.com
9600319475328460045793
9600319475328460045793
No
Dept of Genetics, Faculty of Basic Science, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran
گروه ژنتیک، دانشکدۀ علوم زیستی، دانشگاه آزاد اسلامی، واحد تنکابن، تنکابن، ایران
Abolhasan
Rezaei
ابوالحسن
رضایی
a.rezaei7300@gmail.com
9600319475328460045792
9600319475328460045792
Yes
Dept of Genetics, Faculty of Basic Science, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran
گروه ژنتیک، دانشکدۀ علوم زیستی، دانشگاه آزاد اسلامی، واحد تنکابن، تنکابن، ایران