Journal of Ilam University of Medical Sciences
مجله دانشگاه علوم پزشکی ایلام
J. Ilam Uni. Med. Sci.
Medical Sciences
http://sjimu.medilam.ac.ir
96
journal96
1563-4728
2588-3135
doi
fa
jalali
1398
3
1
gregorian
2019
6
1
27
2
online
1
fulltext
fa
اثرات حفاظتی عصاره زعفران«Crocus Sativus» بر کاهش استرس
اکسیداتیو طی بیماری پارکینسون در رت های نر
The Protective Effects of Saffron Extract "Crocus sativus'' on Oxidative Stress Reduction in Male Rats
with Parkinson’s Disease
پژوهشي
Research
<strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">مقدمه:</span></span></em></strong> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بیماری</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">پارکینسون</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">شایع</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ترین</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بیماری</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">های</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">نورودژنراتیو</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مغز</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">می</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">باشد.</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">با</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">توجه به</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">اثرات</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">آنتی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">اکسیدانی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">قوی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">عصاره</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">زعفران</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">در حذف</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">رادیکال</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">های</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">آزاد،</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">این</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مطالعه</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">منظور بررسی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">اثرات</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">محافظتی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">عصاره</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">زعفران</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بر</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">کاهش</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">استرس</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">اکسیداتیو</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">رت</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">های</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">پارکینسونی پیش</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">تیمار</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">با</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">عصاره</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">زعفران</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">انجام</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">شد</span></span><span dir="LTR"><span style="font-size:10.0pt;">.</span></span><br>
<strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">مواد و روش ها:</span></span></em></strong> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">این</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مطالعه</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">تجربی،</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">تعداد</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">35</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">سر</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">رت</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">نر</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">با</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">وزن</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">تقریبی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">50</span></span><span style="font-family:cambria,serif;"><span style="font-size:10.0pt;">±</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">250 گرم به صورت</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">تصادفی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">5</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">گروه</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">شامل: کنترل،</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">شاهد (دریافت کننده</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">سالین+اسیدآسکوربیک)،</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">پارکینسونی(تزریق6-هیدروکسی دوپامین) و 2</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">گروه</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">پارکینسونی+پیش</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">تیمار</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">عصاره</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">زعفران(5 میکروگرم/رت و 10 میکروگرم/رت، به مدت 5 روز) مورد مطالعه قرار گرفتند. ایجاد</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مدل</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">پارکینسونی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">با تزریق</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">داخل</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مغزی 6-هیدروکسی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">دوپامین 5/2</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">میکروگرم</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بر</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">میکرولیتر)</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">صورت</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">گرفت. میزان فعالیت آنتی اکسیدانی کل(</span></span><span dir="LTR"><span style="font-size:10.0pt;">FRAP</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">)، سطوح مالون دی آلدئید(</span></span><span dir="LTR"><span style="font-size:10.0pt;">MDA</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">)، میزان</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">فعالیت</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">آنزیم</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">سوپرکسید</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">دیسموتاز(</span></span><span dir="LTR"><span style="font-size:10.0pt;">SOD</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">)</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">گلوتا</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">تیون</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">پروکسیداز(</span></span><span dir="LTR"><span style="font-size:10.0pt;">GPX</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">) در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مغز</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">میانی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">با</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">استفاده</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">از کیت</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مخصوص</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">اندازه</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">گیری</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">شد</span></span><span dir="LTR"><span style="font-size:10.0pt;">.</span></span><br>
<strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">یافته</span></span></em></strong> <strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">های پژوهش:</span></span></em></strong> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">میزان </span></span><span dir="LTR"><span style="font-size:10.0pt;">FRAP</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> بین گروه کنترل، گروه های دریافت کننده زعفران و گروه پارکینسونی تفاوت معنی داری را نشان داد(</span></span><span dir="LTR"><span style="font-size:10.0pt;">P<0.01</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">). عصاره</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">زعفران</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">طور</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">معنی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">داری</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">تولید</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مالون</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">دی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">آلدئید</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">را</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">کاهش</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">داد(</span></span><span dir="LTR"><span style="font-size:10.0pt;">P<0.01</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">) و میزان</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">فعالیت</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">آنزیم</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">سوپرکسید</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">دیسموتاز و</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">گلوتاتیون پروکسیداز</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">را</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مغز</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">میانی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">طور</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">معنی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">داری</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">افزایش</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">داد(</span></span><span dir="LTR"><span style="font-size:10.0pt;">P<0.05</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">). </span></span><br>
<strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">بحث و نتیجه</span></span></em></strong> <strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">گیری:</span></span></em></strong> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">به نظر</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">می</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">رسد</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بخشی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">اثر</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">حفاظتی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">عصاره</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">زعفران</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">برابر</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">سم-6 هیدروکسی دوپامین</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">طریق</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">افزایش</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ظرفیت</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">آنزیم</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">های</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">آنتی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">اکسیدان</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">کاهش</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">تولید</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">رادیکال</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">های</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">آزاد انجام</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">می</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">گیرد</span></span><span dir="LTR"><span style="font-size:10.0pt;">.</span></span><br>
<br>
<em>Introduction:</em> Parkinson's disease (PD) is among the most common neurodegenerative disorders. Given the powerful antioxidant effects of the saffron extract on the removal of free radicals, this study aimed to investigate the protective effects of the saffron extract on reducing oxidative stress in a rat model of Parkinson's disease pretreated with saffron.<br>
<br>
<em>Materials & Methods: </em>In this experimental study, 35 male rats weighing approximately 250±50 g were assigned randomly into five groups, namely control, sham (ascorbate-saline), Parkinsonian rats induced by 6-hydroxydopamine, Parkinsonian rats pretreated with saffron extract (5 μg/rat for 5 days), and Parkinsonian rats pretreated with saffron extract (10 μg/rat for 5 days). The rat models were subjected to intracerebroventricular injection of 6-hydroxydopamine (2.5μg/μl). Moreover, Total Antioxidant Activity (TAC), Malondialdehyde (MDA) level, the enzyme activity of superoxide dismutase (SOD), and Glutathione Peroxidase (GPX) were measured in the midbrain using a special kit.<br>
<em>Ethics code:</em> 52.13267<br>
<br>
<em>Findings:</em> According to the results, there were differences between the control group, the groups pretreated with saffron and Parkinsonian rats regarding the level of TAC (P<0.01). In addition, saffron extract significantly reduced the level of MDA production (P<0.01). Moreover, there was an increase in the activity levels of SOD and GPX in the midbrain (P<0.05).<br>
<br>
<em>Discussion & Conclusions:</em> It seems that a part of the protective effect of the saffron extract against 6-hydroxydopamine can be attributed to the property that increases the capacity of antioxidant enzymes and decreases the production of free radicals.<br>
پارکینسون, عصاره زعفران, استرس اکسیداتیو
Oxidative stress, Parkinson's disease, Saffron extract
148
158
http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-4298-1&slc_lang=fa&sid=1
Homeira
Hatami
حمیرا
حاتمی
h.hatami@tabrizu.ac.ir
9600319475328460039626
9600319475328460039626
Yes
Dept of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
گروه زیست شناسی جانوری، دانشکده علوم طبیعی، دانشگاه تبریز، تبریز، ایران
Golamreza
Dehghan
غلامرضا
دهقان
dehghan2001d@yahoo.com
9600319475328460039627
9600319475328460039627
No
Dept of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
گروه زیست شناسی جانوری، دانشکده علوم طبیعی، دانشگاه تبریز، تبریز، ایران
Fereshteh
Alavi
فرشته
علوی
h.hatami@tabrizu.ac.i
9600319475328460039628
9600319475328460039628
No
Dept of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
گروه زیست شناسی جانوری، دانشکده علوم طبیعی، دانشگاه تبریز، تبریز، ایران