fa تاثیر عصاره آبی چای سفید بر سطح آنزیم ‌های آنتی ‌اکسیدانی بافت کبددر موش‌های صحرایی‌ مواجهه شده با آرسنیک پژوهشي Research <p></p> <p>&nbsp;<strong><em><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">مقدمه :</span></span></span></em></strong><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;"> فلاوونوئید ها و ترکیبات فنلی ظرفیت بالای آنتی اکسیدانی و نقش مهم در سلامتی دارند و باعث افزایش سیستم دفاع آنتی ‌اکسیدانی در برابر استرس اکسیداتیو می گردند. چای سفید نیز از جمله گیاهانی هست که به دلیل ظرفیت آنتی ‌اکسیدانی بالا اخیراً مورد توجه قرار گرفته است. هدف از مطالعه حاضر بررسی تاثیر چای سفید بر استرس اکسیداتیو ناشی از آرسنیک می‌باشد</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;">.</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"></span></span><br> <strong><em><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">مواد و روش ها :</span></span></span></em></strong> <span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">در این مطالعه از 32 موش صحرایی نر بالغ در چهار گروه هشت ‌تایی استفاده شد. گروه اول موش ‌های صحرایی سالم (گروه شاهد) که آب مقطر را روزانه به همراه رژیم غذایی استاندارد به صورت گاواژ دریافت کردند. گروه دوم موش‌ های صحرایی تیمار‌شده با آرسنیک با دوز</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;"> (mg/L) 50 </span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">در آب آشامیدنی، گروه سوم موش ‌های صحرایی تیمار‌شده با عصاره چای سفید با غلظت 5/1 درصد به صورت گاواژ و گروه چهارم موش ‌های صحرایی که عصاره آبی چای سفید (5/1 درصد) را از طریق گاواژ همراه با آرسنیک</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;"> (mg/L) 50 </span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">&nbsp;در آب آشامیدنی دریافت کردند. بعد از پایان دوره تیمار &nbsp;(28 روز) رت ها با استفاده از اتر بی هوش شده و سپس کالبدگشائی انجام شد و سطح آنزیم ‌های آنتی ‌اکسیدانی کاتالاز</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;"> (CAT) </span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">گلوتاتیون پر اکسیداز</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;"> (GPx) </span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">و سوپر اکسید دیسموتاز</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;"> (SOD) </span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">، ظرفیت تام آنتی ‌اکسیدانی</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;"> (TAC) </span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">و شاخص پر اکسیداسیون لیپیدی</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;"> (MDA) </span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">کبدی مورد بررسی قرار گرفت.</span></span></span><br> <strong><em><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">یافته های پژوهش:</span></span></span></em></strong><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;"> عصاره آبی چای سفید باعث افرایش معنی ‌دار فعالیت آنزیم‌ های</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;"> CAT</span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">، </span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;">&nbsp;GPx </span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">و</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;"> SOD </span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">و کاهش معنی ‌دار</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;"> MDA </span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">&nbsp;و افزایش</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;"> TAC </span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">گردید (</span></span></span><span style="color:black;"><span style="font-family:b mitra;">5 0/0</span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P<</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"> ) و مصرف آرسنیک سدیم نیز باعث کاهش معنی دار<span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"> فعالیت آنزیم‌ های</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;"> CAT</span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">، </span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;">&nbsp;GPx </span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">و</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;"> SOD</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"> <span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;">و افزایش معنی ‌دار</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;"> MDA </span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">&nbsp;و افزایش</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;"> TAC</span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;"> گردید (</span></span></span><span style="color:black;"><span style="font-family:b mitra;">5 0/0</span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P<</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;">) و مصرف <span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;">عصاره آبی چای سفید </span>همراه آرسنیک سدیم باعث افزایش فعالیت معنی دار</span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;"> GPx </span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">و </span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;">&nbsp;SOD</span></span></span></span><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;"> گردید (</span></span></span><span style="color:black;"><span style="font-family:b mitra;">5 0/0</span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P<</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;">).</span></span><br> <strong><em><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;">&nbsp;بحث و نتیجه گیری :</span></span></span></em></strong><span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:b mitra;"> نتایج نشان داد که مصرف چای سفید با افزایش فعالیت آنزیم‌ های آنتی ‌اکسیدانی و تقویت سیستم دفاع آنتی ‌اکسیدانی باعث کاهش استرس اکسیداتیو ناشی از آرسنیک می ‌گردد</span></span></span><span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;">.</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"> در بررسی حاضر نیزآرسنیت سدیم باعث کاهش فعالیت&shy;</span></span> <span style="color:black;"><span style="font-family:b mitra;">های آنزیم&shy;</span></span> <span style="color:black;"><span style="font-family:b mitra;">های آنتی &shy;اکسیدان و افزایش پر اکسیداسیون لیپیدی شد</span></span> <span style="color:black;"><span style="font-family:b mitra;">.</span></span></p> <p></p> <p><strong><em>Introduction</em></strong>: Having high antioxidant capacity and an important role in the health, the flavonoids and phenolic compounds increase antioxidant defense against antioxidative stress. White tea is among herbs attracting attention recently due to high antioxidant capacity. The present study aimed at surveying the effect of white tea on oxidative stress resulted of Arsenic.<br> &nbsp;<br> <strong><em>Materials & methods</em></strong>: In the present study 32 male rats were used in four groups of eight animals. The first group composed of healthy animals (control group) which received distilled water along with standard dietary via gavage, the second one was treated with Arsenic (<span dir="RTL">50</span> ppm in drinking water), the third group was treated with white tea extract (1.5%) via gavage and the fourth one received aqueous white tea extract (1.5%) plus Arsenic (<span dir="RTL">5</span>0 ppm in drinking water) via gavage. Upon the end of the treatment period (28 days) the rats were anesthetized using ether and then autopsy was conducted to measure the levels of Catalase Antioxidant Enzymes (CAT), Glutathione Peroxidase (GPx), Super Oxide Dismutase (SOD), Total Antioxidant Capacity (TAC), and Malondialdehyde (MDA) of liver.<br> &nbsp;<br> <strong><em>Findings</em></strong>: The aqueous extract of white tea caused a meaningful increase of CAT , GPx, and SOD activities as well as a meaningful decrease of MDA . It also increased the TAC (P<0.05). On the other hand, administrating the Sodium Arsenic caused a meaningful decrease of&nbsp;&nbsp;&nbsp; CAT , GPx, and SOD as well as a meaningful increase of MDA . It also increased the TAC (P<0.05). Administrating the aqueous extract of white tea plus Sodium Arsenic caused a meaningful increase of SOD AND GPx activities (P<0.05).<br> &nbsp;<br> <strong><em>Discussion & conclusion</em></strong><strong><em>s</em></strong>: The results demonstrated that administration of white tea causes to increase the antioxidant enzymes activity as well as to strengthen the antioxidant defense and to decrease the stress oxidative resulted of Arsenic, besides. In the present the administration of Sodium Arsenite caused decreased antioxidant enzymes activity and increased MDA.<br> &nbsp;</p> آرسنیک, آنزیم ‌های آنتی ‌اکسیدان, استرس اکسیداتیو, چای سفید Arsenic, Antioxidant enzymes, Oxidative stress, White tea 146 153 http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-3276-2&slc_lang=fa&sid=1 Mohammad Hasan Rasolifard محمد حسن رسو لی فرد rasoulifardmohamadhassan@yahoo.com 9600319475328460038443 9600319475328460038443 Yes Dept of Food and Aquaculture Hygiene, Tabriz Branch, Islamic Azad University, Tabriz, Iran گروه آموزشی بهداشت مواد غذایی و آبزیان، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران Yaser Nozohor یاسر نوظهور yasar_nozohour@yahoo.com 9600319475328460038444 9600319475328460038444 No Young Researchers and Elites Club, Tabriz Branch, Islamic Azad University, Tabriz, Iran باشگاه پژوهشگران جوان و نخبگان، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران