fa ارتباط بین پلی مورفیسم Mir-499 rs3746444(A/G) و سرطان تخمدان در جمعیت زنان ایران ژنتیک مولکولی genetic mlvkly پژوهشي Research <strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">مقدمه:</span></span></em></strong> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">سرطان</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">تخمدان یکی از شایع ترین سرطان ها در بین بانوان می باشد. بر اساس گزارش های متعددی، </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">&nbsp;MicroRNA-499</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">از جمله </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">MicroRNA</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> هایی است که در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ایجاد کارسینوم</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">تخمدان نقش</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">دارند. وجود پلی مورفیسم های تک نوکلئوتیدی در توالی های مرتبط با بیان </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">miRNA</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> ها می</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">توانند</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بر</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">احتمال</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">خطر ابتلا</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">سرطان</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">نیز</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">موثر</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">باشند</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">.</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> هدف</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">انجام</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">این</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مطالعه، بررسی ارتباط</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">میان</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">پلی مورفیسم تک</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">نوکلئوتیدی</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"> mir-499 rs3746444 </span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">&nbsp;و</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">سرطان</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">تخمدان در یک جمعیت زنان ایران است.</span></span><br> <strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">مواد و روش ها:</span></span></em></strong> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">این</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مطالعه</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">که</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">صورت موردی-شاهدی صورت گرفته است، از 35 بیمار مبتلا به سرطان تخمدان بیمارستان امام خمینی و انستیتو سرطان و 35 فرد سالم نمونه خون محیطی جمع آوری شد. ابتدا استخراج </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">DNA</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> انجام شد و سپس پلی مورفیـــسم با</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">روش </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Arms-PCR</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> مورد بررسی قرار گرفت. اطلاعات توسط نرم فزار </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">SPSS</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> مورد تجزیه و تحلیل قرار گرفت.</span></span><br> <strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">یافته های پژوهش:</span></span></em></strong> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بر اساس بررسی پلی مورفیسم</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">تک</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">نوکلئوتیدی </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">rs3746444</span></span></span> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">mir-499</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> بین دو گروه بیمار و کنترل، فراوانی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ژنوتیپ های</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">AA </span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">،</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"> AG </span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">GG</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">گروه</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بیمار به</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ترتیب 9/2 درصد،4/51 درصد و 7/45 درصد و</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">گروه</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">کنترل 3/14 درصد، 7/45 درصد</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">و 40 درصد برآورد</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">شد</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">.</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> </span></span><br> <strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">بحث و نتیجه گیری:</span></span></em></strong> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">با</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">توجه</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">نتایج مطالعه</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">انجام</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">شده، تفاوت معناداری بین فراوانی ژنوتیپ ها در نمونه های سالم و بیمار وجود نداشت </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">([&chi;2 (1, 70)=0.23, P=0.63])</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> گر چه بر اساس </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Odds Ratio</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> محاسبه شده، احتمال خطر ابتلا به سرطان تخمدان در ژنوتیپ </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">GG</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> بیشتر می باشد(</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Odds Ratio=1.26, 95% CI:0.49-3.26</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">).</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span> <em>Introduction:</em> Ovarian cancer is one of the most prevalent cancers among women. According to several reports micro RNA-499 is involved in the development of ovarian carcinoma. The presence of Single Nucleotide Polymorphisms (SNPs) in miRNA sequences can also affect the risk of cancer. The aim of this study was to investigate the association between SNP mir-499 rs3746444 and the ovarian cancer in the Iranian female population.<br> &nbsp;<br> <em>Materials & Methods:</em> In this case-control study, peripheral blood samples were taken from 35 patients with ovarian cancer referred to Imam Khomeini Hospital and Cancer Institute and 35 healthy individuals. Subsequently, the DNA was extracted, and the polymorphism was investigated using Arms- polymerase chain reaction method. The data was analyzed in SPSS software. <em>Ethics code:</em> IR.PNU.REC.1398.118<br> <em>Findings:</em> The results of investigating SNP rs3746444 mir-499 between the two groups of patients and control indicated that the genotype frequencies of AA, AG, and GG were % 2.9, % 51.4, and % 45.7 in the patients and %14.3, % 45.7, and % 40 in the controls, respectively.<br> &nbsp;<br> <em>Discussion & Conclusions: </em>According to the results of the study, there was no significant difference between the healthy and patient samples regarding the frequencies of genotypes ([&chi;2 (1, 70) =0.23, P=0.63]); however, according to odds ratio, the risk of developing ovarian cancer is higher in the GG genotype (Odds Ratio=1.26, 95% CI: 0.49-3.26).<br> &nbsp; سرطان تخمدان,rs3746444 , Mir-499 Ovarian cancer, Mir-499, Rs3746444 47 55 http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-5516-1&slc_lang=fa&sid=1 Nahid Masoudi Ashtiani ناهید مسعودی آشتیانی nahidmasoudi87@yahoo.com 9600319475328460042694 0000000331991815 No Dept of Biology, Payame Noor University, Ray Branch, Tehran, Iran گروه زیست شناسی، دانشگاه پیام نور، مرکز ری، تهران، ایران Kasra Esfahani کسری اصفهانی kasra13@nigeb.ac.ir 9600319475328460042695 0000000330797009 Yes National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران، ایران Fatemeh Shahbazi فاطمه شهبازی fatemehs2011@gmail.com 9600319475328460042696 0000000325382220 No Dept of Biology, Payame Noor University, Ray Branch, Tehran, Iran گروه زیست شناسی، دانشگاه پیام نور، مرکز ری، تهران، ایران