fa تاثیر اسید آسکوربیک بر مسیرهای سیگنالینگ ژن های Bcl2 و Bax در رده‌ سلولی AGS ژنتیک Molecular Genetics پژوهشي Research <strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">مقدمه:</span></span></em></strong> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">آنتی &shy;اکسیدان ها تاثیر زیادی در مهار استرس اکسیداتیو و رادیکال های آزاد داشته و باعث بیان ژن های آپوپتوز می شوند. بنـــا بر این آنتی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">اکسیدان ها نقش مهمی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">در پیشگیری از سرطان دارند. هدف از این پژوهش تاثیر اسید آسکوربیک بر مسیرهای سیگنالینگ ژن های </span></span><span dir="LTR"><span style="font-size:10.0pt;">Bcl2</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">Bax</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> در رده سلولی </span></span><span dir="LTR"><span style="font-size:10.0pt;">AGS</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> می &shy;باشد.</span></span><br> <strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">مواد و روش &shy;ها:</span></span></em></strong> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">در این پژوهش رده سلولی </span></span><span dir="LTR"><span style="font-size:10.0pt;">AGS</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> با غلظت های مختلف اسید آسکوربیک در بازه &shy;های زمانی مختلف تیمار شدند. اثر</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">اسید آسکوربیک</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بر</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">میزان</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مرگ و میر</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">سلول</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ها</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">با</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">روش </span></span><span dir="LTR"><span style="font-size:10.0pt;">MTT</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> اندازه&shy; گیری شد. استخراج </span></span><span dir="LTR"><span style="font-size:10.0pt;">RNA</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> و سنتز </span></span><span dir="LTR"><span style="font-size:10.0pt;">cDNA</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> صورت گرفت. در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">نهایت،</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بیان</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ژن </span></span><span dir="LTR"><span style="font-size:10.0pt;">Bcl2</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">Bax</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> با</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">استفاده</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">روش </span></span><span dir="LTR"><span style="font-size:10.0pt;">Real Time PCR</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> مورد</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بررسی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">قرار</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">گرفت</span></span><span dir="LTR"><span style="font-size:10.0pt;">.</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"></span></span><br> <strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">یافته&shy; های پژوهش:</span></span></em></strong> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">نتایج</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">سمیت</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">سلولی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بیانگر</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">آن</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">است</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">که</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">غلظت&shy; های</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مختلف</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">اسید آسکوربیک، مرگ سلولی در گروه هدف</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مقایسه</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">با</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">گروه</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">کنترل</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">طور</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">معنی&shy; داری افزایش یافت(</span></span><span dir="LTR"><span style="font-size:10.0pt;">P<0.05</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">). نتایج</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">تست </span></span><span dir="LTR"><span style="font-size:10.0pt;">Real Time PCR</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> حاکی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">آن</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بود</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">که</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">میزان</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بیان</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ژن </span></span><span dir="LTR"><span style="font-size:10.0pt;">Bcl2</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">Bax</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مقایسه</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">با</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ژن </span></span><span dir="LTR"><span style="font-size:10.0pt;">GAPDH</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> در اثر تیمار با اسید آسکوربیک افزایش معنی داری داشته است(</span></span><span dir="LTR"><span style="font-size:10.0pt;">P<0.05</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">).</span></span><br> <strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">بحث و نتیجه گیری:</span></span></em></strong> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">نتایج مطالعات نشان داد که اسید آسکوربیک نقش مهمی را در مهار رشد سلول های سرطانی دارد.</span></span><br> &nbsp; <em>Introduction: </em>Antioxidants have a great influence on the control of oxidative stress and free radicals; moreover, they cause the expression of apoptosis genes. Accordingly, they have an important role in preventing cancer. This study aimed to evaluate the effect of ascorbic acid on signaling pathways of Bcl2 and Bax genes in AGS cell lines.<br> &nbsp; <pre> <em>Materials & Methods:</em> In this study, the AGS cell line was treated with different concentrations of ascorbic acid at different time intervals. The effect of ascorbic acid on cell death was measured using the MTT method. Furthermore, RNA extraction and cDNA synthesis were performed in this study. Finally, the expression of Bcl2 and Bax genes was evaluated using a real-time polymerase chain reaction (PCR) method. <em>Ethics code:0000000215021053</em>.</pre> <pre> </pre> <pre> <em>Findings:</em> Considering the different concentrations of ascorbic acid, the results obtained from the cell toxicity indicated a significant increase in cell death in the test group, compared to the control group (P<0.05). The results of the real-time PCR test showed that the expression of Bcl2 and Bax gene was significantly increased due to treatment with ascorbic acid, compared to GAPDH (P<0.05).</pre> <pre> </pre> <pre> <em>Discussion & Conclusions:</em> The results show that ascorbic acid plays an important role in inhibiting the growth of cancer cells.</pre> سرطان معده, اسید آسکوربیک, تست MTT, Bcl2 و Bax Ascorbic acid, Gastric cancer, MTT test, Bcl2, Bax 65 73 http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-5555-1&slc_lang=fa&sid=1 yaser Asefi یاسر آصفی yaser.asefi@yahoo.com 9600319475328460041028 9600319475328460041028 Yes Dept of Genetics, Faculty of Science, Islamic Azad University, Ahar Branch, Ahar, Iran گروه ژنتیک، دانشکده علوم، دانشگاه آزاد اسلامی، واحد اهر، اهر، ایران Saeid Ghorbian سعید قربیان gorbian@gmail.com 9600319475328460041029 9600319475328460041029 No Dept of Genetics, Faculty of Science, Islamic Azad University, Ahar Branch, Ahar, Iran گروه ژنتیک، دانشکده علوم، دانشگاه آزاد اسلامی، واحد اهر، اهر، ایران