fa بررسی ارتباط بین پلی‌ مورفیسم rs4938723 در ژن mir-34 b/c و خطر ابتلا به نا باروری آیدیو پاتیک در مردان پژوهشي Research <pre dir="RTL" style="text-align:justify;direction:rtl;unicode-bidi:embed;background-image:initial;background-attachment:initial;background-size:initial;background-origin:initial;background-clip:initial;background-position:initial;background-repeat:initial;"> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نا</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">باروری، اختلال پیچیده‌ای است که با علل متعدد ژنتیکی و محیطی همراه می&shy;باشد.</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">نا باروری</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">مردا</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ن، ت</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">قریبا</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">15</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">درصد </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">از</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">زوجین</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">را درگیر می&shy;کند.</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">میکرو &shy;</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">RNA</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">&shy;‌ها نقش مهمی در فرایند اسپرماتوژنز دارند؛ اما این که آیا پلی</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">&shy;مورفیسم&shy;‌های </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">pre-miRNA</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> با نا باروری آیدیو پاتیک مردان همراه هستند، هنوز نا مشخص باقی مانده است. در این مطالعه، </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ارتباط بین پلی</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">&shy;مورفیسم </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">rs4938723</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در ژن </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">mir-34 b/c</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و خطر ابتلا به نا</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">باروری آیدیو پاتیک در مردان، مورد بررسی قرار گرفت.</span></span></pre> <p dir="RTL"><span dir="LTR"><strong><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مواد</span></span></span></strong> <strong><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">و</span></span></span></strong> <strong><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">روش&shy;‌ ها: </span></span></span></strong><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نمونه&shy;‌ های</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">خون</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">از</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">244</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مرد</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مبتلا</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">به</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نا باروری</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">آیدیو پاتیک</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">و</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">105</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مرد</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">سالم</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">جمع</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">آوری</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">شد. استخراج </span></span></span><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">DNA</span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> با روش </span></span></span><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">Salting out</span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> انجام شد و تعیین ژنوتیپ به&shy; وسیله تکنیک</span></span></span> <span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">Tetra ARMS PCR</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">صورت گرفت. هم&shy; چنین</span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، </span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مقایسه فراوانی</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ژنوتیپی</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">و</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">آللی</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">در</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">دو</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">گروه بیمار</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">و</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">کنترل</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">به &shy;وسیله</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">آزمون</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">رگرسیون لوجس</span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">تیک انجام پذیرفت. </span></span></span><br> <strong><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">یافته &shy;‌های پژوهش: </span></span></span></strong><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هر دو گروه بیمار (17/0</span></span></span><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">P=</span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، 2</span></span></span><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">df=</span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، 8/1</span></span></span><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">&chi;2=</span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) و کنترل (2/0</span></span></span><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">P=</span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، 2</span></span></span><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">df=</span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، 6/1</span></span></span><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">&chi;2=</span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) برای توزیع ژنوتیپ ‌ها در تعادل‌ هاردی- واینبرگ بودند. در مدل ژنتیک مغلوب برای ژنوتیپ </span></span></span><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">CC</span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، پلی&shy; مورفیسم </span></span></span><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">rs4938723</span></span></span> <span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">mir-34 b/c</span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> ارتباط معنی&shy; داری را با نا باروری آیدیو پاتیک در مردان نشان داد (03/0</span></span></span><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">P=</span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، 9/0-2/0</span></span></span><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">95%CI=</span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، 44/0</span></span></span><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">OR=</span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)</span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">.</span></span></span><br> <strong><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">بحث و نتیجه &shy;گیری</span></span></span></strong><strong><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">:</span></span></span></strong> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">در این مطالعه برای اولین بار، ارتباط بین پلی&shy; مورفیسم </span></span></span><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">mir-34 b/c rs4938723</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">با </span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نا باروری آیدیو پاتیک مردان</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">بررسی شد. نتایج</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نشان</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">داد</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">که</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">پلی</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مورفیسم </span></span></span><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">mir-34 b/c rs4938723</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">با </span></span></span><span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نا</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">باروری آیدیو پاتیک مردان</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">در</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ارتباط</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">است؛</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">اگر چه</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">به</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">منظور</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">تایید</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">این</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج،</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">باید</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مطالعات</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">گسترده &shy;تری</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">با</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">افراد</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">کنترل</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">و</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">بیمار</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">بیش&shy;تر صورت</span></span></span> <span style="color:windowtext;"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">گیرد</span></span></span><span dir="LTR"><span style="color:windowtext;"><span style="font-size:9.0pt;">.</span></span></span></span></p> <p><em>Introduction</em>: Infertility is a complex disorder with multiple genetic and environmental causes. Male factor infertility affects 15% of all couples. MicroRNA plays an important role in spermatogenesis. Whether pre-miRNAs polymorphisms are associated with idiopathic male infertility is unknown. In this study, the association between mir-34 b/c rs4938723 gene polymorphism and the risk of idiopathic male infertility was investigated.<br> &nbsp;<br> <em>Materials & methods</em>: Blood Samples were collected from 244 patients diagnosed with idiopathic male infertility and 105 control subjects. DNA extraction was performed by salting out method and genotype determination was done by Tetra-ARMS PCR technique. However, the comparison between genotype and allele frequencies in cases and controls, were assessed by logistic regression analysis.<br> &nbsp;<br> <em>Findings</em>: Both of the cases <span dir="RTL">)</span>&chi;²= 1.8, df=2, P=0.17<span dir="RTL">(</span> and control <span dir="RTL">)</span>&chi;²= 1.6, df=2, P=0.2<span dir="RTL">(</span> groups were in Hardy-Weinberg equilibrium for genotype distribution. In a recessive genetic model for CC genotype, mir-34 b/c rs4938723 showed significant association with idiopathic male infertility (OR= 0.44, 95% CI= 0.2-0.9, P= 0.03).<br> &nbsp;<br> <em>Discussion & conclusions</em>: In this study, for the first time, the association between mir-34 b/c rs4938723 and idiopathic male infertility was investigated. The results showed that mir-34 b/c rs4938723 polymorphism is associated with idiopathic male infertility; more studies should be considered with larger number of patient and control subjects to confirm these results.</p> نا باروری مردان, mir-34 b/c, پلی‌مورفیسم, آیدیو پاتیک Male infertility, Mir-34 b/c, Polymorphism, Idiopathic 83 89 http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-2299-2&slc_lang=fa&sid=1 Hajar Majidi هاجر مجیدی fatimamajidi68@gmail.com 9600319475328460038693 9600319475328460038693 No Dept of Biology, Faculty of Science, Arsanjan branch, Islamic Azad University, Arsanjan, Iran گروه زیست شناسی، دانشکده علوم، واحد ارسنجان، دانشگاه آزاد اسلامی، ارسنجان، ایران Leila Kohan لیلا کهن leila_kohan@yahoo.com 9600319475328460038694 9600319475328460038694 Yes Dept of Biology, Faculty of Science, Arsanjan branch, Islamic Azad University, Arsanjan, Iran گروه زیست شناسی، دانشکده علوم، واحد ارسنجان، دانشگاه آزاد اسلامی، ارسنجان، ایران Athar Rasekh اطهر راسخ جهرمی Drrasekh@yahoo.com 9600319475328460038695 9600319475328460038695 No Dept of Obstetrics and Gynecology, Faculty of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran گروه زنان و زایمان، دانشکده پزشکی، دانشگاه علوم پزشکی جهرم، جهرم، ایران