fa بررسی فراوانی شیوع ژن ‌های ویرولانس Fim H و PAI در باکتری ‌های اشریشیاکلی جدا شده از عفونت‌ های ادراری در شهر رشت پژوهشي Research <p dir="RTL" style="text-align: justify;"><strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">مقدمه:</span></span></em></strong> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">اشریشیاکلی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مهم ‌ترین</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">عوامل</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ایجاد کننده</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">عفونت</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مجاری</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ادراری</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">است.</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">سویه ‌های این باکتری</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">انواع</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مختلفی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">از فاکتورهای</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ویرولانس</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">جمله</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">چسبنده‌ ها،</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">توکسین‌ ها</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">سیستم ‌های</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">جذب</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">آهن</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">را</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">دارا</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">می ‌باشند.</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ژن ‌های</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ویرولانس</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بر روی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">عناصر</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ژنتیکی متحرک</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">یا</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">نواحی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">خاصی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">کروموزوم</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">که</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">جزایر</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بیماری زایی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">نامیده</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">می‌ شوند،</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">قرار</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">دارند. هدف</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">از این مطالعه بررسی فراوانی ژن‌ های ویرولانس </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Fim H</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">PAI</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> در باکتری‌ های اشریشیاکلی جدا شده از عفونت‌ های ادراری در شهر رشت بوده است.</span></span><br> <strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">مواد و روش ‌ها:</span></span></em></strong> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">این مطالعه بر روی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">50 سویه</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">اشریشیاکلی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">جدا شده</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بیماران</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مبتلا</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">عفونت</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ادراری مراجعه کننده به چند آزمایشگاه تشخیص طبی شهر رشت</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">انجام</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">شد. باکتری اشریشیاکلی با</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">استفاده</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">تکنیک ‌های</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">بیوشیمیایی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">میکروب ‌شناسی استاندارد شناسایی شد و فراوانی ژن‌ های</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ویرولانس </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Fim H</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">PAI</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> با</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">استفاده</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">آزمایش </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">PCR</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> بررسی گردید.</span></span><br> <strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">یافته های پژوهش:</span></span></em></strong> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">نتایج نشان داد که 76</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> درصد از زنان و 24</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">&shy;</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> درصد از مردان مبتلا به عفونت مـــجاری ادراری بودنـــد(</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P<0.01</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">) که نشان ‌دهنده شایع ‌تر بودن عفونت ادراری در زنان نسبت به مردان می ‌باشد</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">.</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> بر اساس نتایج آزمایش </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">PCR</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> میزان فراوانی ژن </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Fim H</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">، 42 مورد(84 درصد) و ژن </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">PAI</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">، 38 مورد(76 درصد) بود و در 33 مورد هر دو ژن </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Fim H</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">PAI</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> (66 درصد) شناسایی شدند.</span></span><br> <strong><em><span style="font-family:b mitra;"><span style="font-size:10.0pt;">بحث و نتیجه‌ گیری:</span></span></em></strong> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مطالعه</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">حاضر</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">نشان</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">داد</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">که</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">شیوع</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ژن‌ های</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ویرولانس </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Fim H</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">PAI</span></span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">میان</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">سویه‌ های اشریشیاکلی عامل عفونت ادراری بالا</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">می ‌باشد. بنا بر این،</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">ژن‌ های</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">فوق</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">می‌ توانند</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">به‌ عنوان</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">هدف</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مداخلات</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">درمانی</span></span> <span style="font-family:b mitra;"><span style="font-size:10.0pt;">مورد برررسی بیشتری قرار گیرند.</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;"></span></span></span></p> <p dir="RTL"></p> <div> <hr align="left" size="1" width="33%" > <div id="ftn1"><p></p></div> <div id="ftn2"></div></div> <p></p> <p style="text-align: justify;"><em>Introduction:</em> Uropathogenic Escherichia coli (UPEC) is one of the most important etiologic agents of urinary tract infection (UTI). The UPEC strains have various types of virulence factors, including adhesions, toxins, and iron uptake systems. Virulence genes are located on transmissible genetic elements and/or on particular locus on the chromosome called pathogenicity islands (PAI). The aim of the current research was to evaluate the frequency of PAI and Fim H virulence genes among E. coli strains isolated from UTIs in Rasht, Iran.<br> &nbsp;<br> <em>Materials and Methods:</em> This study was conducted on 50 E. coli strains, which were isolated from patients with UTIs referring to several medical laboratories of Rasht city. E. coli was identified using standard microbiological techniques and biochemical assays. Furthermore, the prevalence of PAI and Fim H virulence genes were evaluated using the polymerase chain reaction (PCR) technique.<br> &nbsp;<br> <em>Results:</em> The results showed that 76% of the females and 24% of the males were infected with UTI (P<0.01), which was indicative of the higher rate of UTIs in females than in males. Based on the results of PCR, 42 (84%) and 38 (76%) isolates were positive for Fim H and PAI genes, respectively. Additionally, 33 (66%) isolates carried both of these genes.<br> &nbsp;<br> <em>Conclusion:</em> As the findings indicated, the prevalence of PAI and Fim H virulence genes in E. coli strains accounted for a high rate of UTI. Therefore, these genes could be studied as targets for medical interventions and epidemiological studies.<br> &nbsp;<br> &nbsp;</p> اشریشیاکلی, PAI, Fim H, عفونت مجاری ادراری Escherichia coli, PAI, Fim H, Urinary tract infection 63 71 http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-3622-1&slc_lang=fa&sid=1 Zeinab Ghasemi pour زینب قاسمی پور zhila.ghs@gmail.com 9600319475328460039165 9600319475328460039165 No Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran گروه زیست شناسی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران Ali Salehzadeh علی صالح زاده salehzadehmb@yahoo.com 9600319475328460039166 9600319475328460039166 Yes Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran گروه زیست شناسی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران Hojatollah Zamani حجت اله زمانی h_zamani@guilan.ac.ir 9600319475328460039167 9600319475328460039167 No Department of Biology, Faculty of Sciences, Guilan University, Rasht, Iran گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران