fa ارزیابی خواص ضد باکتریایی اسانس پونه کوهی برروی باکتری های پاتوژن جدا شده ازعفونت های بیمارستانی پژوهشي Research <strong><em><span style="font-family:b mitra;">مقدمه</span></em></strong><strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;"> :</span></span></span></em></strong> <span style="font-family:b mitra;">استفاده</span> <span style="font-family:b mitra;">از</span> <span style="font-family:b mitra;">خواص</span> <span style="font-family:b mitra;">ضد</span> <span style="font-family:b mitra;">میکروبی</span> <span style="font-family:b mitra;">گیاهان</span> <span style="font-family:b mitra;">دارویی</span> <span style="font-family:b mitra;">می</span> <span style="font-family:b mitra;">تواند</span> <span style="font-family:b mitra;">مشکلات</span> <span style="font-family:b mitra;">رایج</span> <span style="font-family:b mitra;">در</span> <span style="font-family:b mitra;">استفاده</span> <span style="font-family:b mitra;">از</span> <span style="font-family:b mitra;">آنتی</span> <span style="font-family:b mitra;">بیوتیک</span> <span style="font-family:b mitra;">ها</span> <span style="font-family:b mitra;">را</span> <span style="font-family:b mitra;">بر طرف</span> <span style="font-family:b mitra;">کند</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">. </span></span></span><span style="font-family:b mitra;">&nbsp;هدف</span> <span style="font-family:b mitra;">از انجام </span>&nbsp;<span style="font-family:b mitra;">این</span> <span style="font-family:b mitra;">مطالعه،</span> <span style="font-family:b mitra;">ارزیابی</span> <span style="font-family:b mitra;">خواص</span> <span style="font-family:b mitra;">ضد</span> <span style="font-family:b mitra;">باکتریایی</span> <span style="font-family:b mitra;">اسانس</span> <span style="font-family:b mitra;">پونه</span> <span style="font-family:b mitra;">کوهی</span> <span style="font-family:b mitra;">بر</span> <span style="font-family:b mitra;">روی</span> <span style="font-family:b mitra;">باکتری</span> <span style="font-family:b mitra;">های</span> <span style="font-family:b mitra;">جداشده</span> <span style="font-family:b mitra;">از</span> <span style="font-family:b mitra;">عفونت</span> <span style="font-family:b mitra;">بیمارستانی</span> <span style="font-family:b mitra;">می</span> <span style="font-family:b mitra;">باشد</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">.</span></span></span><br> <strong><span style="font-family:b mitra;">مواد و روش</span></strong> <strong><span style="font-family:b mitra;">ها</span></strong> : <span style="font-family:b mitra;">در</span> <span style="font-family:b mitra;">این</span> <span style="font-family:b mitra;">مطاله</span> <span style="font-family:b mitra;">تجربی</span> <span style="font-family:b mitra;">اسانس</span> <span style="font-family:b mitra;">پونه</span> <span style="font-family:b mitra;">کوهی</span> <span style="font-family:b mitra;">با</span> <span style="font-family:b mitra;">استفاده</span> <span style="font-family:b mitra;">از</span> <span style="font-family:b mitra;">دستگاه</span> <span style="font-family:b mitra;">کلونجر</span> <span style="font-family:b mitra;">تهیه</span> <span style="font-family:b mitra;">گردید و</span>&nbsp; <span style="font-family:b mitra;">سپس</span> <span style="font-family:b mitra;">فعالیت</span> <span style="font-family:b mitra;">ضد</span> <span style="font-family:b mitra;">باکتریایی</span> <span style="font-family:b mitra;">به</span> <span style="font-family:b mitra;">روش</span> <span style="font-family:b mitra;">انتشار</span> <span style="font-family:b mitra;">در</span> <span style="font-family:b mitra;">آگار</span> <span style="font-family:b mitra;">جهت</span> <span style="font-family:b mitra;">انجام</span> <span style="font-family:b mitra;">آزمایش</span> <span style="font-family:b mitra;">حساسیت</span> <span style="font-family:b mitra;">میکروبی</span>&nbsp; <span style="font-family:b mitra;">و از</span> <span style="font-family:b mitra;">روش</span> <span style="font-family:b mitra;">رقت</span>&nbsp; <span style="font-family:b mitra;">لوله</span> <span style="font-family:b mitra;">ای</span> <span style="font-family:b mitra;">جهت&nbsp; تعیین</span>&nbsp; <span style="font-family:b mitra;">حداقل</span> <span style="font-family:b mitra;">غلظت</span> <span style="font-family:b mitra;">ممانعت</span> <span style="font-family:b mitra;">از رشد </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">&nbsp;( </span></span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">MIC )</span></span></span> &nbsp;<span dir="LTR" style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:#222222;"><span style="font-family:times new roman,serif;"><span style="font-size:9pt;">Minimum Inhibitory Concentration</span></span></span></span><span style="font-family:b mitra;"> &nbsp;و حداقل غلظت باکتری کشی</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;"> ( MBC)</span></span></span> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">&nbsp;<span style="background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"><span style="color:#222222;">Minimum Bactericidal Concentration</span></span></span></span></span><span style="font-family:b mitra;"> به طور جدا گانه استفاده گردید</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">.</span></span></span><br> <strong><em><span style="font-family:b mitra;">یافته های پژوهش</span></em></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;"> :&nbsp; </span></span></span></strong><span style="font-family:b mitra;">آزمایش</span> <span style="font-family:b mitra;">حساسیت</span> <span style="font-family:b mitra;">میکروبی</span> <span style="font-family:b mitra;">در</span> <span style="font-family:b mitra;">موردآنتی</span> <span style="font-family:b mitra;">بیوتیک های</span> <span style="font-family:b mitra;">استاندارد</span> <span style="font-family:b mitra;">تترا سایکلین</span> <span style="font-family:b mitra;">و</span> <span style="font-family:b mitra;">آمیکاسین</span> <span style="font-family:b mitra;">و</span> <span style="font-family:b mitra;">نیز</span> <span style="font-family:b mitra;">دیسک</span> <span style="font-family:b mitra;">آغشته</span> <span style="font-family:b mitra;">به</span> <span style="font-family:b mitra;">اسانس</span> <span style="font-family:b mitra;">پونه</span> <span style="font-family:b mitra;">کوهی</span> <span style="font-family:b mitra;">نشان</span> <span style="font-family:b mitra;">داد</span> <span style="font-family:b mitra;">که</span> <span style="font-family:b mitra;">قطر</span> <span style="font-family:b mitra;">منطقه</span> <span style="font-family:b mitra;">عدم</span> <span style="font-family:b mitra;">رشد</span> <span style="font-family:b mitra;">ایجادشده</span> <span style="font-family:b mitra;">توسط</span> <span style="font-family:b mitra;">این</span> <span style="font-family:b mitra;">ترکیب</span> <span style="font-family:b mitra;">در</span> <span style="font-family:b mitra;">مورد</span> <span style="font-family:b mitra;">4</span> <span style="font-family:b mitra;">جدایه</span> <span style="font-family:b mitra;">ای</span> <span style="font-family:b mitra;">مورد</span> <span style="font-family:b mitra;">آزمایش</span> <span style="font-family:b mitra;">بزرگ تر</span> <span style="font-family:b mitra;">و</span> <span style="font-family:b mitra;">در</span> <span style="font-family:b mitra;">مورد</span> <span style="font-family:b mitra;">اشریشیا</span> <span style="font-family:b mitra;">کلی</span> <span style="font-family:b mitra;">وکلبسیلا</span> <span style="font-family:b mitra;">نومونیا</span> <span style="font-family:b mitra;">این</span> <span style="font-family:b mitra;">اختلاف</span> <span style="font-family:b mitra;">اندازه</span> <span style="font-family:b mitra;">معنی</span> <span style="font-family:b mitra;">داری</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">) </span></span></span><span style="font-family:b mitra;">5 0/0</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P<</span></span></span><span style="font-family:b mitra;">)</span><span style="font-family:b mitra;"> بود.</span> <span style="font-family:b mitra;">هم چنین</span> <span style="font-family:b mitra;">نتایج</span> <span style="font-family:b mitra;">حاصل</span> <span style="font-family:b mitra;">از</span> <span style="font-family:b mitra;">آزمایشات</span> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">MIC </span></span></span><span style="font-family:b mitra;">و </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">MBC </span></span></span><span style="font-family:b mitra;">&nbsp;هم مشخص کرد که میزان مهار رشد باکتری توسط اسانس با میزان اسانس موجود در رقت ها رابطه مستقیم دارد و با افزایش میزان اسانس در هر رقت از تعداد کلنی های باکتری بعد از کشت کاسته شده و در رقتی که نشان دهنده </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">&nbsp;MBC </span></span></span><span style="font-family:b mitra;">اسانس پونه کوهی&nbsp; در مورد هر یک از جدایه ها بود، هیچ یک از آن ها اصلاً رشد نکرد</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">.</span></span></span><br> <strong><span style="font-family:b mitra;">بحث</span></strong> <strong><span style="font-family:b mitra;">و</span></strong> <strong><span style="font-family:b mitra;">نتیجه</span></strong> <strong><span style="font-family:b mitra;">گیری</span></strong> : <span style="font-family:b mitra;">نتایج</span> <span style="font-family:b mitra;">به</span> <span style="font-family:b mitra;">دست</span> <span style="font-family:b mitra;">آمده</span> <span style="font-family:b mitra;">نشان</span> <span style="font-family:b mitra;">داد</span>&nbsp; <span style="font-family:b mitra;">که</span> <span style="font-family:b mitra;">اسانس</span> <span style="font-family:b mitra;">پونه</span> <span style="font-family:b mitra;">کوهی</span> <span style="font-family:b mitra;">دارای</span> <span style="font-family:b mitra;">خاصیت</span> <span style="font-family:b mitra;">ضد</span> <span style="font-family:b mitra;">باکتریایی</span> <span style="font-family:b mitra;">می</span> <span style="font-family:b mitra;">باشد</span> <span style="font-family:b mitra;">و</span> <span style="font-family:b mitra;">می</span> <span style="font-family:b mitra;">تواند</span> <span style="font-family:b mitra;">به عنوان</span> <span style="font-family:b mitra;">یک</span> <span style="font-family:b mitra;">منبع</span> <span style="font-family:b mitra;">ارزان</span> <span style="font-family:b mitra;">و</span> <span style="font-family:b mitra;">در</span> <span style="font-family:b mitra;">دسترس</span> <span style="font-family:b mitra;">برای</span> <span style="font-family:b mitra;">استفاده</span> <span style="font-family:b mitra;">درمانی</span> <span style="font-family:b mitra;">در</span> <span style="font-family:b mitra;">برخی</span> <span style="font-family:b mitra;">از</span> <span style="font-family:b mitra;">عفونت های</span> <span style="font-family:b mitra;">باکتریایی</span> <span style="font-family:b mitra;">مورد</span> <span style="font-family:b mitra;">استفاده</span> <span style="font-family:b mitra;">قرار</span> <span style="font-family:b mitra;">گیرد</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;"> .</span></span></span><span style="font-family:b mitra;">به عبارتی</span> <span style="font-family:b mitra;">می</span> <span style="font-family:b mitra;">توان</span> <span style="font-family:b mitra;">از</span> <span style="font-family:b mitra;">پونه</span> <span style="font-family:b mitra;">کوهی</span> <span style="font-family:b mitra;">به</span> <span style="font-family:b mitra;">عنوان</span> <span style="font-family:b mitra;">جایگزین</span> <span style="font-family:b mitra;">مناسب</span> <span style="font-family:b mitra;">برای</span> <span style="font-family:b mitra;">انتی</span> <span style="font-family:b mitra;">بیوتیک</span> <span style="font-family:b mitra;">های</span> <span style="font-family:b mitra;">صناعی</span> <span style="font-family:b mitra;">جهت</span> <span style="font-family:b mitra;">مقابله</span> <span style="font-family:b mitra;">با</span> <span style="font-family:b mitra;">جدایه</span> <span style="font-family:b mitra;">های</span> <span style="font-family:b mitra;">مورد</span> <span style="font-family:b mitra;">آزمایش</span> <span style="font-family:b mitra;">در</span> <span style="font-family:b mitra;">نظر</span> <span style="font-family:b mitra;">گرفته</span> <span style="font-family:b mitra;">شود</span><span style="font-family:b mitra;"></span><br> &nbsp; <p dir="RTL"></p><p dir="RTL"></p><p dir="RTL"></p> <p dir="RTL" style="text-align: left;"><strong><em>Introduction</em></strong>: The use of antimicrobial properties of medicinal plants can solve common problems in the use of antibiotics. The aim of this study was to evaluate the antibacterial properties of the Mentha longifolia essence on bacteria isolated from hospital infections.<br> &nbsp;<br> <strong><em>Materials & methods</em></strong><strong>:</strong> In this experimental study the Mentha longifolia essence was extracted using Clevenger device and its antimicrobial activity agar disk diffusion method for determination of bacterial sensitivity and dilution method for determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was used separately.<br> &nbsp;<br> <strong><em>Findings</em></strong>: The results of bacterial sensitivity test in the case of the standard antibiotics of tetracycline, amikacine, and also disks impregnated with Mentha longifolia essence showed that the diameter of the zone of inhibition caused by the compound being tested on four strains of isolates was far larger and this difference about Escherichia coli and Klebsiella isolates was significant (p<0.05). Also the results of MIC and MBC tests indicated that the inhibition of bacterial growth by Mentha longifolia essence had a direct relationship with the amount of available Mentha longifolia essence in the dilution and increasing of Mentha longifolia essence in each dilution reduced the number of cultured bacterial colonies and no bacterial growth was observed in the dilution equivalent to MBC of Mentha longifolia essence.<br> &nbsp;<br> <strong><em>Discussion & conclusion</em></strong><strong><em>s</em></strong><strong>:</strong> The results suggest that Mentha longifolia essence can be used as a source of cheap and accessible replacing chemical drugs to treat some bacterial infections.<br> &nbsp;</p> ضد باکتریایی , اسانس , پونه کوهی , عفونت های بیمارستانی Antibacterial, Mentha longifolia, Hospital infections 154 160 http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-3276-1&slc_lang=fa&sid=1 Yaser Nozohor یاسر نوظهور yasar_nozohour@yahoo.com 9600319475328460038445 9600319475328460038445 Yes Dept of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran گروه دام پزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران Mohammad Hasan Rasolifard محمد حسن رسولی فرد rasoulifardmohamadhassan@yahoo.com 9600319475328460038446 9600319475328460038446 No Dept of Food and Aquaculture Hygiene, Tabriz Branch, Islamic Azad University, Tabriz, Iran گروه آموزشی بهداشت مواد غذایی و آبزیان، واحد تبریز،دانشگاه آزاد اسلامی، تبریز، ایران Nahideh Ghahremanigermi ناهیده قهرمانی گرمی ghahramani_n49@yahoo.com 9600319475328460038447 9600319475328460038447 No Dept of Nursing, Ardabil Branch, Islamic Azad University, Ardebil, Iran گروه پرستاری ، واحد اربیل ، دانشگاه آزاد اسلامی، اردبیل، ایران