fa پیشگویی بیوانفورماتیکی ژن‌های غیرکدکنندۀ مرتبط با ژن‌های مسیر تمایز اکتودرمی FGF8، NOG و BMP4 موشی و ترسیم شبکۀ ارتباطی آن‌ها ژنتیک Molecular Genetics پژوهشي Research <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">مقدمه</span></span></b><span lang="AR-SA" style="font-size:9.0pt"><span style="background:white"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:.1pt">: </span></span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">lncRNA</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">ها و </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">miRNA</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">ها دسته‌ای از </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">RNA</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">های غیرکدکننده هستند که نقش و عملکردهای مهمی در تنظیم بیان ژن‌ها و فرایندهای اصلی بیولوژیکی ازجمله تکثیر سلولی، آپوپتوز و تمایز دارند. </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">lncRNA</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">ها می‌توانند به‌طور بالقوه روی </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">miRNA</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">ها، در جهت همسو و یا معکوس بر فعالیتشان تأثیر بگذارند تا از این طریق، نقش تنظیم‌کنندگی آن‌ها را تعدیل کنند. در این مطالعه، شبکه‌های ژنی </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">mRNA-miRNA-lncRNA</span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> با استفاده از برنامه‌های آنلاین برای سه مارکر مسیر اکتودرمی (</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">BMP4,NOG,FGF8</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">) در سلول‌های بنیادی جنینی موشی ترسیم شد.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:.1pt">مواد و روش ها:</span></span></span></span></b> <span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">این مطالعه از نوع تئوریکال بیوانفورماتیکی است و </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">micRNA</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">های هدف ژن‌های </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">BMP4</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">NOG</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">FGF8</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> توسط پایگاه‌های داده‌ای </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">MirWalk</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">TARGETSCAN</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> استخراج و بررسی گردید تا درنهایت، بتوان </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">miRNA</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">های مشترک این ۳ ژن را تهیه کرد؛ سپس از پایگاه داده‌ای </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">DIANA-Tools</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">lncRNA</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">های هدف برای </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">miRNA</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">های مشترک به‌دست آمد.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt">یافته‌ها:</span></span></span></span></b> <span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">ژن‌های </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">mmu-miR-92a-2-5p</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">mmu-miR-129b-5p</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">mmu-miR-130b-5p</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">mmu-miR-692</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">mmu-miR-7009-3P</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">mmu-miR-7116-3p</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">mmu-miR-7689-3p</span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> ممکن است بر فعالیت </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">lncRNA‌</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">های </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Kcnq1ot1</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Gm26812</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Gm4117</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Gm11837.4930423MO2Rik</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Malat1</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Gm12594</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Gm3414.5830444B04Rik</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Gm2464</span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">NEAT1</span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""> اثر بگذارند.</span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:.2pt">بحث و نتیجه‌گیری:</span></span></span></span></b> <span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.2pt">با توجه به ارتباط متقابل </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.2pt">lncRNA</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.2pt">، </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.2pt">miRNA</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.2pt"> و </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.2pt">mRNA</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.2pt">، مطالعۀ ما یک نقش جدید از </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.2pt">lncRNA</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.2pt">ها را برای تحقیقات آینده و مطالعات تجربی دربارۀ مسیر تمایز اکتودرمی و سازوکار‌های مولکولی فراهم می‌کند.</span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Introduction:</span></span></span></span></b> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Long non-coding RNAs (lncRNAs) and miRNAs belong to a class of non-coding RNAs (ncRNAs) that play important roles and functions in the regulation of the expression of genes in main biological processes, such as cell proliferation, apoptosis, and differentiation. LncRNAs can potentially affect miRNAs in the forms of cis/trans to modulate their regulatory role. In this study, mRNA, miRNA, and lncRNA gene networks were predicted by web-based programs for three ectodermal pathway markers (BMP4, NOG, FGF8) in the mouse embryonic stem cells.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:.1pt">Material & Methods:</span></span></span></span></b> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">In this theoretical bioinformatics study, the miRNAs of the target genes (BMP4, NOG, and FGF8) were extracted and examined by MirWalk and TARGETSCAN databases to finally obtain the common miRNAs of these three genes. Following that, the target lncRNAs for common miRNAs were then extracted from the DIANA-Tool database.</span></span><span dir="RTL" lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span><br> <span style="line-height:normal"><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">(Ethic </span></span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">code: 100/21560/2/</span></span></span><span dir="RTL" lang="FA" style="font-size:10.0pt"><span style="background:white"><span b="" nazanin="" style="font-family:">پ</span></span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">)</span></span></span> <span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Findings:</span></span></span></span></b> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MiRs mmu-miR-92a-2-5p, mmu-miR-129b-5p, mmu-miR-130b-5p, mmu-miR-692, mmu-miR-7009-3P, mmu-miR-7116-3p, and mmu-miR-7689-3p</span></span> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">may affect the function of lncRNAs, including Kcnq1ot1, Gm26812, Gm4117, Gm11837, 4930423MO2Rik, Malat1, Gm12594, Gm3414, 5830444B04Rik, Gm2464, and NEAT1.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:.1pt">Discussion & Conclusion:</span></span></span></span></b> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Due to the mutual relationships among lncRNA, miRNA, and mRNA, our results provided a novel perspective on lncRNAs for future research and experimental studies on ectodermal differentiation pathways and molecular mechanisms.</span></span></span></span></span><br> &nbsp; بیوانفورماتیک, سامانۀ بیولوژی, BMP4, FGF8 اکتودرم, RNAهای غیرکدکننده Bioinformatics, Biological system, BM4, Ectoderm, FGF8, Non-coding RNAs 29 41 http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-6631-2&slc_lang=fa&sid=1 Somayeh Moghaddam سمیه مقدم smyhmqdm@gmail.com 9600319475328460044940 9600319475328460044940 No Dept of Animal Biology, School of Natural Sciences, University of Tabriz, Tabriz, Iran گروه علوم جانوری، دانشکدۀ علوم طبیعی، دانشگاه تبریز، تبریز، ایران Esmaeil Babaei اسمائیل بابایی babaei@tabrizu.ac.ir 9600319475328460044941 9600319475328460044941 Yes Dept of Animal Biology, School of Natural Sciences, University of Tabriz, Tabriz, Iran گروه علوم جانوری، دانشکدۀ علوم طبیعی، دانشگاه تبریز، تبریز، ایران